LOCUS       V028511                10911 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028511
VERSION     V028511
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        3..229
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             230..410
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    457..582
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1075..1308
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1803..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    1938..1958
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of attB"
                     /note="core recombination site for the Gateway® BP
                     reaction"
                     /label="attB4"
     enhancer        2019..2322
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2323..2526
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2566..5580
                     /label="EPHA8(NM_020526)"
                     /note="EPHA8(NM_020526)"
                     /gene="EPHA8"
     misc_feature    5626..6214
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     promoter        6245..6744
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6761..7360
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     LTR             7432..7665
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7737..7858
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7898..8033
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(8054..8072)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(8082..8098)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      8240..8695
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8721..8825
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             8826..9686
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      9857..10445
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    10733..10754
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        10769..10799
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10807..10823
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     10831..10847
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        10868..10886
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gttggatccg ccaccatggc ccccgcccgg
     2581 ggccgcctgc cccctgcgct ctgggtcgtc acggccgcgg cggcggcggc cacctgcgtg
     2641 tccgcggcgc gcggcgaagt gaatttgctg gacacgtcga ccatccacgg ggactggggc
     2701 tggctcacgt atccggctca tgggtgggac tccatcaacg aggtggacga gtccttccag
     2761 cccatccaca cgtaccaggt ttgcaacgtc atgagcccca accagaacaa ctggctgcgc
     2821 acgagctggg tcccccgaga cggcgcccgg cgcgtctatg ctgagatcaa gtttaccctg
     2881 cgcgactgca acagcatgcc tggtgtgctg ggcacctgca aggagacctt caacctctac
     2941 tacctggagt cggaccgcga cctgggggcc agcacacaag aaagccagtt cctcaaaatc
     3001 gacaccattg cggccgacga gagcttcaca ggtgccgacc ttggtgtgcg gcgtctcaag
     3061 ctcaacacgg aggtgcgcag tgtgggtccc ctcagcaagc gcggcttcta cctggccttc
     3121 caggacatag gtgcctgcct ggccatcctc tctctccgca tctactataa gaagtgccct
     3181 gccatggtgc gcaatctggc tgccttctcg gaggcagtga cgggggccga ctcgtcctca
     3241 ctggtggagg tgaggggcca gtgcgtgcgg cactcagagg agcgggacac acccaagatg
     3301 tactgcagcg cggagggcga gtggctcgtg cccatcggca aatgcgtgtg cagtgccggc
     3361 tacgaggagc ggcgggatgc ctgtgtggcc tgtgagctgg gcttctacaa gtcagcccct
     3421 ggggaccagc tgtgtgcccg ctgccctccc cacagccact ccgcagctcc agccgcccaa
     3481 gcctgccact gtgacctcag ctactaccgt gcagccctgg acccgccgtc ctcagcctgc
     3541 acccggccac cctcggcacc agtgaacctg atctccagtg tgaatgggac atcagtgact
     3601 ctggagtggg cccctcccct ggacccaggt ggccgcagtg acatcaccta caatgccgtg
     3661 tgccgccgct gcccctgggc actgagccgc tgcgaggcat gtgggagcgg cacccgcttt
     3721 gtgccccagc agacaagcct ggtgcaggcc agcctgctgg tggccaacct gctggcccac
     3781 atgaactact ccttctggat cgaggccgtc aatggcgtgt ccgacctgag ccccgagccc
     3841 cgccgggccg ctgtggtcaa catcaccacg aaccaggcag ccccgtccca ggtggtggtg
     3901 atccgtcaag agcgggcggg gcagaccagc gtctcgctgc tgtggcagga gcccgagcag
     3961 ccgaacggca tcatcctgga gtatgagatc aagtactacg agaaggacaa ggagatgcag
     4021 agctactcca ccctcaaggc cgtcaccacc agagccaccg tctccggcct caagccgggc
     4081 acccgctacg tgttccaggt ccgagcccgc acctcagcag gctgtggccg cttcagccag
     4141 gccatggagg tggagaccgg gaaaccccgg ccccgctatg acaccaggac cattgtctgg
     4201 atctgcctga cgctcatcac gggcctggtg gtgcttctgc tcctgctcat ctgcaagaag
     4261 aggcactgtg gctacagcaa ggccttccag gactcggacg aggagaagat gcactatcag
     4321 aatggacagg cacccccacc tgtcttcctg cctctgcatc accccccggg aaagctccca
     4381 gagccccagt tctatgcgga accccacacc tacgaggagc caggccgggc gggccgcagt
     4441 ttcactcggg agatcgaggc ctctaggatc cacatcgaga aaatcatcgg ctctggagac
     4501 tccggggaag tctgctacgg gaggctgcgg gtgccagggc agcgggatgt gcccgtggcc
     4561 atcaaggccc tcaaagccgg ctacacggag agacagaggc gggacttcct gagcgaggcg
     4621 tccatcatgg ggcaattcga ccatcccaac atcatccgcc tcgagggtgt cgtcacccgt
     4681 ggccgcctgg caatgattgt gactgagtac atggagaacg gctctctgga caccttcctg
     4741 aggacccacg acgggcagtt caccatcatg cagctggtgg gcatgctgag aggagtgggt
     4801 gccggcatgc gctacctctc agacctgggc tatgtccacc gagacctggc cgcccgcaac
     4861 gtcctggttg acagcaacct ggtctgcaag gtgtctgact tcgggctctc acgggtgctg
     4921 gaggacgacc cggatgctgc ctacaccacc acgggcggga agatccccat ccgctggacg
     4981 gccccagagg ccatcgcctt ccgcaccttc tcctcggcca gcgacgtgtg gagcttcggc
     5041 gtggtcatgt gggaggtgct ggcctatggg gagcggccct actggaacat gaccaaccgg
     5101 gatgtcatca gctctgtgga ggaggggtac cgcctgcccg cacccatggg ctgcccccac
     5161 gccctgcacc agctcatgct cgactgttgg cacaaggacc gggcgcagcg gcctcgcttc
     5221 tcccagattg tcagtgtcct cgatgcgctc atccgcagcc ctgagagtct cagggccacc
     5281 gccacagtca gcaggtgccc accccctgcc ttcgtccgga gctgctttga cctccgaggg
     5341 ggcagcggtg gcggtggggg cctcaccgtg ggggactggc tggactccat ccgcatgggc
     5401 cggtaccgag accacttcgc tgcgggcgga tactcctctc tgggcatggt gctacgcatg
     5461 aacgcccagg acgtgcgcgc cctgggcatc accctcatgg gccaccagaa gaagatcctg
     5521 ggcagcattc agaccatgcg ggcccagctg accagcaccc aggggccccg ccggcacctc
     5581 tgactcgagt ctagatgtac aaagtggtga taatcgaatt ccgataatca acctctggat
     5641 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5701 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5761 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5821 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5881 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5941 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     6001 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     6061 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     6121 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     6181 acgagtcgga tctccctttg ggccgcctcc ccgcatcggg aattcccgcg gttcgaattc
     6241 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     6301 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     6361 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     6421 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     6481 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     6541 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     6601 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     6661 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     6721 tcttcctcat ctccgggcct ttcgacctca cgtggccacc atgaccgagt acaagcccac
     6781 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     6841 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     6901 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     6961 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     7021 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     7081 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     7141 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     7201 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     7261 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     7321 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga ggtaccttta agaccaatga
     7381 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7441 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     7501 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7561 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7621 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     7681 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     7741 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7801 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7861 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7921 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7981 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
     8041 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
     8101 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     8161 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     8221 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     8281 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     8341 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8401 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     8461 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     8521 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     8581 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     8641 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     8701 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8761 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8821 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     8881 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     8941 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9001 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9061 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9121 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9181 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9241 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9301 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9361 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9421 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9481 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9541 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9601 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9661 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9721 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9781 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9841 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     9901 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     9961 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    10021 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    10081 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    10141 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    10201 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    10261 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    10321 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    10381 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    10441 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    10501 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    10561 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    10621 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    10681 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    10741 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    10801 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    10861 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
