LOCUS       62056_14430        9381 bp DNA     circular SYN 13-MAY-2019
DEFINITION  Cloning vector pLSRB2, complete sequence.
ACCESSION   MK176927
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9381)
  AUTHORS   Li L.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2018) Key Laboratory of Synthetic Biology, 
            Institute of Plant Physiology and Ecology, 300 Feng Lin Road, 
            Shanghai, Shanghai 200032, China
REFERENCE   2  (bases 1 to 9381)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2018) Key Laboratory of Synthetic Biology, Institute of 
            Plant Physiology and Ecology, 300 Feng Lin Road, Shanghai, Shanghai 
            200032, China"
FEATURES             Location/Qualifiers
     source          1..9381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(137..153)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(161..177)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(185..215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(230..251)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(539..1127)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1635..2426)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     oriT            complement(3013..3122)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             3469..5112
                     /codon_start=1
                     /transl_table=11
                     /product="SV1 integrase"
                     /label=SV1 integrase
                     /protein_id="QCO69795.1"
                     /translation="MKRDLPSTFRGSRTPGEPWLGYIRVSTWREEKISPELQQSAIESW
                     AARTGRRIVDWIVDLDATGRNFKRKIMGGIQRVEGREAVGIAVWKFSRFGRNDLGIAIN
                     LARLEQAGGDLASATEEVDARTAVGRFNRAILFDLAVFESDRAGEQWKETHAHRRALKL
                     PATGRQRFGYVWHPRRVPDLTAPGGFRLQEERYERHPEFAPVAAELYERKLAGQGFSQL
                     AYWLNDELLIPTTRGNRWGTNTVQRYLDSGFAAGLLRVHDPECRCKLGQDHFSACKENR
                     WLWLPGAQPALIVPEQWKEYGAHREQTRKTPPRARRASYPTSGIMRHGHCRGTAVARSG
                     RDGKGGFVPGHVFVCFNRRNKGKSACEPGLYVRRDEVEAEVLKWLADTVADDIDNAPAL
                     PAQRTAPGTAPDPRARLVEERTRTEAELAKIEGALDRLVTDYALDPDKYPADTFGRVRD
                     QLLGKKGDIIKHLKSLSEVEVAPTREEFRPLIVGLLQEWDILHTTEKNAILRRLLRRLV
                     IHNRKSDQGAQWSVVRSFEFHPVWEPDPWS"
     CDS             complement(6922..6933)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     CDS             7426..9210
                     /codon_start=1
                     /transl_table=11
                     /product="phiBT1 integrase"
                     /label=phiBT1 integrase
                     /protein_id="QCO69797.1"
                     /translation="MSPFIAPDVPEHLLDTVRVFLYARQSKGRSDGSDVSTEAQLAAGR
                     ALVASRNAQGGARWVVAGEFVDVGRSGWDPNVTRADFERMMGEVRAGEGDVVVVNELSR
                     LTRKGAHDALEIDNELKKHGVRFMSVLEPFLDTSTPIGVAIFALIAALAKQDSDLKAER
                     LKGAKDEIAALGGVHSSSAPFGMRAVRKKVDNLVISVLEPDEDNPDHVELVERMAKMSF
                     EGVSDNAIATTFEKEKIPSPGMAERRATEKRLASIKARRLNGAEKPIMWRAQTVRWILN
                     HPAIGGFAFERVKHGKAHINVIRRDPGGKPLTPHTGILSGSKWLELQEKRSGKNLSDRK
                     PGAEVEPTLLSGWRFLGCRICGGSMGQSQGGRKRNGDLAEGNYMCANPKGHGGLSVKRS
                     ELDEFVASKVWARLRTADMEDEHDQAWIAAAAERFALQHDLAGVADERREQQAHLDNVR
                     RSIKDLQADRKAGLYVGREELETWRSTVLQYRSYEAECTTRLAELDEKMNGSTRVPSEW
                     FSGEDPTAEGGIWASWDVYERREFLSFFLDSVMVDRGRHPETKKYIPLKDRVTLKWAEL
                     LKEEDEASEATERELAAL"
ORIGIN
        1 ggatccttaa ttaaggcata tggacactcc ttacttagac tgtcgtattc tcctggccac
       61 gactttacaa caccgcacag catgttgtca aagcagagac ggttcgaatg tgaacagata
      121 tcgaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      181 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      241 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      301 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      961 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1201 tgagattatc aaaaaggatc ttcacctaga tccttttggt tcatgtgcag ctccatcagc
     1261 aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta
     1321 tgatcgactg atgtcatcag cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc
     1381 cgagctcggt acccggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
     1441 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
     1501 gaatcgaaat ctcgtgatgg caggttgggc gtcgcttact agtagctcca tcagcaaaag
     1561 gggatgataa gtttatcacc aatttaaatt gggcgtcgct tggtcggtca tttcgaaccc
     1621 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     1681 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     1741 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     1801 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     1861 ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt
     1921 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     1981 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     2041 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     2101 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     2161 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     2221 cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg
     2281 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     2341 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     2401 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat
     2461 cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
     2521 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct tgctgtccat
     2581 aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg ctttctcttt
     2641 gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg gggtcagcac
     2701 cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt gcgccctgag
     2761 tgcttgcggc agcgtcgcca ttgatgcggg ccagctcgcg gacgtgctca tagtccacga
     2821 cgcccgtgat tttgtagccc tggccgacgg ccagcaggta ggccgacagg ctcatgccgg
     2881 ccgccgccgc cttttcctca atcgctcttc gttcgtctgg aaggcagtac accttgatag
     2941 gtgggctgcc cttcctggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta
     3001 cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg
     3061 aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc
     3121 ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg
     3181 tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg
     3241 gttatgcagc ggaaaagatc cgtcgacctg cactcgagat ctcatgcgag tgtccgttcg
     3301 agtggcggct tgcgcccgat gctagtcgcg gttgatcggc gatcgcaggt gcacgcggtc
     3361 gatcttgacg gctggcgaga ggtgcgggga ggatctgacc gacgcggtcc acacgtggca
     3421 ccgcgatgct gttgtgggca caatcgtgcc ggttggtagg atccacatat gaaacgagac
     3481 ctaccaagca cgttccgcgg ctcccgcacg ccgggcgagc cgtggctcgg ctacatcagg
     3541 gtcagcacat ggcgcgagga gaagatcagc ccggagctac agcaatccgc gatcgagtca
     3601 tgggccgccc gaacgggacg tcggatcgtc gactggatcg ttgacctcga cgctacgggc
     3661 cgcaacttca agcggaagat catgggcggc atccagcgcg tcgagggccg cgaggccgtc
     3721 ggaatcgccg tatggaagtt cagccgcttc ggccggaacg acctgggcat cgctatcaac
     3781 ctcgcccgcc tcgaacaggc cggcggagac ctcgcgtccg ccacggaaga agtcgacgcc
     3841 cgcacggccg tcggacgctt caaccgcgcg attctgttcg acctcgccgt attcgagtcg
     3901 gatcgggccg gcgaacagtg gaaggagacg cacgcccacc gtcgggccct gaaactcccc
     3961 gccaccgggc gtcagcggtt cggctacgtg tggcacccga ggcgcgtacc cgacctcacc
     4021 gcgccgggcg ggttccgcct gcaagaggag cgctacgaac ggcacccaga gttcgccccc
     4081 gtggcggccg aactctacga gcgaaagctc gccggacagg ggttctccca gctcgcctac
     4141 tggctgaacg acgaactact catccccacc acacggggca accgctgggg caccaacacc
     4201 gttcagcgct acctcgactc cggattcgcc gccggcctgc tacgcgtcca cgacccggag
     4261 tgccggtgca agctgggaca ggaccacttc tctgcgtgca aagaaaaccg gtggctctgg
     4321 ctccccgggg cacagccggc gctcatcgtt cccgagcagt ggaaggagta cggggcgcac
     4381 agagagcaga cgcgcaagac gcccccgcgc gcccgccggg cctcataccc gaccagcggc
     4441 atcatgcggc acggacactg ccgcgggacg gccgttgcgc gatccggccg cgacgggaag
     4501 ggcggattcg ttccgggcca cgtgttcgtg tgcttcaacc gcaggaacaa ggggaagtcg
     4561 gcctgtgagc ccggcctcta cgtgcgccgc gacgaagtcg aggccgaggt gttgaagtgg
     4621 ctcgccgaca cggtcgccga cgacatcgac aacgcgccgg cccttcccgc tcagcgcacc
     4681 gcgccgggca ccgcgccgga cccgcgggcc cgcctggtcg aggagcgcac ccgcacggaa
     4741 gccgagttgg ccaagatcga gggtgcactc gaccgtctcg tcacggatta cgcgctggac
     4801 ccggacaagt acccggcgga cacgttcggg cgcgtacgcg accagctact cggtaagaag
     4861 ggcgacatca tcaagcactt gaagtcgctc agcgaggttg aagtcgcgcc cacgcgggag
     4921 gagttccgcc ccctgatcgt cgggctcttg caggagtggg acatcctgca caccaccgag
     4981 aagaacgcca tcctgcgccg cctgctgcgc cgtctggtca tccacaaccg taagagcgat
     5041 cagggcgcac agtggagtgt cgtgcgcagc ttcgagtttc atcccgtgtg ggagcccgac
     5101 ccctggtctt gaccgctgga gcgcaaacca tgcaggcgcg cttgattgtt tctccgcctg
     5161 ctgccaccct ctgaaaaccg cacctcagtg cagggagagg gggaacgatg ctcgcgagtc
     5221 ctttagagac actgacccac gtcagtggat ctaaaggacc acatcggagc gcgaagaacg
     5281 gccccggtac ctacctcagg taccggggcc gttccatgtg cggcccaccg cgtgtttaaa
     5341 cttgcaccgt cctcgctgga gtcgctgacg aggcgcgtgg tgggcacgtg gagggctgcg
     5401 gccagttgcc gggcggtctc cagacgggtg tcgctacgct ccccttgctc cagcttccgg
     5461 atcagcgaga gggacacccc ggagctcttg gcgagctctt gctgactcat gccccgacgc
     5521 ttgcgtacgt cgcggagctg cttaccaatg tcggcactca tgagtcaaga ctacggcgcg
     5581 taagctggtg agcacatgga aaagcccccg cttcctggcg ggggcgaggg tccgaggcat
     5641 gttccccaaa gcgataccac ttgaagcagt ggtactgctt gtgggtacac tctgcgggtg
     5701 atgaatcgag gggggcccac tgtacgggcc gacatctacg tccgaatcag cctggaccgc
     5761 acaggggaag agctcggggt cgagcgccag gaggagtcgt gtcgcgagct ctgcaagagc
     5821 ctcggcatgg aggtggggca ggtgtgggtc gacaacgacc tgagcgccac caagaagaac
     5881 gtcgtccgcc ctgacttcga ggcgatgatc gcgagcaacc cgcaggcgat cgtctgctgg
     5941 cacaccgacc ggctcatccg cgtcacgcgg gacctggagc gggtgatcga cctcggagtc
     6001 aacgtccacg ccgtgatggc cggacacctg gacctgtcca ccccggccgg ccgagccgtc
     6061 gcccgcacgg tgacggcctg ggccacgtac gagggcgagc agaaggctga gcgccagaag
     6121 ctcgccaaca tccagaacgc ccgcgccggc aagccgtaca cccccggcat ccgccccttc
     6181 gggtacggcg acgaccacat gaccatcgtg acggccgagg cggacgccat ccgcgacggc
     6241 gcgaagatga tcctcgacgg ctggtccctg tcggccgtgg ctcgctactg ggaggagctc
     6301 aagctccagt cgccccggag tatggccgca ggcggcaagg gctggtctct gcggggcgta
     6361 aagaaggtgc tgacctcccc gcgctacgtc gggcggtcca gctacctcgg ggaggtcgtg
     6421 ggcgatgctc agtggccgcc catcctcgac ccggacgtct actacggggt cgtggccatc
     6481 ctgaacaacc ccgaccgctt cagcgggggc cctcggaccg gccgcacccc cggcacgctg
     6541 ctcgcaggca tcgccttgtg cggtgagtgc ggcaagacgg tcagtggacg cggctaccga
     6601 ggtgtcctgg tctacggatg taaggacacg cacactcgga cgcctcggag catcgctgac
     6661 ggccgcgcga gcagctcgac cctcgcccgg ctcatgttcc ccgacttcct gcccggcctc
     6721 ctggcctctg ggcaggccga ggacggccag tcggcagcat ccaagcactc ggaggcccag
     6781 acgctgcgcg agcgccttga cgggctggct acggcctacg cggagggtgc gatcagcctg
     6841 tctcagatga cggccggctc ggaagcactg cggaagaagc tggaggtgat cgaagccgac
     6901 ctcgtgggct cggcaggcat cccgcccttc gatccagtgg ccggagtggc tggcctgatc
     6961 tccggctggc ccaccacgcc tctcccgacg cgtcgagcat gggtggactt ctgcctggtg
     7021 gtcacgctga acacccagaa ggggcgccat gcgtcgagca tgaccgtgga cgaccacgtc
     7081 accatcgagt ggcgagacgt ggccgagtag cagatacgac gaagccccgg ctaccccctt
     7141 ctgagggtgc cggggctttc gtgtgtcagt tgtggtggcc gctctccagg cggtcgatct
     7201 cttcgaggga cgcctcgtag ttgccggcct cttggaggag gagcaggcgg aggtcggcga
     7261 gcttgacctg cgcccacacg gggaatgcat tggcgccgga cggggcttca gacgtttcgg
     7321 gtgctgggtt gttgtctctg gacagtgatc catgggaaac tactcagcac caccaatgtt
     7381 cccaaaagaa agcgcaggtc agcgcccatg agccaagatc taggcatgtc gcccttcatc
     7441 gctcccgacg tccctgagca ccttctagac actgttcgcg tcttcctgta cgcgcgtcag
     7501 tctaagggcc ggtccgacgg ctcagacgtg tcgaccgaag cacagctagc ggccggtcgt
     7561 gcgttggtcg cgtctcgcaa cgcccagggg ggtgcgcgct gggtcgtggc aggtgagttc
     7621 gtggacgtcg ggcgctccgg ctgggacccg aacgtgaccc gtgccgactt cgagcgcatg
     7681 atgggcgaag tccgcgccgg cgaaggtgac gttgtcgttg tgaatgagct ttcccggctc
     7741 actcgcaagg gcgcccatga cgcgctcgaa atcgacaacg aattgaagaa gcacggcgtg
     7801 cgcttcatgt cggttcttga gccgttcctt gacacgtcta cccctatcgg cgtcgccatt
     7861 ttcgcgctga tcgctgccct tgcgaaacag gacagtgacc tgaaggcgga gcgcctgaag
     7921 ggtgcgaaag acgagattgc cgcgctgggt ggcgttcact cgtcttccgc cccgttcgga
     7981 atgcgcgccg tgcgcaagaa ggtcgataat ctcgtgatct ccgttcttga gccggacgaa
     8041 gacaacccgg atcacgtcga gctagttgag cgcatggcga aaatgtcgtt cgaaggcgtg
     8101 tccgacaacg ccattgcaac gaccttcgag aaggaaaaga tcccgtcgcc cggaatggct
     8161 gagagacgcg ccacggaaaa gcgtcttgcg tccatcaagg cacgtcgcct gaacggcgct
     8221 gaaaagccga tcatgtggcg cgctcaaacg gtccgatgga ttctcaacca tcccgcaatc
     8281 ggcggtttcg cattcgagcg tgtgaagcac ggtaaggcgc acatcaacgt catacggcgc
     8341 gaccccggcg gcaagccgct aacgccccac acgggcattc tcagcggctc gaagtggctt
     8401 gagcttcaag agaagcgttc cgggaagaat ctcagcgacc ggaagcctgg ggccgaagtc
     8461 gaaccgacgc ttctgagcgg gtggcgtttc ctggggtgcc gaatctgcgg cggctcaatg
     8521 ggtcagtccc agggtggccg taagcgcaac ggcgaccttg ccgaaggcaa ttacatgtgc
     8581 gccaacccga aggggcacgg cggcttgtcg gtcaagcgca gcgaactgga cgaattcgtt
     8641 gcttcgaagg tgtgggcacg gctccgcaca gccgacatgg aagatgaaca cgatcaggca
     8701 tggattgccg ccgctgcgga gcgcttcgcc cttcagcacg acctagcggg ggtggccgat
     8761 gagcggcgcg aacaacaggc gcacctagac aacgtgcggc gctccatcaa ggaccttcag
     8821 gcggaccgta aggccggtct gtacgtcggg cgtgaagagc tggaaacgtg gcgctcaacg
     8881 gtgctgcaat accggtccta cgaagcggag tgcacgaccc gactcgctga gcttgacgag
     8941 aagatgaacg gcagcacccg cgttccgtct gagtggttca gcggcgaaga cccgacggcc
     9001 gaagggggca tctgggcaag ctgggacgtg tacgagcgtc gggagttcct gagcttcttc
     9061 cttgactccg tcatggtcga ccgggggcgc caccctgaga cgaagaaata catccccctg
     9121 aaggaccgtg tgacgctcaa gtgggcggag ctgctgaagg aggaagacga agcgagcgaa
     9181 gccactgagc gggagcttgc ggcgctgtag cgcacagcgg gaggggtcga gccggcggac
     9241 ggttcggccc cttttttggc cttgaaatcg ttagttaggc tagcggccgc cgcaattgat
     9301 ttaaattgaa gccgccgaag ccccgaagct tccccttgac caatactagt agttccttcg
     9361 tcaccatagc gggcagggag c
//
