LOCUS       V014949                 8088 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014949
VERSION     V014949
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             117..750
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    797..922
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1415..1648
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1833..1877
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2026..2067
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2164..2281
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2436..2467
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             2500..4149
                     /label="luciferase"
                     /note="firefly luciferase"
     misc_feature    4204..4790
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4810..5406
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             5612..5845
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6002..6226
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6322..6426
                     /label="AmpR promoter"
     CDS             6427..7284
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7458..8046
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
       61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
      121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
      181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
      241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
      301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
      361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
      421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
      481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
      541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
      841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
      901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
      961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca
     2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2281 tcatcgatcg caccagcgtg tggatcacac cagacattga cgtaagctgc cagatcccat
     2341 tcccgtcata ctctgacgtc tttcagacac cccattgacg tcaatgggag aacgatccga
     2401 gaacagatct ggcctcggcg gccaagctta gacactagag ggtatataat ggaagctcga
     2461 cttccagctt ggcaatccgg tactgttggt aaagccacca tggaagacgc caaaaacata
     2521 aagaaaggcc cggcgccatt ctatcctctt gaggatggaa ccgctggaga gcaactgcat
     2581 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
     2641 gaggtgaaca tcacgtacgc ggaatacttc gaaatgtccg ttcggttggc agaagctatg
     2701 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
     2761 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
     2821 atttataatg aacgtgaatt gctcaacagt atgaacattt cgcagcctac cgtagtgttt
     2881 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaaattacc aataatccag
     2941 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
     3001 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtaccaga gtcctttgat
     3061 cgtgacaaaa caattgcact gataatgaac tcctctggat ctactgggtt acctaagggt
     3121 gtggcccttc cgcatagaac tgcctgcgtc agattctcgc atgccagaga tcctattttt
     3181 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
     3241 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
     3301 tttgaagaag agctgttttt acgatccctt caggattaca aaattcaaag tgcgttgcta
     3361 gtaccaaccc tattttcatt cttcgccaaa agcactctga ttgacaaata cgatttatct
     3421 aatttacacg aaattgcttc tgggggcgca cctctttcga aagaagtcgg ggaagcggtt
     3481 gcaaaacgct tccatcttcc agggatacga caaggatatg ggctcactga gactacatca
     3541 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
     3601 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcagaga
     3661 ggcgaattat gtgtcagagg acctatgatt atgtccggtt atgtaaacaa tccggaagcg
     3721 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
     3781 gaagacgaac acttcttcat agttgaccgc ttgaagtctt taattaaata caaaggatac
     3841 caggtggccc ccgctgaatt ggagtcgata ttgttacaac accccaacat cttcgacgcg
     3901 ggcgtggcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
     3961 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
     4021 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
     4081 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
     4141 tccaaattgt gactagtgat ccgcatgcat ctagggcggc caattccgcc cctctccccc
     4201 ccccccctct ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     4261 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     4321 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     4381 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     4441 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     4501 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     4561 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     4621 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     4681 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca
     4741 cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc cacaaggaga
     4801 cgaccttcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     4861 cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     4921 gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     4981 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     5041 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     5101 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     5161 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     5221 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     5281 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     5341 gtcaccgccg acgtcgagtg cccgaaggac cgcgcgacct ggtgcatgac ccgcaagccc
     5401 ggtgcctgat aataggcggc cgctcgagac ctagaaaaac atggagcaat cacaagtagc
     5461 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     5521 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     5581 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5641 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     5701 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5761 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5821 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     5881 taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattataa tagatttagc
     5941 aggaattgaa ctaggagtgg agcacacagg caaagttcta gagctcgctg atcagcctcg
     6001 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     6061 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     6121 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     6181 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     6241 agaaccagct gggggcgcgc ccctcgaggc cgccatggtc atagctgttt gacgtcaggt
     6301 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     6361 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     6421 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     6481 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     6541 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     6601 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     6661 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     6721 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     6781 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     6841 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     6901 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6961 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7021 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7081 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     7141 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     7201 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     7261 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     7321 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     7381 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7441 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7501 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7561 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     7621 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7681 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7741 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7801 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     7861 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7921 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7981 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8041 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgc
//
