LOCUS       V014563                13182 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014563
VERSION     V014563
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13182)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        657..958
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        959..1161
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             1176..1356
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1403..1528
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    2027..2260
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2445..2489
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2638..2679
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2787..2904
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2962..3173
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             3198..7301
                     /label="dCas9"
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             7356..9206
                     /label="p300 core"
                     /note="catalytic histone acetyltransferase core of the
                     human E1A-associated protein p300 (Delvecchio et al.,
                     2013)"
     CDS             9210..9236
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             9255..9275
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             9282..9302
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             9342..9398
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             9399..9794
                     /label="BSD"
                     /note="blasticidin S deaminase"
     CDS             complement(10275..10286)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     LTR             10539..10718
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    10744..10968
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(10994..11012)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(11081..11111)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11126..11147)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(11435..12023)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12197..13054)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13055..13159)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgactagtac
      661 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      721 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      781 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      841 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      901 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      961 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     1021 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     1081 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     1141 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     1201 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     1261 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     1321 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     1381 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     1441 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     1501 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     1561 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     1621 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     1681 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     1741 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     1801 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     1861 gcaagcggcc ggccgcgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1921 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1981 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     2041 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     2101 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     2161 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     2221 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     2281 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     2341 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     2401 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     2461 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     2521 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     2581 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2641 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2701 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     2761 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     2821 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     2881 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     2941 ttagtaccgg gccctacgcg tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     3001 ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta aactgggaaa
     3061 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     3121 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggaccggt
     3181 tctagagcgc tgccaccatg gacaagaagt acagcatcgg cctggccatc ggcaccaact
     3241 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
     3301 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggcgccctg ctgttcgaca
     3361 gcggagaaac agccgaggcc acccggctga agagaaccgc cagaagaaga tacaccagac
     3421 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
     3481 acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc
     3541 ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
     3601 tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg cggctgatct
     3661 atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga
     3721 accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc
     3781 tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca
     3841 gactgagcaa gagcagacgg ctggaaaatc tgatcgccca gctgcccggc gagaagaaga
     3901 atggcctgtt cggcaacctg attgccctga gcctgggcct gacccccaac ttcaagagca
     3961 acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc
     4021 tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga
     4081 acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg
     4141 cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc
     4201 tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga
     4261 gcaagaacgg ctacgccggc tacatcgatg gcggagccag ccaggaagag ttctacaagt
     4321 tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaca
     4381 gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc
     4441 acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg
     4501 acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc
     4561 tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc
     4621 cctggaactt cgaggaagtg gtggacaagg gcgccagcgc ccagagcttc atcgagcgga
     4681 tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt
     4741 acgagtactt caccgtgtac aacgagctga ccaaagtgaa atacgtgacc gagggaatga
     4801 gaaagcccgc cttcctgagc ggcgagcaga aaaaagccat cgtggacctg ctgttcaaga
     4861 ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgct
     4921 tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg ggcacatacc
     4981 acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa aacgaggaca
     5041 ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg atcgaggaac
     5101 ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg aagcggcgga
     5161 gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg gacaagcagt
     5221 ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga aacttcatgc
     5281 agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc caggtgtccg
     5341 gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc gccattaaga
     5401 agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca
     5461 agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag aagggacaga
     5521 agaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg ggcagccaga
     5581 tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg tacctgtact
     5641 acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac cggctgtccg
     5701 actacgatgt ggacgctatc gtgcctcaga gctttctgaa ggacgactcc atcgataaca
     5761 aagtgctgac tcggagcgac aagaaccggg gcaagagcga caacgtgccc tccgaagagg
     5821 tcgtgaagaa gatgaagaac tactggcgcc agctgctgaa tgccaagctg attacccaga
     5881 ggaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg gataaggccg
     5941 gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc
     6001 tggactcccg gatgaacact aagtacgacg agaacgacaa actgatccgg gaagtgaaag
     6061 tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag ttttacaaag
     6121 tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc gtcgtgggaa
     6181 ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc gactacaagg
     6241 tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag gctaccgcca
     6301 agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc ctggccaacg
     6361 gcgagatccg gaagcggcct ctgatcgaga caaacggcga aacaggcgag atcgtgtggg
     6421 ataagggccg ggactttgcc accgtgcgga aagtgctgtc tatgccccaa gtgaatatcg
     6481 tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg cccaagagga
     6541 acagcgacaa gctgatcgcc agaaagaagg actgggaccc taagaagtac ggcggcttcg
     6601 acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca
     6661 agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga agcagcttcg
     6721 agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa aaggacctga
     6781 tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag agaatgctgg
     6841 cctctgccgg cgaactgcag aagggaaacg aactggccct gccctccaaa tatgtgaact
     6901 tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat aatgagcaga
     6961 aacagctgtt tgtggaacag cacaaacact acctggacga gatcatcgag cagatcagcg
     7021 agttctccaa gagagtgatc ctggccgacg ctaatctgga caaggtgctg agcgcctaca
     7081 acaagcacag agacaagcct atcagagagc aggccgagaa tatcatccac ctgtttaccc
     7141 tgaccaatct gggagcccct gccgccttca agtactttga caccaccatc gaccggaaga
     7201 ggtacaccag caccaaagag gtgctggacg ccaccctgat ccaccagagc atcaccggcc
     7261 tgtacgagac acggatcgac ctgtctcagc tgggaggcga caagcgacct gccgccacaa
     7321 agaaggctgg acaggctaag aagaagaaag gatccatttt caaaccagaa gaactacgac
     7381 aggcactgat gccaactttg gaggcacttt accgtcagga tccagaatcc cttccctttc
     7441 gtcaacctgt ggaccctcag cttttaggaa tccctgatta ctttgatatt gtgaagagcc
     7501 ccatggatct ttctaccatt aagaggaagt tagacactgg acagtatcag gagccctggc
     7561 agtatgtcga tgatatttgg cttatgttca ataatgcctg gttatataac cggaaaacat
     7621 cacgggtata caaatactgc tccaagctct ctgaggtctt tgaacaagaa attgacccag
     7681 tgatgcaaag ccttggatac tgttgtggca gaaagttgga gttctctcca cagacactgt
     7741 gttgctacgg caaacagttg tgcacaatac ctcgtgatgc cacttattac agttaccaga
     7801 acaggtatca tttctgtgag aagtgtttca atgagatcca aggggagagc gtttctttgg
     7861 gggatgaccc ttcccagcct caaactacaa taaataaaga acaattttcc aagagaaaaa
     7921 atgacacact ggatcctgaa ctgtttgttg aatgtacaga gtgcggaaga aagatgcatc
     7981 agatctgtgt ccttcaccat gagatcatct ggcctgctgg attcgtctgt gatggctgtt
     8041 taaagaaaag tgcacgaact aggaaagaaa ataagttttc tgctaaaagg ttgccatcta
     8101 ccagacttgg cacctttcta gagaatcgtg tgaatgactt tctgaggcga cagaatcacc
     8161 ctgagtcagg agaggtcact gttagagtag ttcatgcttc tgacaaaacc gtggaagtaa
     8221 aaccaggcat gaaagcaagg tttgtggaca gtggagagat ggcagaatcc tttccatacc
     8281 gaaccaaagc cctctttgcc tttgaagaaa ttgatggtgt tgacctgtgc ttctttggca
     8341 tgcatgttca agagtatggc tctgactgcc ctccacccaa ccagaggaga gtatacatat
     8401 cttacctcga tagtgttcat ttcttccgtc ctaaatgctt gaggactgca gtctatcatg
     8461 aaatcctaat tggatattta gaatatgtca agaaattagg ttacacaaca gggcatattt
     8521 gggcatgtcc accaagtgag ggagatgatt atatcttcca ttgccatcct cctgaccaga
     8581 agatacccaa gcccaagcga ctgcaggaat ggtacaaaaa aatgcttgac aaggctgtat
     8641 cagagcgtat tgtccatgac tacaaggata tttttaaaca agctactgaa gatagattaa
     8701 caagtgcaaa ggaattgcct tatttcgagg gtgatttctg gcccaatgtt ctggaagaaa
     8761 gcattaagga actggaacag gaggaagaag agagaaaacg agaggaaaac accagcaatg
     8821 aaagcacaga tgtgaccaag ggagacagca aaaatgctaa aaagaagaat aataagaaaa
     8881 ccagcaaaaa taagagcagc ctgagtaggg gcaacaagaa gaaacccggg atgcccaatg
     8941 tatctaacga cctctcacag aaactatatg ccaccatgga gaagcataaa gaggtcttct
     9001 ttgtgatccg cctcattgct ggccctgctg ccaactccct gcctcccatt gttgatcctg
     9061 atcctctcat cccctgcgat ctgatggatg gtcgggatgc gtttctcacg ctggcaaggg
     9121 acaagcacct ggagttctct tcactccgaa gagcccagtg gtccaccatg tgcatgctgg
     9181 tggagctgca cacgcagagc caggacgcct atccctatga cgtgcccgat tatgccagcc
     9241 tgggcagcgg ctcccccaag aaaaaacgca aggtggaaga tcctaagaaa aagcggaaag
     9301 tggacggcat tggtagtggg agcaacggca gcagcggatc cgctactaac ttcagcctgc
     9361 tgaagcaggc tggagacgtg gaggagaacc ctggacctat ggccaagcct ttgtctcaag
     9421 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     9481 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     9541 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     9601 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     9661 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     9721 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     9781 atgtgtggga gggctaagcg gccgctcaac ctctggatta caaaatttgt gaaagattga
     9841 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     9901 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     9961 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
    10021 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
    10081 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
    10141 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
    10201 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
    10261 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
    10321 ctctgcggcc tcttccgcgt ctacgccttc gccctcagac gagtcggatc tccctttggg
    10381 ccgcctcccc gcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
    10441 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaaaga agtcaagatc
    10501 tgctttttgc ctgtactggg tctctctggt tagaccagag tctctctggt tagaccagat
    10561 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
    10621 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
    10681 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ttaaacccgc tgatcagcct
    10741 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
    10801 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
    10861 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
    10921 attgggaagt caatagcagg catgctgggg atgcggtggg ctctatgggc ggccgttaat
    10981 taagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
    11041 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    11101 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    11161 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    11221 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    11281 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    11341 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    11401 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    11461 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    11521 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    11581 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    11641 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    11701 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    11761 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    11821 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
    11881 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    11941 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    12001 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    12061 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    12121 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    12181 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    12241 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    12301 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    12361 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    12421 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    12481 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    12541 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    12601 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    12661 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    12721 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    12781 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    12841 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    12901 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    12961 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    13021 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    13081 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    13141 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
