LOCUS       V014559                 7338 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014559
VERSION     V014559
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7338)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             117..750
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    797..922
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1415..1648
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1833..1877
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2026..2067
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2164..2281
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             2682..3398
                     /label="ECFP"
                     /note="enhanced CFP"
     misc_feature    3454..4040
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4060..4656
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             4862..5095
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5252..5476
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5572..5676
                     /label="AmpR promoter"
     CDS             5677..6534
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6708..7296
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
       61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
      121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
      181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
      241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
      301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
      361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
      421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
      481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
      541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
      841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
      901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
      961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca
     2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2281 tcgggtttat tacagggaca gcagagatcc actttggatc gataagcttt gcaaagatgg
     2341 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
     2401 gtgggaattg gatccgggga ctttccgctt ggggactttc cgctggggac tttccgctgg
     2461 ggactttccg ctggggactt tccgaattca gcttgaataa aatgaatatt agaagctgtt
     2521 agaataagag aaaatgacag aggaaaactg aaagggagaa ctgaaagtgg gaaattcctc
     2581 tgaggcagaa aggaccatcc cttataaata gcacaggcca tgaaggaaga tcattctcac
     2641 tgcagccttt gacagccttt gcctcatctt gaagcttcac catggtgagc aagggcgagg
     2701 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     2761 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     2821 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     2881 ggggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2941 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     3001 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     3061 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     3121 tcagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
     3181 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     3241 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     3301 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     3361 ccgccgggat cactctcggc atggacgagc tgtacaagta aactagtgat ccgcatgcat
     3421 ctagggcggc caattccgcc cctctccccc ccccccctct ccctcccccc cccctaacgt
     3481 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     3541 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     3601 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     3661 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     3721 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     3781 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     3841 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     3901 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     3961 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     4021 atgataagct tgccacaacc cacaaggaga cgaccttcca tgaccgagta caagcccacg
     4081 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc
     4141 gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc
     4201 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     4261 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     4321 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     4381 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     4441 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     4501 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     4561 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgagtg cccgaaggac
     4621 cgcgcgacct ggtgcatgac ccgcaagccc ggtgcctgat aataggcggc cgctcgagac
     4681 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     4741 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     4801 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     4861 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     4921 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4981 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5041 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     5101 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     5161 gagaggcctt gacattataa tagatttagc aggaattgaa ctaggagtgg agcacacagg
     5221 caaagttcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5281 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     5341 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     5401 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     5461 gcggtgggct ctatggcttc tgaggcggaa agaaccagct gggggcgcgc ccctcgaggc
     5521 cgccatggtc atagctgttt gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5581 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5641 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5701 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5761 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5821 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5881 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5941 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     6001 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     6061 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     6121 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     6181 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     6241 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     6301 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     6361 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6421 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6481 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6541 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6601 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6661 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6721 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6781 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6841 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6901 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6961 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     7021 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7081 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7141 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7201 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7261 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7321 cggttcctgg ccttttgc
//
