LOCUS       V004939                 6743 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004939
VERSION     V004939
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Fast and simple in vivo photographic assessment of luciferase
            properties in E. coli cells to screen and compare mutants of beetle
            luciferases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Koksharov MI.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2016) Department of Chemical Enzymology, Faculty
            of Chemistry, Lomonosov Moscow State University, Leninskie gory,
            Bld. 1/11, Moscow 119991, Russia
REFERENCE   3  (bases 1 to 6743)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6743)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-FEB-2016) Department of Chemical Enzymology, Faculty of
            Chemistry, Lomonosov Moscow State University, Leninskie gory, Bld.
            1/11, Moscow 119991, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(8..757)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             976..1554
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     primer_bind     1584..1611
                     /label="F_start_pLRx"
                     /note="F_start_pLRx"
     CDS             1615..3246
                     /note="Luciferin 4-monooxygenase from Luciola mingrelica.
                     Accession#: Q26304"
                     /label="Luciferin 4-monooxygenase"
     CDS             3259..3276
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      3343..3390
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      3427..3882
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3909..4013
                     /label="AmpR promoter"
     CDS             4014..4871
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5045..5633
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5819..5959)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(6064..6252)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 gtacttaact tttaaagtat gggcaatcaa ttgctcctgt taaaattgct ttagaaatac
       61 tttggcagcg gtttgttgta ttgagtttca tttgcgcatt ggttaaatgg aaagtgaccg
      121 tacgcttact gcagcctaat atttttgaaa tatcccaaga gctctttcct tcgcatgccc
      181 acgctaaaca ttctttttct cttttggtta aatcgttgtt tgatttatta tttgctatat
      241 ttatttttcg ataattatca actagagaag gaacaattaa tggtatgttc atacatgcat
      301 gtaaaaataa actatctata tagttgtctt tctctgaatg tgcaaaacta agcattccga
      361 agccattatt agcagtatga atagggaaac taaacccagt gataagacct gatgatttcg
      421 cttctttaat tacatttgga gattttttat ttacagcatt gttttcaaat atattccaat
      481 taattggtga atgattggag ttagaataat ctactatagg atcatatttt attaaattag
      541 cgtcatcata atattgcctc cattttttag ggtaattatc tagaattgaa atatcagatt
      601 taaccataga atgaggataa atgatcgcga gtaaataata ttcacaatgt accattttag
      661 tcatatcaga taagcattga ttaatatcat tattgcttct acaagcttta attttattaa
      721 ttattctgta agtgtcgtcg gcatttatgt ctttcatacc catctcttta tccttaccta
      781 ttgtttgtcg caagttttgc gtgttatata tcattaaaac ggtaatagat tgacatttga
      841 ttctaataaa ttggattttt gtcacactat tatatcgctt gaaatacaat tgtttaacat
      901 aagtacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag tcgattaaac
      961 gcaagggagg ttggtatgac tataatgata aaaaaatcgg attttttggc aattccatcg
     1021 gaggagtata aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg
     1081 gacttagttg tagaaaataa ccttgaatca gatgagtatg ataactcaaa tgcagaatat
     1141 atttatgctt gtgatgatac tgaaaatgta agtggatgct ggcgtttatt acctacaaca
     1201 ggtgattata tgctgaaaag tgtttttcct gaattgcttg gtcaacagag tgctcccaaa
     1261 gatcctaata tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc caagataaat
     1321 aactctgcta gtgaaattac aatgaaacta tttgaagcta tatataaaca cgctgttagt
     1381 caaggtatta cagaatatgt aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt
     1441 attaaagttc cttgtcatcg tattggagac aaagaaattc atgtattagg tgatactaaa
     1501 tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt aaattaatat
     1561 tgttaaatca ttaatttatt ttaaatacta agtatattat aggaggctag caccatggaa
     1621 aaggaggaga atgttgtata tggccctctg ccattctacc ccattgaaga aggatcagct
     1681 ggaattcagt tgcataagta catgcatcaa tatgccaaac ttggagcaat tgctttttcg
     1741 aacgccctta ctggagttga catttcttac caagaatact ttgatattac atgtcgttta
     1801 gctgaggcca tgaaaaactt tggtatgaaa ccggaagaac atattgcttt gtgcagtgaa
     1861 aattgtgaag aatttttcat ccctgtactt gctggtcttt acattggggt agctgttgca
     1921 cctactaatg aaatttacac attgcgtgaa cttaatcata gtttgggcat cgcacaacca
     1981 actattgtat tcagctcgag aaaaggctta cctaaagttt tagaagtgca aaaaacagtt
     2041 acatgcatca aaaaaattgt tattttagat agtaaagtaa actttggggg ccacgattgt
     2101 atggaaactt ttattaagaa acatgtagaa ttaggttttc aaccaagtag ctttgtaccc
     2161 attgatgtaa agaaccgtaa acaacacgtt gctttgctta tgaattcttc tggctctact
     2221 ggtttaccta aaggtgtacg aattacccac gaaggtgcag ttacaagatt ctcacacgct
     2281 aaggatccaa tttacggaaa ccaagtttca cctggtactg ctattttaac tgtcgttccg
     2341 ttccatcatg gatttggaat gtttaccact ttaggatact ttgcttgtgg ataccgtgtt
     2401 gtaatgttaa caaaatttga tgaagaacta tttttgagaa ctttgcaaga ttataagtgt
     2461 accagtgtta ttctggtacc aacgttattt gctattctca acaagagtga attgatcgat
     2521 aagttcgatt tatctaatct aactgaaatt gcttctggtg gagctccttt ggcaaaagaa
     2581 gttggcgaag cagtcgctag aagatttaat ctacccggtg tccgtcaggg ttacggatta
     2641 acagaaacga catctgcatt tattattacc ccagaaggtg atgataaacc tggagcatct
     2701 ggaaaagtag tacccttatt caaagtaaaa gttattgatc ttgacactaa aaaaactttg
     2761 ggtgtcaacc gacgaggaga gatctgtgta aaaggaccca gtcttatgtt aggctactcg
     2821 aacaatccgg aagcaacaag agaaactatt gatgaagagg gttggttgca cacaggagat
     2881 attggatatt acgacgaaga cgaacatttc ttcattgtcg accgtttgaa atcattaatc
     2941 aaatacaagg ggtaccaggt accacctgct gaattggaat ccgttctttt gcaacatcca
     3001 aatatatttg atgctggtgt ggctggtgtc cccgatcccg atgctggcga acttccaggg
     3061 gctgtagttg taatggaaaa aggaaaaact atgactgaaa aggaaattgt ggattatgtt
     3121 aatagtcaag tagtgaacca caaacgtctg cgtggtggcg ttcgttttgt ggatgaagta
     3181 cctaaaggtc taactggtaa aattgatgct aaagtaatta gagaaattct taagaaacca
     3241 caatcgggcc cagtcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
     3301 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     3361 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
     3421 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3481 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3541 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3601 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3661 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3721 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     3781 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     3841 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcaggtggc acttttcggg
     3901 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3961 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4021 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4081 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4141 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4201 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4261 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4321 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4381 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4441 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4501 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     4561 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4621 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4681 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4741 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4801 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4861 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4921 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5161 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     5221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5701 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     5761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     5821 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     5881 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     5941 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     6001 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     6061 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc
     6121 gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt
     6181 ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc
     6241 ctgtttggtc acttgatgcc tccgtgtaag ggggaatttc tgttcatggg ggtaatgata
     6301 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
     6361 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
     6421 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag
     6481 cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
     6541 agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt
     6601 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
     6661 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt
     6721 ggccaggacc caacgctgcc cga
//
