LOCUS       V004929                 5393 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004929
VERSION     V004929
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Tian L, Lo J, Shao X, Zheng T, Olson DG, Lynd LR.
  TITLE     Identification of a ferredoxin:NAD+ oxidoreductase enzyme in
            Thermoanaerobacterium saccharolyticum and its role in ethanol
            formation
  JOURNAL   Appl. Environ. Microbiol. (2016) In press
   PUBMED   27694237
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Tian L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAY-2016) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Dr, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 5393)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5393)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-MAY-2016) Thayer School of Engineering, Dartmouth College, 14
            Engineering Dr, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(66..654)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(828..1685)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(1686..1790)
                     /label="AmpR promoter"
     misc_recomb     1902..2371
     misc_recomb     2371..2372
     CDS             3377..4138
                     /codon_start=1
                     /product="HTK"
                     /label="HTK"
                     /note="KanR"
                     /protein_id="AOU74533.1"
                     /translation="MKGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQTD
                     GPYSDIEMMCVLSTEGVEFSYEWTTGEWKAEVNFYSEEILLDYASRVEPDWPLTHGRFF
                     SILPIYDPGGYFEKVYQTAKSVEAQKFHDAICALIVEELFEYAGKWRNIRVQGPTTFLP
                     SLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQPDLPPGYVQLCQLVMSGQLSDPEK
                     LLESLENFWNGVQEWAERHGYIVDVSKRIPF"
     CDS             4162..4740
                     /gene="tdk"
                     /label="Thymidine kinase"
                     /note="Thymidine kinase from Thermoanaerobacter
                     pseudethanolicus (strain ATCC 33223 / 39E). Accession#:
                     B0K7G9"
     misc_recomb     4822..5283
ORIGIN
        1 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
       61 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      121 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      181 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      241 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      301 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      361 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      421 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      481 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
      541 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      601 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      661 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
      721 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
      781 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
      841 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
      901 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
      961 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1021 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1081 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1141 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1201 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1261 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1321 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1381 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1441 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     1501 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     1561 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     1621 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     1681 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     1741 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     1801 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     1861 aggcgtatca cgagatgcat cagggcaaag aaaaaagtgg tggactgttt attcttgtga
     1921 aaacttccaa tgttggttct ggtacaattc aaaatttaaa aactatgaat ggcactgtgt
     1981 atgaaaatgt ggcatacatg gttgataaga tttcaaaact ggccaaaggc agtttaggat
     2041 atagttctat aggtgcagtt gttggagcta cgtataaaga ggaggccaaa atactgagaa
     2101 aaataatgcc atctgctatc tttttggtgc ctggatatgg agcacagggt gctactgcag
     2161 aagacgtcat taattgtttt gacgaaaaca acttaggtgc tatagttaac tcatcgagaa
     2221 aagttatctt tgcttataaa agtcaatact ggaaagatgt ttattctgaa tatgagtatg
     2281 ctcaagctgc acgtgctgaa gttcttctga tgatggggat gattaataat gcgtttttaa
     2341 aaagaagata tgttgcgtgt tgaggtgaaa tgtaaggatg gtcctgtatt ttggtcaagg
     2401 gaggtagtat tttgaatctg aaagtcaata taggaaacct tgaacttaaa aatccagttc
     2461 ttgtggcatc tggcacattt ggctttggga gagagtattc acattatatc gatttagatg
     2521 atttaggagg aataatggta aaggggctta cattgtatcc aaaagaagga aatcctccac
     2581 cgcgtatata cgaaacgcca tcaggaattt taaatagcgt tgggctgcaa aatccaggtg
     2641 tcgatgcatt cataaaagat gaattgccat ttttaaatag gtttaatact aaagtgatcg
     2701 caaatatagc aggagagaca atagaagaat ttgtatcatt agcccaaaaa cttaatatag
     2761 acggtgtaga tgcattagag atcaatgttt catgcccaaa tgtaaaagaa ggagggatgt
     2821 cgtttggagt agatcccaac acgtaaaacc cagcgaacca tttgaggtga taggtaagat
     2881 tataccgagg tatgaaaacg agaattggac ctttacagaa ttactctatg aagcgccata
     2941 tttaaaaagc taccaagacg aagaggatga agaggatgag gaggcagatt gccttgaata
     3001 tattgacaat actgataaga taatatatct tttatataga agatatcgcc gtatgtaagg
     3061 atttcagggg gcaaggcata ggcagcgcgc ttatcaatat atctatagaa tgggcaaagc
     3121 ataaaaactt gcatggacta atgcttgaaa cccaggacaa taaccttata gcttgtaaat
     3181 tctatcataa ttgtggtttc aaaatcggct ccgtcgatac tatgttatac gccaactttc
     3241 aaaacaactt tgaaaaagct gttttctggt atttaaggtt ttagaatgca aggaacagtg
     3301 aattggagtt cgtcttgtta taattagctt cttggggtat ctttaaatac tgtagaaaag
     3361 aggaaggaaa taataaatga aaggaccaat aataatgact agagaagaaa gaatgaagat
     3421 tgttcatgaa attaaggaac gaatattgga taaatatggg gatgatgtta aggcaattgg
     3481 tgtttatggc tctcttggtc gtcagactga tgggccctat tcggatattg agatgatgtg
     3541 tgttctgtca acagagggag tagagttcag ctatgaatgg acaaccggtg agtggaaggc
     3601 ggaagtgaat ttttatagcg aagagattct actagattat gcatctcggg tggaaccgga
     3661 ttggccgctt acacatggtc gatttttctc tattttgccg atttatgatc caggtggata
     3721 ctttgagaaa gtgtaccaaa ctgctaaatc ggtagaagcc caaaagttcc acgatgcgat
     3781 ctgtgccctt atcgtagaag agctgtttga atatgcaggc aaatggcgta atattcgtgt
     3841 gcaaggaccg acaacatttc taccatcctt gactgtacag gtggcaatgg caggtgccat
     3901 gttgattggt ctgcatcatc gcatctgtta tacgacgagc gcttcggtct taactgaagc
     3961 agttaagcaa ccagatcttc ctccaggtta tgtccaactg tgccagctcg taatgtctgg
     4021 tcaactttcc gaccctgaga aacttctgga atcgctagag aatttctgga atggggttca
     4081 ggagtgggcg gaacgacacg gatatatagt ggatgtgtca aaacgcatac cattttgatg
     4141 acattaagaa aggtggtttt tatgatatac ggttctttgg atcatggttt tatagaggct
     4201 atagttggtc ctatgtttag cggtaaaagt gaagaactca taagaaggat taaaagagcc
     4261 caaattgcca aacaaaaagt acaagtcttt aaaccggcta ttgacgatag atattctatt
     4321 gacaaggtgg tatctcacaa tggcactaat ataaatgcta ttagtgtagt aaaggcttct
     4381 gaaataattg agcttttaga agaagacaca gaggttatcg caattgatga aattcagttt
     4441 tttgaccatt cgattgtgga tgttgtaaga gaaattgcag atctaggcaa aagagtcatt
     4501 tgtgctggct tagatatgga ctttaggggg gaaccttttg gccctactcc tgatataatg
     4561 gcaattgcgg agtctgtaga taaactgacg gctatatgtg tgaaatgtgg caatcctgct
     4621 actcgcactc aaaggcttat caatgggaaa cctgcaaaat acgatgaccc aatcatttta
     4681 gtaggggccc atgaaaccta tgaagcaagg tgtcgaaaat gccatgaagt tcctcgtaca
     4741 tagtagtacc tagatttaga tgtctaaaaa gctttttaga catctaatct tttctgaagt
     4801 acatccgcaa ctgtccatac tcggataaaa ggaaaaggca cttataatct ttcgaaggtt
     4861 aaagctttag atacattaga ggtaactggt cctcatggtc atggatttga tatgtttgat
     4921 aaattcaaca atattctcat cgttggtggt ggcataggga taccaccact actatttctt
     4981 tcaaaaaaat tgaaatcgaa aaatctaaag attgctcttg gttttaaaag cgatgtattt
     5041 cttgtggatg aatttaaaaa atatggtgat gtcgttattg cgactgaaga tgggaatacc
     5101 ggtattaaag ggtatgttac cgatgcaatg aaagatgaaa taaaggatat tgatatgatt
     5161 tatggatgtg gcccaatgca aatgcttaaa tctgtaaaag acatggctga aaagtacgat
     5221 gtgccttgtc aaatctctat tgaagaacat atgggttgtg gaattggtgc gtgtatggta
     5281 tgtcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     5341 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca gga
//
