LOCUS       62056_14450        5299 bp DNA     circular SYN 13-MAY-2019
DEFINITION  Cloning vector pLTG1, complete sequence.
ACCESSION   MK190413
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Li L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2018) Key Laboratory of Synthetic Biology, 
            Institute of Plant Physiology and Ecology, 300 Feng Lin Road, 
            Shanghai, Shanghai 200032, China
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (15-NOV-2018) Key Laboratory of Synthetic Biology, Institute of 
            Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 
            200032, China"
FEATURES             Location/Qualifiers
     source          1..5299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1207..1218
                     /codon_start=1
                     /label=WELQut site
                     /note="WELQut protease recognition and cleavage site"
                     /translation="WELQ"
     primer_bind     complement(2201..2217)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2225..2241)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2249..2279)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2294..2315)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2603..3191)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             3395..4195
                     /codon_start=1
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     CDS             complement(4632..5000)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(5033..5142)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 tccgcgaacg gctcatcagg tacggcgtgc cgacggtcga ggcgggcggc gtatgggcca
       61 accaggaagg gccggtaagc gtcacggcac gcgccgactg agagacgttt ccgcaggtca
      121 accccgttcc agcccaacag tgttagtctt tgctcttacc cagttgggcg ggatagcctg
      181 cccggcatga gcgtgaaggt tgaaggcatg gtcattctgg caggcggcta cgaccgacag
      241 tcggcggaac gggagaacag ttcgaccgct tcaccggcca cccagcgcgc cgcgaaccgg
      301 gggaaggctg aggcgctggc gaaggagtac gcgcgcgacg gcgtcgaggt gaagtggctg
      361 ggtcacttca gcgaagcgcc cggcacgtcg gcattcacgg gcgtcgaccg gccggagttc
      421 aaccggattt tggacatgtg ccggaaccgg gaaatgaaca tgatcattgt tcattacatt
      481 tcgcgcctca gccgcgaaga gccgctggac attattccgg tcgtcacgga attgctccgg
      541 ctgggcgtga ccattgtcag cgtgaacgaa ggcacattcc gccccggcga aatgatggac
      601 cttattcacc tgatcatgcg ccttcaggct tcgcatgatg agtcgaagaa caagagcgtc
      661 gccgtgtcga acgctaagga attggcgaag cggctgggcg gacacacggg gtcgacgccg
      721 tacggattcg acacggtcga ggaaatggtt ccgaacccgg aagacggcgg aaagctggtt
      781 gccattcgcc gactggtgcc cagcgcgcac acctgggaag gcgcacacgg cagcgaaggg
      841 gcggtaatcc gctgggcgtg gcaggagatc aagacgcacc gcgatacgcc attcaagggt
      901 ggcggagccg ggtcgtttca ccctgggtcg ctgaacgggc tttgtgagcg gctgtaccgc
      961 gacaaggtgc ctacgcgcgg cacgctggtc ggtaagaagc gcgccggttc cgattgggac
     1021 cccggcgttt tgaagcgcgt actcagcgac ccgcgcattg ccgggtatca agctgacatc
     1081 gcatacaagg tgcgcgccga cggttcgcgg ggcggcttca gccattacaa gatcaggcgc
     1141 gacccggtca ccatggagcc gctgaccctg cccggcttcg agccgtacat tcccccggcg
     1201 gaatggtggg aacttcagga gtggcttcag ggtcgaggac gcgggaaggg tcagtaccgg
     1261 gggcaatcgc tcctgtcggc aatggacgtc ctttactgct acggctccgg ccagctcgac
     1321 ccggagacgg gttacagcaa cgggtcgacc atggcgggca acgtccgcga aggtgatcaa
     1381 gctcacaagt cgtcgtacgc gtgcaagtgc ccccgccggg ttcatgacgg gtcgtcatgc
     1441 tcgatcacga tgcacaacct tgacccgtac atcgtcggcg cgatcttcgc gcgcatcacg
     1501 gccttcgacc ctgccgaccc tgacgacctc gaaggcgaca cggcagcgct catgtacgaa
     1561 gccgcacggc gctggggagc gacgcacgaa cgcccggagt tgaagggtca gcgctccgaa
     1621 ctgatggcac agcgcgcgga cgccgtgaag gcgctcgaag agctttacga agacaagcgg
     1681 aacggcggct accggtccgc catgggacgg cgcgcgtttc tcgaagagga agccgcgctg
     1741 acgctccgca tggaaggggc cgaagaacgg cttcgtcagc tcgacgccgc cgactccccc
     1801 gtgctgccga tcggcgaatg gctgggcgac cggggcagcg acccgacggg accgggttcg
     1861 tggtgggcgc tagcgcccct tgaagaccgt cgggcgttcg tccggctctt cgtggaccgg
     1921 atcgaggtga tcaagcttcc gaagggcgtt cagcggcccg gacgggttcc cccgatcgcc
     1981 gaccgtgtgc gtatccactg ggcgaagccg aaggtcgagg aagagacgga gccggagacg
     2041 ctgaacgggt tcacagcggc ggcgtgacgg cggcaccagc gcaacgggaa ggggcttcgg
     2101 ccccttttct cgtgcccggc gtcggttcgt tgccctaagc aactgttcct agcgtcacgt
     2161 caggatccgc ggccgcgcgc gatatcgaat tcgtaatcat gtcatagctg tttcctgtgt
     2221 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2281 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2341 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2401 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2461 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2521 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     2581 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2641 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2701 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2761 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2821 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2881 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2941 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3001 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3061 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3121 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     3181 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     3241 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     3301 tggttcatgt gcagctccat cagcaaaagg ggatgataag tttatcacca ccgactattt
     3361 gcaacagtgc cgttgatcgt gctatgatcg actgatgtca tcagcggtgg agtgcaatgt
     3421 cgtgcaatac gaatggcgaa aagccgagct catcggtcag cttctcaacc ttggggttac
     3481 ccccggcggt gtgctgctgg tccacagctc cttccgtagc gtccggcccc tcgaagatgg
     3541 gccacttgga ctgatcgagg ccctgcgtgc tgcgctgggt ccgggaggga cgctcgtcat
     3601 gccctcgtgg tcaggtctgg acgacgagcc gttcgatcct gccacgtcgc ccgttacacc
     3661 ggaccttgga gttgtctctg acacattctg gcgcctgcca aatgtaaagc gcagcgccca
     3721 tccatttgcc tttgcggcag cggggccaca ggcagagcag atcatctctg atccattgcc
     3781 cctgccacct cactcgcctg caagcccggt cgcccgtgtc catgaactcg atgggcaggt
     3841 acttctcctc ggcgtgggac acgatgccaa cacgacgctg catcttgccg agttgatggc
     3901 aaaggttccc tatggggtgc cgagacactg caccattctt caggatggca agttggtacg
     3961 cgtcgattat ctcgagaatg accactgctg tgagcgcttt gccttggcgg acaggtggct
     4021 caaggagaag agccttcaga aggaaggtcc agtcggtcat gcctttgctc ggttgatccg
     4081 ctcccgcgac attgtggcga cagccctggg tcaactgggc cgagatccgt tgatcttcct
     4141 gcatccgcca gaggcgggat gcgaagaatg cgatgccgct cgccagtcga ttggctgagc
     4201 tcatgagcgg agaacgagat gacgttggag gggcaaggtc gcgctgattg ctggggcaac
     4261 acgtggagcg gatcggggat tgtctttctt cagctcgctg atgatatgct gacgctcaat
     4321 gccgtttggc ctccgactaa cgaaaatccc gcatttggac ggctgatccg attggcacgg
     4381 cggacggcga atggcggagc agacgctcgt ccgggggcaa tgagatatga aaaagcctga
     4441 actcaccgcg acgtatcggg ccctggccag ctagctagag tcgacctgca ggtccccggg
     4501 gatcggtctt gccttgctcg tcggtgatgt acttcaccag ctccgcgaag tcgctcttct
     4561 tgatggagcg catggggacg tgcttggcaa tcacgcgcac cccccggccg ttttagcggc
     4621 taaaaaagtc atggctctgc cctcgggcgg accacgccca tcatgacctt gccaagctcg
     4681 tcctgcttct cttcgatctt cgccagcagg gcgaggatcg tggcatcacc gaaccgcgcc
     4741 gtgcgcgggt cgtcggtgag ccagagtttc agcaggccgc ccaggcggcc caggtcgcca
     4801 ttgatgcggg ccagctcgcg gacgtgctca tagtccacga cgcccgtgat tttgtagccc
     4861 tggccgacgg ccagcaggta ggccgacagg ctcatgccgg ccgccgccgc cttttcctca
     4921 atcgctcttc gttcgtctgg aaggcagtac accttgatag gtgggctgcc cttcctggtt
     4981 ggcttggttt catcagccat ccgcttgccc tcatctgtta cgccggcggt agccggccag
     5041 cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag
     5101 gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata
     5161 caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat
     5221 ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaagatc
     5281 cgtcgacctg caggcatgc
//
