LOCUS       40924_28781        5034 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLSU-11, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Lee S.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2010) Plant Pathology and Crop Physiology, 
            Louisiana State University, 302 Life Science Bldg, Baton Rouge, LA 
            70820, USA
REFERENCE   2  (bases 1 to 5034)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5034)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (11-OCT-2010) Plant Pathology and Crop Physiology, Louisiana State 
            University, 302 Life Science Bldg, Baton Rouge, LA 70820, USA"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             95..1282
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     rep_origin      1484..2072
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_binding    2145..2167
                     /label=dnaA binding site
                     /bound_moiety="dnaA"
     CDS             2314..2940
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3372..4442
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4511..4705
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4872..4895
                     /label=T-DNA left border repeat
                     /note="T-DNA left border repeat"
     misc_feature    4968..5029
                     /label=T-DNA right border repeat
                     /note="T-DNA right border repeat"
ORIGIN
        1 cctagctctc atgtttgaca gcttatcatc gataagctag gtttaatgcg gtagtttatc
       61 acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt
      121 catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc cggtactgcc
      181 gggcctcttg cgggatattg tccattccga cagcatcgcc agtcactatg gcgtgctgct
      241 tgcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac tgtccgaccg
      301 ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg actacgcgat
      361 catggcgacc acacccgtcc tgtggattct ctacgccgga cgcatcgtgg ccggcatcac
      421 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
      481 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
      541 ggccggggga ctgttgggcg ccatctcctt gcacgcacca ttccttgcgg cggcggtgct
      601 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
      661 tcggccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
      721 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
      781 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg
      841 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc
      901 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct
      961 gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct
     1021 tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga
     1081 tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat
     1141 cattggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt
     1201 ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc
     1261 atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc
     1321 actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct
     1381 tggcagaaca tatccatcgc gtccgccatc tccagctgcc gcacgctcgg tcgttcgaat
     1441 gcggcgagcg gtatcagctc actcgcctag gcaattgatc ttcttgaaat cctttttttc
     1501 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1561 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1621 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1681 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1741 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1801 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1861 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1921 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1981 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     2041 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2101 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2161 tggataaccg tattaccgcc tttgagtgat ctctgaagaa accgagcgcc gccgtctaaa
     2221 aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg
     2281 atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag
     2341 aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc
     2401 ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc
     2461 gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac
     2521 gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac
     2581 ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct
     2641 tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg
     2701 gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc
     2761 ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg
     2821 cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc
     2881 gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt
     2941 tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc
     3001 cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga
     3061 agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac
     3121 gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa
     3181 tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat
     3241 caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg
     3301 ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc
     3361 gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg
     3421 gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg
     3481 cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc
     3541 ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac
     3601 cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg
     3661 acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg
     3721 agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg
     3781 attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg
     3841 aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt
     3901 tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt
     3961 taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga
     4021 cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc
     4081 ggccggagta catcgagatc gagctagttg attggatgta ccgcgagatc acagaaggca
     4141 agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc
     4201 gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca
     4261 agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc
     4321 gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg
     4381 ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt
     4441 cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag
     4501 gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga
     4561 acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg
     4621 atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc
     4681 ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa
     4741 aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg
     4801 cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac
     4861 aagctaggat tggcaggata tattcagttg taaatgatat cgcatgcaag cttccatggc
     4921 tcgagggtac cgaattcgga tccctgcaga gtacttctag agagctctgg caggatatat
     4981 gcggttgtaa ttcattttta ttgtctaaat ttctgtattt gtttgtttgc aatt
//
