LOCUS       V014729                 8270 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014729
VERSION     V014729
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             117..750
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    797..922
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1415..1648
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1833..1877
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2026..2067
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2164..2281
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             2682..4331
                     /label="luciferase"
                     /note="firefly luciferase"
     misc_feature    4386..4972
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4992..5588
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             5794..6027
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6184..6408
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6504..6608
                     /label="AmpR promoter"
     CDS             6609..7466
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7640..8228
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
       61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
      121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
      181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
      241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
      301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
      361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
      421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
      481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
      541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
      841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
      901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
      961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca
     2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2281 tcgggtttat tacagggaca gcagagatcc actttggatc gataagcttt gcaaagatgg
     2341 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
     2401 gtgggaattg gatccgggga ctttccgctt ggggactttc cgctggggac tttccgctgg
     2461 ggactttccg ctggggactt tccgaattca gcttgaataa aatgaatatt agaagctgtt
     2521 agaataagag aaaatgacag aggaaaactg aaagggagaa ctgaaagtgg gaaattcctc
     2581 tgaggcagaa aggaccatcc cttataaata gcacaggcca tgaaggaaga tcattctcac
     2641 tgcagccttt gacagccttt gcctcatctt gaagcttcac catggaagac gccaaaaaca
     2701 taaagaaagg cccggcgcca ttctatcctc ttgaggatgg aaccgctgga gagcaactgc
     2761 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
     2821 tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc cgttcggttg gcagaagcta
     2881 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc
     2941 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg
     3001 acatttataa tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct accgtagtgt
     3061 ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta ccaataatcc
     3121 agaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt
     3181 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtacca gagtcctttg
     3241 atcgtgacaa aacaattgca ctgataatga actcctctgg atctactggg ttacctaagg
     3301 gtgtggccct tccgcataga actgcctgcg tcagattctc gcatgccaga gatcctattt
     3361 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt
     3421 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata
     3481 gatttgaaga agagctgttt ttacgatccc ttcaggatta caaaattcaa agtgcgttgc
     3541 tagtaccaac cctattttca ttcttcgcca aaagcactct gattgacaaa tacgatttat
     3601 ctaatttaca cgaaattgct tctgggggcg cacctctttc gaaagaagtc ggggaagcgg
     3661 ttgcaaaacg cttccatctt ccagggatac gacaaggata tgggctcact gagactacat
     3721 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc
     3781 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaga
     3841 gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg ttatgtaaac aatccggaag
     3901 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg
     3961 acgaagacga acacttcttc atagttgacc gcttgaagtc tttaattaaa tacaaaggat
     4021 accaggtggc ccccgctgaa ttggagtcga tattgttaca acaccccaac atcttcgacg
     4081 cgggcgtggc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt
     4141 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa
     4201 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta
     4261 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa
     4321 agtccaaatt gtgactagtg atccgcatgc atctagggcg gccaattccg cccctctccc
     4381 ccccccccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     4441 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     4501 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     4561 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     4621 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     4681 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     4741 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     4801 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     4861 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     4921 cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccacaagga
     4981 gacgaccttc catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     5041 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     5101 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     5161 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     5221 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     5281 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     5341 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     5401 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     5461 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     5521 ccgtcaccgc cgacgtcgag tgcccgaagg accgcgcgac ctggtgcatg acccgcaagc
     5581 ccggtgcctg ataataggcg gccgctcgag acctagaaaa acatggagca atcacaagta
     5641 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     5701 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     5761 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5821 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     5881 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     5941 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6001 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     6061 tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattat aatagattta
     6121 gcaggaattg aactaggagt ggagcacaca ggcaaagttc tagagctcgc tgatcagcct
     6181 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     6241 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     6301 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     6361 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     6421 aaagaaccag ctgggggcgc gcccctcgag gccgccatgg tcatagctgt ttgacgtcag
     6481 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6541 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6601 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6661 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6721 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6781 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6841 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6901 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6961 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7021 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7081 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7141 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7201 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7261 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7321 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7381 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7441 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7501 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7561 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7621 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7681 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7741 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     7801 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7861 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7921 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7981 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa
     8041 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8101 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8161 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8221 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
//
