LOCUS       V045973                10587 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V045973
VERSION     V045973
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        3..229
                     /note="Rous sarcoma virus enhancer/promoter"
                     /label="RSV promoter"
     LTR             230..410
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    457..582
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1075..1308
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1803..1920
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    1938..1958
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of attB"
                     /note="core recombination site for the Gateway® BP
                     reaction"
                     /label="attB4"
     enhancer        2019..2322
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        2323..2526
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2566..5190
                     /label="ENPP3(NM_005021)"
                     /note="ENPP3(NM_005021)"
                     /gene="ENPP3"
     CDS             5197..5262
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     misc_feature    5302..5890
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     promoter        5921..6420
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             6437..7036
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     LTR             7108..7341
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7413..7534
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7574..7709
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     promoter        complement(7730..7748)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     complement(7758..7774)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     rep_origin      7916..8371
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8397..8501
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             8502..9362
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      9533..10121
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    10409..10430
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        10445..10475
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10483..10499
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     10507..10523
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     promoter        10544..10562
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gttggatccg ccaccatgga atctacgttg
     2581 actttagcaa cggaacaacc tgttaagaag aacactctta agaaatataa aatagcttgc
     2641 attgttcttc ttgctttgct ggtgatcatg tcacttggat taggcctggg gcttggactc
     2701 aggaaactgg aaaagcaagg cagctgcagg aagaagtgct ttgatgcatc atttagagga
     2761 ctggagaact gccggtgtga tgtggcatgt aaagaccgag gtgattgctg ctgggatttt
     2821 gaagacacct gtgtggaatc aactcgaata tggatgtgca ataaatttcg ttgtggagag
     2881 accagattag aggccagcct ttgctcttgt tcagatgact gtttgcagag gaaagattgc
     2941 tgtgctgact ataagagtgt ttgccaagga gaaacctcat ggctggaaga aaactgtgac
     3001 acagcccagc agtctcagtg cccagaaggg tttgacctgc caccagttat cttgttttct
     3061 atggatggat ttagagctga atatttatac acatgggata ctttaatgcc aaatatcaat
     3121 aaactgaaaa catgtggaat tcattcaaaa tacatgagag ctatgtatcc taccaaaacc
     3181 ttcccaaatc attacaccat tgtcacgggc ttgtatccag agtcacatgg catcattgac
     3241 aataatatgt atgatgtaaa tctcaacaag aatttttcac tttcttcaaa ggaacaaaat
     3301 aatccagcct ggtggcatgg gcaaccaatg tggctgacag caatgtatca aggtttaaaa
     3361 gccgctacct acttttggcc cggatcagaa gtggctataa atggctcctt tccttccata
     3421 tacatgcctt acaacggaag tgtcccattt gaagagagga tttctacact gttaaaatgg
     3481 ctggacctgc ccaaagctga aagacccagg ttttatacca tgtattttga agaacctgat
     3541 tcctctggac atgcaggtgg accagtcagt gccagagtaa ttaaagcctt acaggtagta
     3601 gatcatgctt ttgggatgtt gatggaaggc ctgaagcagc ggaatttgca caactgtgtc
     3661 aatatcatcc ttctggctga ccatggaatg gaccagactt attgtaacaa gatggaatac
     3721 atgactgatt attttcccag aataaacttc ttctacatgt acgaagggcc tgccccccgc
     3781 atccgagctc ataatatacc tcatgacttt tttagtttta attctgagga aattgttaga
     3841 aacctcagtt gccgaaaacc tgatcagcat ttcaagccct atttgactcc tgatttgcca
     3901 aagcgactgc actatgccaa gaacgtcaga atcgacaaag ttcatctctt tgtggatcaa
     3961 cagtggctgg ctgttaggag taaatcaaat acaaattgtg gaggaggcaa ccatggttat
     4021 aacaatgagt ttaggagcat ggaggctatc tttctggcac atggacccag ttttaaagag
     4081 aagactgaag ttgaaccatt tgaaaatatt gaagtctata acctaatgtg tgatcttcta
     4141 cgcattcaac cagcaccaaa caatggaacc catggtagtt taaaccatct tctgaaggtg
     4201 cctttttatg agccatccca tgcagaggag gtgtcaaagt tttctgtttg tggctttgct
     4261 aatccattgc ccacagagtc tcttgactgt ttctgccctc acctacaaaa tagtactcag
     4321 ctggaacaag tgaatcagat gctaaatctc acccaagaag aaataacagc aacagtgaaa
     4381 gtaaatttgc catttgggag gcctagggta ctgcagaaga acgtggacca ctgtctcctt
     4441 taccacaggg aatatgtcag tggatttgga aaagctatga ggatgcccat gtggagttca
     4501 tacacagtcc cccagttggg agacacatcg cctctgcctc ccactgtccc agactgtctg
     4561 cgggctgatg tcagggttcc tccttctgag agccaaaaat gttccttcta tttagcagac
     4621 aagaatatca cccacggctt cctctatcct cctgccagca atagaacatc agatagccaa
     4681 tatgatgctt taattactag caatttggta cctatgtatg aagaattcag aaaaatgtgg
     4741 gactacttcc acagtgttct tcttataaaa catgccacag aaagaaatgg agtaaatgtg
     4801 gttagtggac caatatttga ttataattat gatggccatt ttgatgctcc agatgaaatt
     4861 accaaacatt tagccaacac tgatgttccc atcccaacac actactttgt ggtgctgacc
     4921 agttgtaaaa acaagagcca cacaccggaa aactgccctg ggtggctgga tgtcctaccc
     4981 tttatcatcc ctcaccgacc taccaacgtg gagagctgtc ctgaaggtaa accagaagct
     5041 ctttgggttg aagaaagatt tacagctcac attgcccggg tccgtgatgt agaacttctc
     5101 actgggcttg acttctatca ggataaagtg cagcctgtct ctgaaatttt gcaactaaag
     5161 acatatttac caacatttga aaccactatt ctcgaggact acaaagacca tgacggtgat
     5221 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagtctag atgtacaaag
     5281 tggtgataat cgaattccga taatcaacct ctggattaca aaatttgtga aagattgact
     5341 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5401 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5461 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5521 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5581 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5641 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     5701 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     5761 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     5821 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     5881 gcctccccgc atcgggaatt cccgcggttc gaattctacc gggtagggga ggcgcttttc
     5941 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     6001 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     6061 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     6121 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     6181 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     6241 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     6301 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     6361 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     6421 acctcacgtg gccaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     6481 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     6541 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     6601 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     6661 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     6721 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     6781 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     6841 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     6901 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     6961 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     7021 caagcccggt gcctgaggta cctttaagac caatgactta caaggcagct gtagatctta
     7081 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     7141 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7201 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7261 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7321 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     7381 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     7441 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     7501 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     7561 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7621 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7681 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
     7741 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     7801 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     7861 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     7921 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     7981 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8041 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8101 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     8161 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     8221 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     8281 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8341 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     8401 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     8461 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     8521 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     8581 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     8641 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     8701 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     8761 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     8821 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     8881 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     8941 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     9001 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     9061 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     9121 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     9181 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     9241 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     9301 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     9361 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     9421 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     9481 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9541 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9601 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9661 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9721 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9781 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     9841 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     9901 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     9961 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
    10021 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
    10081 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10141 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10201 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    10261 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
    10321 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
    10381 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    10441 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    10501 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
    10561 gggaacaaaa gctggagctg caagctt
//
