LOCUS       V014568                10293 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014568
VERSION     V014568
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2050..2167
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2227..2726
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             2845..2874
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             2875..3315
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             3352..3501
                     /label="VP64"
                     /note="tetrameric repeat of the minimal activation domain
                     of herpes simplex virus VP16 (Beerli et al., 1998)"
     misc_feature    4157..4745
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             4855..5088
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5113..5131)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        5919..6023
                     /label="AmpR promoter"
     CDS             6024..6881
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7055..7643
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        7889..8218
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     intron          9352..9417
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9547..9567
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
     2041 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2101 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2161 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
     2221 cgaattgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     2281 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     2341 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     2401 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     2461 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     2521 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     2581 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     2641 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     2701 cctcttcctc atctccgggc ctttcgaatt ctcacgcgtc aagtggagca aggcaggtgg
     2761 acagtggatc gccacgccac catggcattg cccgtgaccg ccctgctgct gccactggcc
     2821 ttgttgctcc acgccgcgcg gccagaacag aagctgatca gcgaggagga tctgatgaag
     2881 ctgctgagca gcatcgagca ggcctgcgac atctgccgcc tgaagaagct gaagtgcagc
     2941 aaggagaagc ccaagtgcgc caagtgcctg aagaacaact gggagtgccg ctacagcccc
     3001 aagaccaagc gcagccccct gacccgcgcc cacctgaccg aggtggagag ccgcctggag
     3061 cgcctggagc agctgttcct gctgatcttc ccccgcgagg acctggacat gatcctgaag
     3121 atggacagcc tgcaggacat caaggccctg ctgaccggcc tgttcgtgca ggacaacgtg
     3181 aacaaggacg ccgtgaccga ccgcctggcc agcgtggaga ccgacatgcc cctgaccctg
     3241 cgccagcacc gcatcagcgc caccagcagc agcgaggaga gcagcaacaa gggccagcgc
     3301 cagctgaccg tgagcgccgc cgccggcggc agcggcggca gcggcggcag cgacgccctg
     3361 gacgacttcg acctggacat gctgggcagc gacgccctgg acgacttcga cctggacatg
     3421 ctgggcagcg acgccctgga cgacttcgac ctggacatgc tgggcagcga cgccctggac
     3481 gacttcgacc tggacatgct gggcagcaag cgcaagcgca agcacatcgc caccgtggtg
     3541 atcatcatca gcgtgtgcat gctggtgttc gtggtggcca tgggcgtgta caccaccacc
     3601 cccgcccccc gcccccccac ccccgccccc accatcgcca gccagcccct gagcctgcgc
     3661 cccgaggcct gcatggccga cgtgcagctg gtggagagcg gcggcggcct ggtgcaggcc
     3721 ggcggcagcc tgcgcctgag ctgcgccgcc agcggccgca ccatcagcat ggccgccatg
     3781 agctggttcc gccaggcccc cggcaaggag cgcgagttcg tggccggcat cagccgcagc
     3841 gccggcagcg ccgtgcacgc cgacagcgtg aagggccgct tcaccatcag ccgcgacaac
     3901 accaagaaca ccctgtacct gcagatgaac agcctgaagg ccgaggacac cgccgtgtac
     3961 tactgcgccg tgcgcaccag cggcttcttc ggcagcatcc cccgcaccgg caccgccttc
     4021 gactactggg gccagggcac ccaggtgacc gtgagctgag cggccgcaac tcccacctgc
     4081 aacatgcgtg actgactgag gccgcgactc tagagtcgac ctgcaggcat gcaagcttga
     4141 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4201 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4261 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4321 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4381 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4441 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4501 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4561 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4621 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4681 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4741 cccgcatcga taccgtcgac ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa
     4801 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     4861 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     4921 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4981 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5041 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt
     5101 aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta
     5161 ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt
     5221 actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg
     5281 aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag
     5341 cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     5401 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     5461 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     5521 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     5581 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat
     5641 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     5701 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     5761 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     5821 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5881 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5941 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6001 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6061 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     6121 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     6181 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     6241 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     6301 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     6361 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6421 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6481 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6541 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6601 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6661 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6721 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6781 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6841 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6901 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6961 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7021 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7081 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7141 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7201 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7261 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7321 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7381 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7441 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7501 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7561 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7621 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7681 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7741 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7801 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7861 ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7921 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7981 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8041 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8101 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     8161 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     8221 gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag
     8281 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
     8341 atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
     8401 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
     8461 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
     8521 taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     8581 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     8641 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     8701 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
     8761 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
     8821 tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     8881 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
     8941 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     9001 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     9061 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     9121 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     9181 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     9241 accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc
     9301 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     9361 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     9421 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     9481 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     9541 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     9601 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
     9661 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
     9721 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
     9781 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
     9841 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
     9901 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     9961 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
    10021 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
    10081 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
    10141 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
    10201 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
    10261 tatttgttaa atatgtacta caaacttagt agt
//
