LOCUS       V004923                 6400 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004923
VERSION     V004923
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Tian L.
  TITLE     Elucidation of the ethanol tolerance mechanism of Clostridium
            thermocellum by metabolome analysis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Tian L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2017) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 6400)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6400)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-JUL-2017) Thayer School of Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1002
                     /label="repB"
                     /note="RepB replication protein"
     CDS             1281..2288
                     /codon_start=1
                     /gene="Gapdh"
                     /product="Gapdh"
                     /label="Gapdh"
                     /note="derived from Thermoanaerobacterium saccharolyticum"
                     /protein_id="AWT04834.1"
                     /translation="MAVKVGINGFGRIGRNFFRAALKKNVDLDIVAFNDLTDAKTLAHL
                     LKYDSTFGQFEGEVIAKEDSLVVNGKEIKILKETDPAKLPWKELGVDIVIESTGRFTNK
                     EDAVKHIEAGAKKVIISAPAKNEDITIVMGVNEDKYDPNAHHVISNASCTTNCLAPFAK
                     VLHNKFGIKRGLMTTVHSYTNDQRILDLPHKDLRRARSAAMSIIPTTTGAAKAVALVLP
                     ELKGKLNGFAMRVPTPDVSVVDLVAELEKSVTVEEVNAALKEAAENELKGILGYTDEPL
                     VSMDFKGDSRSSIVDGLSTMVMEGNMVKVVSWYDNEWGYSNRVVDLAKYVADRL"
     gene            1281..2288
                     /gene="Gapdh"
                     /label="Gapdh"
     CDS             2430..3077
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(3161..4018)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4019..4110)
                     /label="AmpR promoter"
     rep_origin      4869..5091
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             5139..6086
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
ORIGIN
        1 atgggggttt cttttaatat tatgtgtcct aatagtagca tttattcaga tgaaaaatca
       61 agggttttag tggacaagac aaaaagtgga aaagtgagac catggagaga aaagaaaatc
      121 gctaatgttg attactttga acttctgcat attcttgaat ttaaaaaggc tgaaagagta
      181 aaagattgtg ctgaaatatt agagtataaa caaaatcgtg aaacaggcga aagaaagttg
      241 tatcgagtgt ggttttgtaa atccaggctt tgtccaatgt gcaactggag gagagcaatg
      301 aaacatggca ttcagtcaca aaaggttgtt gctgaagtta ttaaacaaaa gccaacagtt
      361 cgttggttgt ttctcacatt aacagttaaa aatgtttatg atggcgaaga attaaataag
      421 agtttgtcag atatggctca aggatttcgc cgaatgatgc aatataaaaa aattaataaa
      481 aatcttgttg gttttatgcg tgcaacggaa gtgacaataa ataataaaga taattcttat
      541 aatcagcaca tgcatgtatt ggtatgtgtg gaaccaactt attttaagaa tacagaaaac
      601 tacgtgaatc aaaaacaatg gattcaattt tggaaaaagg caatgaaatt agactatgat
      661 ccaaatgtaa aagttcaaat gattcgaccg aaaaataaat ataaatcgga tatacaatcg
      721 gcaattgacg aaactgcaaa atatcctgta aaggatacgg attttatgac cgatgatgaa
      781 gaaaagaatt tgaaacgttt gtctgatttg gaggaaggtt tacaccgtaa aaggttaatc
      841 tcctatggtg gtttgttaaa agaaatacat aaaaaattaa accttgatga cacagaagaa
      901 ggcgatttga ttcatacaga tgatgacgaa aaagccgatg aagatggatt ttctattatt
      961 gcaatgtgga attgggaacg gaaaaattat tttattaaag agtagttcaa cccggggatc
     1021 ctctagagtc gagataaaca aaggacggtt cagggcttct gctcatccta ctctgcattg
     1081 taaaaaggta ggatgaattt ttatttttaa tcttattgaa aaaaattttt gaaaatcggt
     1141 tttattaaaa aaaagtgggt atatttataa tagtcaattg attggttaaa aaaatttaaa
     1201 taagcaaaca gaataataac aaaagtaagg aggaatttgt tagaagacgt acaaaaagaa
     1261 aacagcagga ggttcgagta atggcagtaa aagtaggtat taatggcttt ggtaggatag
     1321 gcagaaactt ttttagagca gcattaaaga aaaatgtaga tcttgatatt gttgcattca
     1381 atgatcttac tgatgcaaaa acattagcac atctattaaa atacgattca acattcggtc
     1441 aattcgaagg cgaagtaatc gcaaaagaag attcacttgt tgtaaatggc aaggaaataa
     1501 aaatattaaa agaaacagat cctgcaaaat taccttggaa ggaattaggt gttgacatag
     1561 ttatcgaatc aacaggtaga tttacaaaca aggaagatgc agtaaaacac atcgaagctg
     1621 gagcaaagaa agtaataatt tctgcacctg ctaaaaatga agatataaca atcgttatgg
     1681 gtgttaacga ggacaaatac gatccaaatg cacatcacgt tatatcaaat gcttcatgca
     1741 cgacaaactg cttagcacca tttgcaaaag ttttacataa caaatttgga atcaagagag
     1801 gtttaatgac aactgttcac tcatacacaa acgatcagag gatactggac cttcctcata
     1861 aagatttaag aagagcaaga tcagcagcaa tgtcaattat tccgacaaca acaggagctg
     1921 caaaggctgt tgcacttgta ttgcctgaac taaaaggaaa attaaacggc tttgctatga
     1981 gagttccaac accagacgta tctgttgtcg accttgttgc agagcttgaa aagagcgtaa
     2041 ctgtagaaga agtaaatgca gcattgaaag aagcggcaga aaacgagctt aaaggcattc
     2101 tcggatatac agatgagcca ttagtatcaa tggacttcaa aggcgattca agatcatcaa
     2161 ttgttgatgg cttgtcaacg atggttatgg aaggcaatat ggtaaaagtt gtttcatggt
     2221 atgacaacga atggggttat tcaaacaggg ttgttgacct tgctaagtat gtagcagata
     2281 gactgtaagg catgcaagct ttaatagaaa taattttgtt taactttaca aacgggattg
     2341 acttttaaaa aaggattgat tctaatgaag aaagcagaca agtaagcctc ctaaattcac
     2401 tttagataaa aatttaggag gcatatcaaa tgaactttaa taaaattgat ttagacaatt
     2461 ggaagagaaa agagatattt aatcattatt tgaaccaaca aacgactttt agtataacca
     2521 cagaaattga tattagtgtt ttataccgaa acataaaaca agaaggatat aaattttacc
     2581 ctgcatttat tttcttagtg acaagggtga taaactcaaa tacagctttt agaactggtt
     2641 acaatagcga cggagagtta ggttattggg ataagttaga gccactttat acaatttttg
     2701 atggtgtatc taaaacattc tctggtattt ggactcctgt aaagaatgac ttcaaagagt
     2761 tttatgattt atacctttct gatgtagaga aatataatgg ttcggggaaa ttgtttccca
     2821 aaacacctat acctgaaaat gctttttctc tttctattat tccatggact tcatttactg
     2881 ggtttaactt aaatatcaat aataatagta attaccttct acccattatt acagcaggaa
     2941 aattcattaa taaaggtaat tcaatatatt taccgctatc tttacaggta catcattctg
     3001 tttgtgatgg ttatcatgca ggattgttta tgaactctat tcaggaattg tcagataggc
     3061 ctaatgactg gcttttataa tatgagataa tgccgactgt actttttaca gtcggttttc
     3121 taatgtcact agggctcgcc tttgggaagt ttgaaggtta ccaatgctta atcagtgagg
     3181 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3241 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3301 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3361 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3421 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     3481 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3541 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3601 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3661 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3721 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     3781 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3841 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3901 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3961 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4021 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4081 tatttgaatg tatttagaaa aataaacaaa agagtttgag gaaaacttaa gaaaacggga
     4141 aactatttcc tgttgagaat gtgaaattaa atgataaaaa gtgatataat agggtcaggc
     4201 gtacagaagc ctgacctttt tgcaaaattg ttttattggc gcggcgtata tatacagcat
     4261 atatacaatg taaatttata caatcaaatt gttgatacaa tcgaattatg cggctaaaaa
     4321 tttgctcatg agcctagaaa taaccagcct aacttcgatc attggaccgc tgatcgtcac
     4381 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc
     4441 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac
     4501 ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccgcagac ccgccataaa
     4561 acgccctgag aagcccgtga cgggcttttc ttgtattatg ggtagtttcc ttgcatgaat
     4621 ccataaaagg cgcctgtagt gccatttacc cccattcact gccagagccg tgagcgcagc
     4681 gaactgaatg tcacgaaaaa gacagcgact caggtgcctg atggtcggag acaaaaggaa
     4741 tattcagcga tttgcccgag cttgcgaggg tgctacttaa gcctttaggg ttttaaggtc
     4801 tgttttgtag aggagcaaac agcgtttgcg acatcctttt gtaatactgc ggaactgact
     4861 aaagtagtga gttatacaca gggctgggat ctattctttt tatctttttt tattctttct
     4921 ttattctata aattataacc acttgaatat aaacaaaaaa aacacacaaa ggtctagcgg
     4981 aatttacaga gggtctagca gaatttacaa gttttccagc aaaggtctag cagaatttac
     5041 agatacccac aactcaaagg aaaaggacta gtaattatca ttgactagcc catctcaatt
     5101 ggtatagtga ttaaaatcac ctagaccaat tgagatgtat gtctgaatta gttgttttca
     5161 aagcaaatga actagcgatt agtcgctatg acttaacgga gcatgaaacc aagctaattt
     5221 tatgctgtgt ggcactactc aaccccacga ttgaaaaccc tacaaggaaa gaacggacgg
     5281 tatcgttcac ttataaccaa tacgctcaga tgatgaacat cagtagggaa aatgcttatg
     5341 gtgtattagc taaagcaacc agagagctga tgacgagaac tgtggaaatc aggaatcctt
     5401 tggttaaagg ctttgagatt ttccagtgga caaactatgc caagttctca agcgaaaaat
     5461 tagaattagt ttttagtgaa gagatattgc cttatctttt ccagttaaaa aaattcataa
     5521 aatataatct ggaacatgtt aagtcttttg aaaacaaata ctctatgagg atttatgagt
     5581 ggttattaaa agaactaaca caaaagaaaa ctcacaaggc aaatatagag attagccttg
     5641 atgaatttaa gttcatgtta atgcttgaaa ataactacca tgagtttaaa aggcttaacc
     5701 aatgggtttt gaaaccaata agtaaagatt taaacactta cagcaatatg aaattggtgg
     5761 ttgataagcg aggccgcccg actgatacgt tgattttcca agttgaacta gatagacaaa
     5821 tggatctcgt aaccgaactt gagaacaacc agataaaaat gaatggtgac aaaataccaa
     5881 caaccattac atcagattcc tacctacata acggactaag aaaaacacta cacgatgctt
     5941 taactgcaaa aattcagctc accagttttg aggcaaaatt tttgagtgac atgcaaagta
     6001 agtatgatct caatggttcg ttctcatggc tcacgcaaaa acaacgaacc acactagaga
     6061 acatactggc taaatacgga aggatctgag gttcttatgg ctcttgtatc tatcagtgaa
     6121 gcatcaagac taacaaacaa aagtagaaca actgttcacc gttacatatc aaagggaaaa
     6181 ctgtcaatga gtgctttttt tgcgttttga gcgtagcgaa aaacgagttc tttctattct
     6241 tgatacatat agaaataacg tcatttttat tttagttgct gaaaggtgcg ttgaagtgtt
     6301 ggtatgtatg tgttttaaag tattgaaaac ccttaaaatt ggttgcacag aaaaacccca
     6361 tctgttaaag ttataagtga ccaaacaaat aactaaatag
//
