LOCUS       V014575                 8569 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014575
VERSION     V014575
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8569)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             117..750
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    797..922
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1415..1648
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1833..1877
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2026..2067
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2164..2281
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2409..3587
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     sig_peptide     3613..3675
                     /label="Ig-kappa leader"
                     /note="leader sequence from mouse immunoglobulin kappa
                     light chain"
     CDS             3676..3702
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             4483..4629
                     /label="PDGFR-beta TM domain"
                     /note="transmembrane domain from platelet derived growth
                     factor receptor beta"
     misc_feature    4685..5271
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             5291..5887
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             6093..6326
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6483..6707
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6803..6907
                     /label="AmpR promoter"
     CDS             6908..7765
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7939..8527
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
       61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
      121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
      181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
      241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
      301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
      361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
      421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
      481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
      541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
      841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
      901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
      961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca
     2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2281 tcgggtttat tacagggaca gcagagatcc actttggtcg ataagctttg caaagatgga
     2341 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
     2401 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     2461 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     2521 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     2581 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     2641 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     2701 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     2761 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
     2821 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
     2881 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
     2941 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
     3001 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
     3061 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     3121 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     3181 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     3241 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     3301 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     3361 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     3421 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     3481 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     3541 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga attgggatcc
     3601 ccggacgaat tcatggagac agacacactc ctgctatggg tactgctgct ctgggttcca
     3661 ggttccactg gtgactatcc atatgatgtt ccagattatg ctgtgctggc ccaggtgcag
     3721 ctgcagcaga gcggcgccga actggccaga cctggcgcca gcgtgaaaat gtcctgcaag
     3781 gccagcggct acacctttac cagatatacc atgcactggg tgaagcagag acctggacag
     3841 ggcctggagt ggatcggcta catcaacccc tctcggggct acaccaacta caaccagaag
     3901 ttcaaggata aggccacact gacaaccgac aagtcctcct ctaccgccta catgcagctg
     3961 tcctccctga ctagcgagga tagcgccgtg tattactgcg ctaggtatta cgatgaccac
     4021 tactgcctgg actactgggg gcagggcact accgtgaccg tgagcgccgg tggaggtggc
     4081 agtggtggag gtggcagagg cggaggtagt tcacagatcg tgctgaccca gagccccgcc
     4141 atcatgtccg cctcccccgg cgagaaggtg accatgactt gctcagccag ctcctctgtg
     4201 agctatatga attggtacca gcagaaaagc gggaccagtc ccaagaggtg gatctatgat
     4261 acctccaagc tggcttctgg agtgcctgcc cacttcagag gcagcggaag cggcaccagc
     4321 tatagcctga caatctctgg aatggaagcc gaggacgctg ccacatacta ttgccagcag
     4381 tggagcagca acccctttac tttcggcagc gggaccaagc tggagatcaa caggggaggc
     4441 ggcgaccccg aacaaaaact catctcagaa gaggatctga atgctgtggg ccaggacacg
     4501 caggaggtca tcgtggtgcc acactccttg ccctttaagg tggtggtgat ctcagccatc
     4561 ctggccctgg tggtgctcac catcatctcc cttatcatcc tcatcatgct ttggcagaag
     4621 aagccacgtt agactagtga tccgcatgca tctagggcgg ccaattccgc ccctctcccc
     4681 cccccccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     4741 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     4801 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     4861 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     4921 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     4981 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     5041 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     5101 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     5161 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     5221 acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccacaaggag
     5281 acgaccttcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     5341 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     5401 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     5461 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     5521 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     5581 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     5641 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     5701 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     5761 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     5821 cgtcaccgcc gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc
     5881 cggtgcctga taataggcgg ccgctcgaga cctagaaaaa catggagcaa tcacaagtag
     5941 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     6001 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     6061 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     6121 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     6181 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6241 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6301 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     6361 ataacttgca aagaaatgaa tatcagagag tgagaggcct tgacattata atagatttag
     6421 caggaattga actaggagtg gagcacacag gcaaagttct agagctcgct gatcagcctc
     6481 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     6541 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     6601 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     6661 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     6721 aagaaccagc tgggggcgcg cccctcgagg ccgccatggt catagctgtt tgacgtcagg
     6781 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6841 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6901 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     6961 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7021 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7081 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7141 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7201 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7261 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7321 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7381 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7441 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7501 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7561 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7621 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7681 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7741 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7801 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7861 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7921 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7981 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8041 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     8101 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8161 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8221 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8281 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag
     8341 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8401 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8461 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8521 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgc
//
