LOCUS       V014965                 8061 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014965
VERSION     V014965
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8061
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             117..750
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    797..922
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1415..1648
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1833..1877
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2026..2067
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2164..2281
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2409..2440
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2473..4122
                     /label="luciferase"
                     /note="firefly luciferase"
     misc_feature    4177..4763
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4783..5379
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             5585..5818
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5975..6199
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6295..6399
                     /label="AmpR promoter"
     CDS             6400..7257
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7431..8019
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
       61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
      121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
      181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
      241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
      301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
      361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
      421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
      481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
      541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
      841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
      901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
      961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca
     2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2281 tcatcgatcg caccagcgtg tggatcgacc acccacgacc acccacgacc acccacgacc
     2341 acccacgacc acccacgacc acccacgatc cgagaacaga tctggcctcg gcggccaagc
     2401 ttagacacta gagggtatat aatggaagct cgacttccag cttggcaatc cggtactgtt
     2461 ggtaaagcca ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatcct
     2521 cttgaggatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt
     2581 cctggaacaa ttgcttttac agatgcacat atcgaggtga acatcacgta cgcggaatac
     2641 ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac
     2701 agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta
     2761 tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac
     2821 agtatgaaca tttcgcagcc taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt
     2881 ttgaacgtgc aaaaaaaatt accaataatc cagaaaatta ttatcatgga ttctaaaacg
     2941 gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt
     3001 aatgaatacg attttgtacc agagtccttt gatcgtgaca aaacaattgc actgataatg
     3061 aactcctctg gatctactgg gttacctaag ggtgtggccc ttccgcatag aactgcctgc
     3121 gtcagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg
     3181 attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg
     3241 atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tttacgatcc
     3301 cttcaggatt acaaaattca aagtgcgttg ctagtaccaa ccctattttc attcttcgcc
     3361 aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc ttctgggggc
     3421 gcacctcttt cgaaagaagt cggggaagcg gttgcaaaac gcttccatct tccagggata
     3481 cgacaaggat atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat
     3541 gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg
     3601 gataccggga aaacgctggg cgttaatcag agaggcgaat tatgtgtcag aggacctatg
     3661 attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga
     3721 tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt catagttgac
     3781 cgcttgaagt ctttaattaa atacaaagga taccaggtgg cccccgctga attggagtcg
     3841 atattgttac aacaccccaa catcttcgac gcgggcgtgg caggtcttcc cgacgatgac
     3901 gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa
     3961 gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt
     4021 gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga
     4081 gagatcctca taaaggccaa gaagggcgga aagtccaaat tgtgactagt gatccgcatg
     4141 catctagggc ggccaattcc gcccctctcc cccccccccc tctccctccc ccccccctaa
     4201 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     4261 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     4321 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     4381 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     4441 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     4501 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     4561 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     4621 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     4681 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     4741 acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga gtacaagccc
     4801 acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg
     4861 ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc
     4921 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     4981 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     5041 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     5101 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     5161 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     5221 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     5281 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga gtgcccgaag
     5341 gaccgcgcga cctggtgcat gacccgcaag cccggtgcct gataataggc ggccgctcga
     5401 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     5461 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     5521 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5581 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     5641 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5701 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5761 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     5821 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     5881 agtgagaggc cttgacatta taatagattt agcaggaatt gaactaggag tggagcacac
     5941 aggcaaagtt ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     6001 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     6061 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     6121 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     6181 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctgggggcg cgcccctcga
     6241 ggccgccatg gtcatagctg tttgacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     6301 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6361 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6421 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6481 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6541 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6601 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6661 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6721 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6781 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6841 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6901 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6961 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7021 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7081 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     7141 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7201 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7261 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7321 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7381 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7441 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7501 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7561 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     7621 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7681 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7741 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7801 ctgagatacc tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7861 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7921 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7981 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8041 ttacggttcc tggccttttg c
//
