LOCUS       40924_28721        4460 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLS13, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Hays LB, Chen Y-S.A., Hu JC.
  TITLE     A two-hybrid system for characterization of protein-protein 
            interactions in E. coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Hays LB, Chen Y-S.A., Hu JC.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 4460)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4460)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-JUL-1999) Biochemistry "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      11..48
                     /label=operatorless mutant lacUV5 promoter
                     /note="operatorless mutant lacUV5 promoter"
                     /regulatory_class="promoter"
     CDS             73..657
                     /codon_start=1
                     /product="cI-GCN4IINI fusion protein"
                     /label=cI-GCN4IINI fusion protein
                     /note="cpntains N-terminal DNA binding domain from
                     bacteriophage lambda cI repressor, mutant GCN4 leucine 
                     zipper and linker for fusion to additional protein domains"
                     /protein_id="AAD50897.1"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTHMKQLEDKIEELLSKIYHLENENARLKKLI
                     GERGGQLNASGSGAANKARKEAELAAATAEQ"
     terminator      658..705
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(1063..1607)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        1719..1747
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             1795..2982
                     /label=TcR
                     /note="tetracycline efflux protein"
ORIGIN
        1 aattctaggc tttatgcttt atgcttccgg ctcgtacatt gtgtggaggt acctctagac
       61 aggaaacagc gtatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca
      121 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct
      181 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat
      241 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa
      301 tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg
      361 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca
      421 cctaagctta gaacctttac caaaggtgat gcggagagat gggtgtcgac acatatgaaa
      481 cagctggaag acaaaatcga agagctcctg tctaaaatct accacctcga gaacgaaaac
      541 gcgcgcctga aaaaactaat cggtgaacgt ggtggccaat tgaacgcgtc gggatccggc
      601 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      661 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      721 atatgcccgg atctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag
      781 caccgccgga catcagcgct agcggagtgt atactggctt actatgttgg cactgatgag
      841 ggtgtcagtg aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga
      901 atatgtgata caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc
      961 gactgcggcg agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga
     1021 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
     1081 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
     1141 ataaagatac caggcgtttc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt
     1201 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
     1261 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
     1321 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa
     1381 agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg
     1441 ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac
     1501 ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt
     1561 tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta
     1621 ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg
     1681 aagtcagccc catacgatat aagttgtaat tctcatgttt gacagcttat catcgattag
     1741 ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa
     1801 tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc
     1861 ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc
     1921 agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt
     1981 ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga
     2041 gccactatcg actacgcgat catggcgacc acacccgtcc tgtggattct ctacgccgga
     2101 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     2161 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     2221 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt acatgcacca
     2281 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     2341 gagtcgcata agggagagcg ccgaccgatg cccttgagag ccttcaaccc agtcagctcc
     2401 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     2461 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
     2521 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
     2581 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
     2641 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
     2701 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
     2761 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
     2821 cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg
     2881 gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc
     2941 cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc
     3001 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
     3061 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
     3121 gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc
     3181 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac
     3241 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa
     3301 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcccctacgt
     3361 gctgctgaag ttgcccgcaa cagagagtgg aaccaaccgg tgataccacg atactatgac
     3421 tgagagtcaa cgccatgagc ggcctcattt cttattctga gttacaacag tccgcaccgc
     3481 tgtccggtag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     3541 tcttctttat catgcaactc gtaggacagg tgccggcagc gcccaacagt cccccggcca
     3601 cggggcctgc caccataccc acgccgaaac aagcgccctg caccattatg ttccggatct
     3661 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct
     3721 aaccgttttt atcaggctct gggaggcaga ataaatgatc atatcgtcaa ttattacctc
     3781 cacggggaga gcctgagcaa actggcctca ggcatttgag aagcacacgg tcacactgct
     3841 tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg
     3901 ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta
     3961 tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct taaaaaaatt
     4021 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     4081 ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     4141 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     4201 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     4261 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     4321 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     4381 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     4441 cgtctttcat tgccatacgg
//
