LOCUS       40924_28826        4553 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Eukaryotic luciferase expression vector pLUC+, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Altschmied J, Duschl J.
  TITLE     Set of optimized luciferase reporter gene plasmids compatible with 
            widely used CAT vectors
  JOURNAL   BioTechniques 23 (3), 436-438 (1997)
  PUBMED    9298214
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Altschmied J, Duschl J.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-1997) Physiological Chemistry I/Biocenter, 
            University of Wuerzburg, Am Hubland, Wuerzburg 97074, Germany
REFERENCE   3  (bases 1 to 4553)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4553)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1997"; volume: "23"; issue: "3"; pages: 
            "436-438"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-SEP-1997) Physiological Chemistry I/Biocenter, University of 
            Wuerzburg, Am Hubland, Wuerzburg 97074, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..41
                     /label=polylinker
                     /note="polylinker"
     CDS             80..1729
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1773..1894)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    1988..2004
                     /label=polylinker
                     /note="polylinker"
     primer_bind     complement(2026..2042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2050..2066)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2074..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2119..2140)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2428..3016)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3190..4047)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4048..4152)
                     /label=AmpR promoter
     polyA_signal    4179..4313
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    4416..4550
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccagat ctcgagatct gagcttggca
       61 ttccggtact gttggtaaaa tggaagacgc caaaaacata aagaaaggcc cggcgccatt
      121 ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
      181 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc
      241 tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
      301 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
      361 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
      421 gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca
      481 aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc
      541 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
      601 cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact
      661 gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac
      721 tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
      781 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
      841 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgtttct
      901 gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt
      961 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
     1021 tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc
     1081 aggtatcagg caaggatatg ggctcactga gactacatca gctattctga ttacacccga
     1141 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
     1201 ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg
     1261 tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
     1321 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
     1381 cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt
     1441 ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtcttcccga
     1501 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
     1561 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
     1621 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
     1681 aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt aattctagag
     1741 tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
     1801 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     1861 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     1921 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     1981 tggtaaaatc gatccccggg taccgagctc gaattcgtaa tcatggtcat agctgtttcc
     2041 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     2101 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     2161 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     2221 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     2281 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     2341 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     2401 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     2461 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     2521 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     2581 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
     2641 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     2701 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     2761 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     2821 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     2881 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     2941 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3001 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3061 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     3121 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     3181 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     3241 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     3301 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     3361 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     3421 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     3481 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     3541 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     3601 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     3661 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3721 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3781 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3841 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3901 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3961 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4021 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4081 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     4141 aggggttccg cgcacatttc cccgaaaagt gccacctgaa cttgtttatt gcagcttata
     4201 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     4261 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcataatca
     4321 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     4381 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     4441 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4501 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tcg
//
