LOCUS       Exported                6258 bp DNA     circular SYN 06-AUG-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Zhang XY, La Russa VF, Reiser J
  TITLE     Transduction of bone-marrow-derived mesenchymal stem cells by using 
            lentivirus vectors pseudotyped with modified RD114 envelope 
            glycoproteins.
  JOURNAL   J Virol. 2004 Feb . 78(3):1219-29.
  PUBMED    14722277
REFERENCE   2  (bases 1 to 6258)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6258)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6258)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2004 Feb . 78(3):1219-29."
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6258
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(28..6258,1..27)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             209..743
                     /label=HIV-1 3' LTR
     CDS             1220..2812
                     /codon_start=1
                     /label=RD114 Env
                     /translation="MKLPTGMVILCSLIIVRAGFDDPRKAIALVQKQHGKPCECSGGQV
                     SEAPPNSIQQVTCPGKTAYLMTNQKWKCRVTPKNLTPSGGELQNCPCNTFQDSMHSSCY
                     TEYRQCRANNKTYYTATLLKIRSGSLNEVQILQNPNQLLQSPCRGSINQPVCWSATAPI
                     HISDGGGPLDTKRVWTVQKRLEQIHKAMHPELQYHPLALPKVRDDLSLDARTFDILNTT
                     FRLLQMSNFSLAQDCWLCLKLGTPTPLAIPTPSLTYSLADSLANASCQIIPPLLVQPMQ
                     FSNSSCLSSPFINDTEQIDLGAVTFTNCTSVANVSSPLCALNGSVFLCGNNMAYTYLPQ
                     NWTGLCVQASLLPDIDIIPGDEPVPIPAIDHYIHRPKRAVQFIPLLAGLGITAAFTTGA
                     TGLGVSVTQYTKLSHQLISDVQVLSGTIQDLQDQVDSLAEVVLQNRRGLDLLTAEQGGI
                     CLALQEKCCFYANKSGIVRNKIRTLQEELQKRRESLASNPLWTGLQGFLPYLLPLLGPL
                     LTLLLILTIGPCVF"
     CDS             2813..2914
                     /codon_start=1
                     /label=ENV from the MLV 4070A
                     /note="cytoplasmic tail domain derived from the MLV 4070A"
                     /translation="NRLVQFVKDRISVVQALVLTQQYHQLKPIEYEP"
     polyA_signal    3044..3165
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3172..3627)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3654..3758
                     /label=AmpR promoter
     promoter        3760..4117
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4152..4943
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5134..5153)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5178..5225
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5554..6142
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttattaccg gactcagatc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
       61 acagcagcta acaatgctgc ttgtgcctgg ctagaagcac aagaggagga agaggtgggt
      121 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
      181 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca aagaagacaa
      241 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
      301 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
      361 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
      421 gtgagcctgc atggaatgga tgaccctgag agagaagtgt tagagtggag gtttgacagc
      481 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
      541 catcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
      601 gggactgggg agtggcgagc cctcagatgc tgcatataag cagctgcttt ttgcctgtac
      661 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
      721 actgcttaag cctcaataaa gctgatcccc cgggctgcag gaatttatga aatcctttat
      781 gggggacccc cccctttgtc aaccttgctc aattccttct ccccctccga tcctaagact
      841 gatttacaag cccgactaaa agggctgcaa gcggtgcagg cccaaatctg gacacccctg
      901 gccgaattgt accggccagg acatccacaa actagccacc catttcaggt gggagactcc
      961 gtgtacgtcc ggcggcaccg ctctcaagga ttggagcctc gttggaaggg accttacatc
     1021 gtcctgctga ccacgcccac cgccataaag gttgacggga tcgccgcctg gattcacgca
     1081 tcgcacgcca aggcagcccc aaaaacccct ggaccagaaa ctcccaaaac ctggaagctc
     1141 cgccgttcgg agaaccctct taagataaga ctctcccgtg tctgactgct aatccacctt
     1201 gtccctgtac taacccaaaa tgaaactccc aacaggaatg gtcattttat gtagcctaat
     1261 aatagttcgg gcagggtttg acgacccccg caaggctatc gcattagtac aaaaacaaca
     1321 tggtaaacca tgcgaatgca gcggagggca ggtatccgag gccccaccga actccatcca
     1381 acaggtaact tgcccaggca agacggccta cttaatgacc aaccaaaaat ggaaatgcag
     1441 agtcactcca aaaaatctca cccctagcgg gggagaactc cagaactgcc cctgtaacac
     1501 tttccaggac tcgatgcaca gttcttgtta tactgaatac cggcaatgca gggcgaataa
     1561 taagacatac tacacggcca ccttgcttaa aatacggtct gggagcctca acgaggtaca
     1621 gatattacaa aaccccaatc agctcctaca gtccccttgt aggggctcta taaatcagcc
     1681 cgtttgctgg agtgccacag cccccatcca tatctccgat ggtggaggac ccctcgatac
     1741 taagagagtg tggacagtcc aaaaaaggct agaacaaatt cataaggcta tgcatcctga
     1801 acttcaatac caccccttag ccctgcccaa agtcagagat gaccttagcc ttgatgcacg
     1861 gacttttgat atcctgaata ccacttttag gttactccag atgtccaatt ttagccttgc
     1921 ccaagattgt tggctctgtt taaaactagg tacccctacc cctcttgcga tacccactcc
     1981 ctctttaacc tactccctag cagactccct agcgaatgcc tcctgtcaga ttatacctcc
     2041 cctcttggtt caaccgatgc agttctccaa ctcgtcctgt ttatcttccc ctttcattaa
     2101 cgatacggaa caaatagact taggtgcagt cacctttact aactgcacct ctgtagccaa
     2161 tgtcagtagt cctttatgtg ccctaaacgg gtcagtcttc ctctgtggaa ataacatggc
     2221 atacacctat ttaccccaaa actggacagg actttgcgtc caagcctccc tcctccccga
     2281 cattgacatc atcccggggg atgagccagt ccccattcct gccattgatc attatataca
     2341 tagacctaaa cgagctgtac agttcatccc tttactagct ggactgggaa tcaccgcagc
     2401 attcaccacc ggagctacag gcctaggtgt ctccgtcacc cagtatacaa aattatccca
     2461 tcagttaata tctgatgtcc aagtcttatc cggtaccata caagatttac aagaccaggt
     2521 agactcgtta gctgaagtag ttctccaaaa taggagggga ctggacctac taacggcaga
     2581 acaaggagga atttgtttag ccttacaaga aaaatgctgt ttttatgcta acaagtcagg
     2641 aattgtgaga aacaaaataa gaaccctaca agaagaatta caaaaacgca gggaaagcct
     2701 ggcatccaac cctctctgga ccgggctgca gggctttctt ccgtacctcc tacctctcct
     2761 gggaccccta ctcaccctcc tactcatact aaccattggg ccatgcgttt tcaatcgatt
     2821 ggtccaattt gttaaagaca ggatctcagt ggtccaggct ctggttttga ctcagcaata
     2881 tcaccagcta aaacccatag agtacgagcc atgaagatcc accggatcta gataactgat
     2941 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     3001 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     3061 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3121 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa
     3181 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     3241 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     3301 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     3361 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     3421 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     3481 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     3541 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     3601 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3661 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     3721 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     3781 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3841 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     3901 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3961 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4021 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4081 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     4141 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     4201 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     4261 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     4321 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     4381 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     4441 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     4501 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     4561 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     4621 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     4681 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     4741 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     4801 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     4861 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     4921 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     4981 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     5041 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     5101 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     5161 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     5221 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     5281 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     5341 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     5401 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5461 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5521 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5581 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5641 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5701 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5761 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5821 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5881 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5941 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6001 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6061 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6121 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6181 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6241 tattaccgcc atgcatta
//
