LOCUS       62056_14445        5190 bp DNA     circular SYN 13-MAY-2019
DEFINITION  Cloning vector pLSV1, complete sequence.
ACCESSION   MK190412
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Li L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2018) Key Laboratory of Synthetic Biology, 
            Institute of Plant Physiology and Ecology, 300 Feng Lin Road, 
            Shanghai, Shanghai 200032, China
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (15-NOV-2018) Key Laboratory of Synthetic Biology, Institute of 
            Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 
            200032, China"
FEATURES             Location/Qualifiers
     source          1..5190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(224..1867)
                     /codon_start=1
                     /transl_table=11
                     /product="SV1 integrase"
                     /label=SV1 integrase
                     /protein_id="QCO69839.1"
                     /translation="MKRDLPSTFRGSRTPGEPWLGYIRVSTWREEKISPELQQSAIESW
                     AARTGRRIVDWIVDLDATGRNFKRKIMGGIQRVEGREAVGIAVWKFSRFGRNDLGIAIN
                     LARLEQAGGDLASATEEVDARTAVGRFNRAILFDLAVFESDRAGEQWKETHAHRRALKL
                     PATGRQRFGYVWHPRRVPDLTAPGGFRLQEERYERHPEFAPVAAELYERKLAGQGFSQL
                     AYWLNDELLIPTTRGNRWGTNTVQRYLDSGFAAGLLRVHDPECRCKLGQDHFSACKENR
                     WLWLPGAQPALIVPEQWKEYGAHREQTRKTPPRARRASYPTSGIMRHGHCRGTAVARSG
                     RDGKGGFVPGHVFVCFNRRNKGKSACEPGLYVRRDEVEAEVLKWLADTVADDIDNAPAL
                     PAQRTAPGTAPDPRARLVEERTRTEAELAKIEGALDRLVTDYALDPDKYPADTFGRVRD
                     QLLGKKGDIIKHLKSLSEVEVAPTREEFRPLIVGLLQEWDILHTTEKNAILRRLLRRLV
                     IHNRKSDQGAQWSVVRSFEFHPVWEPDPWS"
     primer_bind     complement(2092..2108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2116..2132)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2140..2170)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2185..2206)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2494..3082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             3286..4086
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     CDS             complement(4523..4891)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(4924..5033)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 agacgcggtg ggccgcacat ggaacggccc cggtacctga ggtaggtacc ggggccgttc
       61 ttcgcgctcc gatgtggtcc tttagatcca ctgacgtggg tcagtgtctc taaaggactc
      121 gcgagcatcg ttccccctct ccctgcactg aggtgcggtt ttcagagggt ggcagcaggc
      181 ggagaaacaa tcaagcgcgc ctgcatggtt tgcgctccag cggtcaagac caggggtcgg
      241 gctcccacac gggatgaaac tcgaagctgc gcacgacact ccactgtgcg ccctgatcgc
      301 tcttacggtt gtggatgacc agacggcgca gcaggcggcg caggatggcg ttcttctcgg
      361 tggtgtgcag gatgtcccac tcctgcaaga gcccgacgat cagggggcgg aactcctccc
      421 gcgtgggcgc gacttcaacc tcgctgagcg acttcaagtg cttgatgatg tcgcccttct
      481 taccgagtag ctggtcgcgt acgcgcccga acgtgtccgc cgggtacttg tccgggtcca
      541 gcgcgtaatc cgtgacgaga cggtcgagtg caccctcgat cttggccaac tcggcttccg
      601 tgcgggtgcg ctcctcgacc aggcgggccc gcgggtccgg cgcggtgccc ggcgcggtgc
      661 gctgagcggg aagggccggc gcgttgtcga tgtcgtcggc gaccgtgtcg gcgagccact
      721 tcaacacctc ggcctcgact tcgtcgcggc gcacgtagag gccgggctca caggccgact
      781 tccccttgtt cctgcggttg aagcacacga acacgtggcc cggaacgaat ccgcccttcc
      841 cgtcgcggcc ggatcgcgca acggccgtcc cgcggcagtg tccgtgccgc atgatgccgc
      901 tggtcgggta tgaggcccgg cgggcgcgcg ggggcgtctt gcgcgtctgc tctctgtgcg
      961 ccccgtactc cttccactgc tcgggaacga tgagcgccgg ctgtgccccg gggagccaga
     1021 gccaccggtt ttctttgcac gcagagaagt ggtcctgtcc cagcttgcac cggcactccg
     1081 ggtcgtggac gcgtagcagg ccggcggcga atccggagtc gaggtagcgc tgaacggtgt
     1141 tggtgcccca gcggttgccc cgtgtggtgg ggatgagtag ttcgtcgttc agccagtagg
     1201 cgagctggga gaacccctgt ccggcgagct ttcgctcgta gagttcggcc gccacggggg
     1261 cgaactctgg gtgccgttcg tagcgctcct cttgcaggcg gaacccgccc ggcgcggtga
     1321 ggtcgggtac gcgcctcggg tgccacacgt agccgaaccg ctgacgcccg gtggcgggga
     1381 gtttcagggc ccgacggtgg gcgtgcgtct ccttccactg ttcgccggcc cgatccgact
     1441 cgaatacggc gaggtcgaac agaatcgcgc ggttgaagcg tccgacggcc gtgcgggcgt
     1501 cgacttcttc cgtggcggac gcgaggtctc cgccggcctg ttcgaggcgg gcgaggttga
     1561 tagcgatgcc caggtcgttc cggccgaagc ggctgaactt ccatacggcg attccgacgg
     1621 cctcgcggcc ctcgacgcgc tggatgccgc ccatgatctt ccgcttgaag ttgcggcccg
     1681 tagcgtcgag gtcaacgatc cagtcgacga tccgacgtcc cgttcgggcg gcccatgact
     1741 cgatcgcgga ttgctgtagc tccgggctga tcttctcctc gcgccatgtg ctgaccctga
     1801 tgtagccgag ccacggctcg cccggcgtgc gggagccgcg gaacgtgctt ggtaggtctc
     1861 gtttcatatg tggatcctac caaccggcac gattgtgccc acaacagcat cgcggtgcca
     1921 cgtgtggacc gcgtcggtca gatcctcccc gcacctctcg ccagccgtca agatcgaccg
     1981 cgtgcacctg cgatcgccga tcaaccgcga ctagcatcgg gcgcaagccg ccactcgaac
     2041 ggacactcgc atgagatccg cggccgcgcg cgatatcgaa ttcgtaatca tgtcatagct
     2101 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2161 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     2221 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2281 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2341 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2401 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2461 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2521 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2581 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2641 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2701 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2761 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     2821 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2881 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2941 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3001 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3061 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3121 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3181 ctagatcctt ttggttcatg tgcagctcca tcagcaaaag gggatgataa gtttatcacc
     3241 accgactatt tgcaacagtg ccgttgatcg tgctatgatc gactgatgtc atcagcggtg
     3301 gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc tcatcggtca gcttctcaac
     3361 cttggggtta cccccggcgg tgtgctgctg gtccacagct ccttccgtag cgtccggccc
     3421 ctcgaagatg ggccacttgg actgatcgag gccctgcgtg ctgcgctggg tccgggaggg
     3481 acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc tgccacgtcg
     3541 cccgttacac cggaccttgg agttgtctct gacacattct ggcgcctgcc aaatgtaaag
     3601 cgcagcgccc atccatttgc ctttgcggca gcggggccac aggcagagca gatcatctct
     3661 gatccattgc ccctgccacc tcactcgcct gcaagcccgg tcgcccgtgt ccatgaactc
     3721 gatgggcagg tacttctcct cggcgtggga cacgatgcca acacgacgct gcatcttgcc
     3781 gagttgatgg caaaggttcc ctatggggtg ccgagacact gcaccattct tcaggatggc
     3841 aagttggtac gcgtcgatta tctcgagaat gaccactgct gtgagcgctt tgccttggcg
     3901 gacaggtggc tcaaggagaa gagccttcag aaggaaggtc cagtcggtca tgcctttgct
     3961 cggttgatcc gctcccgcga cattgtggcg acagccctgg gtcaactggg ccgagatccg
     4021 ttgatcttcc tgcatccgcc agaggcggga tgcgaagaat gcgatgccgc tcgccagtcg
     4081 attggctgag ctcatgagcg gagaacgaga tgacgttgga ggggcaaggt cgcgctgatt
     4141 gctggggcaa cacgtggagc ggatcgggga ttgtctttct tcagctcgct gatgatatgc
     4201 tgacgctcaa tgccgtttgg cctccgacta acgaaaatcc cgcatttgga cggctgatcc
     4261 gattggcacg gcggacggcg aatggcggag cagacgctcg tccgggggca atgagatatg
     4321 aaaaagcctg aactcaccgc gacgtatcgg gccctggcca gctagctaga gtcgacctgc
     4381 aggtccccgg ggatcggtct tgccttgctc gtcggtgatg tacttcacca gctccgcgaa
     4441 gtcgctcttc ttgatggagc gcatggggac gtgcttggca atcacgcgca ccccccggcc
     4501 gttttagcgg ctaaaaaagt catggctctg ccctcgggcg gaccacgccc atcatgacct
     4561 tgccaagctc gtcctgcttc tcttcgatct tcgccagcag ggcgaggatc gtggcatcac
     4621 cgaaccgcgc cgtgcgcggg tcgtcggtga gccagagttt cagcaggccg cccaggcggc
     4681 ccaggtcgcc attgatgcgg gccagctcgc ggacgtgctc atagtccacg acgcccgtga
     4741 ttttgtagcc ctggccgacg gccagcaggt aggccgacag gctcatgccg gccgccgccg
     4801 ccttttcctc aatcgctctt cgttcgtctg gaaggcagta caccttgata ggtgggctgc
     4861 ccttcctggt tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg
     4921 tagccggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc gaataaggga
     4981 cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg cccggctgac
     5041 gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct gtatatcgtg
     5101 cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag ggttatgcag
     5161 cggaaaagat ccgtcgacct gcaggcatgc
//
