LOCUS       40924_28776        4566 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pLSU-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Lee S, Su G, Lasserre E, Aghazadeh MA, Murai N.
  TITLE     Small high-yielding binary Ti vectors pLSU with co-directional 
            replicons for Agrobacterium tumefaciens-mediated transformation of 
            higher plants
  JOURNAL   Plant Sci. 187, 49-58 (2012)
  PUBMED    22404832
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Lee S.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2010) Plant Pathology and Crop Physiology, 
            Louisiana State University, 302 Life Science Bldg, Baton Rouge, LA 
            70820, USA
REFERENCE   3  (bases 1 to 4566)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4566)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            187, 49-58 (2012)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-OCT-2010) Plant Pathology and Crop Physiology, Louisiana State 
            University, 302 Life Science Bldg, Baton Rouge, LA 70820, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             111..923
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1012..1600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_binding    1673..1695
                     /label=dnaA binding site
                     /bound_moiety="dnaA"
     CDS             1846..2472
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2904..3974
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4043..4237
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4404..4427
                     /label=T-DNA left border repeat
                     /note="T-DNA left border repeat"
     misc_feature    4500..4561
                     /label=T-DNA right border repeat
                     /note="T-DNA right border repeat"
ORIGIN
        1 agatccacgt tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
       61 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
      121 ttcaacggga aacgtcttgt tcgaggccgc gattaaattc caacatggat gctgatttat
      181 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc taccgattgt
      241 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
      301 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
      361 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atcccaggga
      421 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
      481 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
      541 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
      601 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
      661 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
      721 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
      781 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
      841 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
      901 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga
      961 gcattacgct gacttgacgg gacggcggct ttgttgaatc aattgatctt cttgaaatcc
     1021 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1081 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1141 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1201 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1261 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1321 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1381 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1441 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1501 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1561 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1621 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1681 tgattctgtg gataaccgta ttaccgcctt tgagtgatct gatcctgaag aaaccgagcg
     1741 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     1801 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     1861 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
     1921 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     1981 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     2041 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     2101 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     2161 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     2221 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     2281 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     2341 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     2401 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     2461 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     2521 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     2581 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     2641 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
     2701 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     2761 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     2821 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga
     2881 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
     2941 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     3001 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     3061 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     3121 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     3181 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     3241 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     3301 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     3361 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     3421 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     3481 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     3541 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     3601 gcgaaaccgg gcggccggag tacatcgaga tcgagctagt tgattggatg taccgcgaga
     3661 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     3721 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     3781 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     3841 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     3901 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     3961 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     4021 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     4081 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     4141 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     4201 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     4261 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     4321 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctgc
     4381 tagcgcgcgg acaagctagg attggcagga tatattcagt tgtaaatgat atcgcatgca
     4441 agcttccatg gctcgagggt accgaattcg gatccctgca gagtacttct agagagctct
     4501 ggcaggatat atgcggttgt aattcatttt tattgtctaa atttctgtat ttgtttgttt
     4561 gcaatt
//
