LOCUS       V004944                 6746 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004944
VERSION     V004944
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Fast and simple in vivo photographic assessment of luciferase
            properties in bacterial cells to screen and compare mutants of
            beetle luciferases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Koksharov MI.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2016) Department of Chemical Enzymology, Faculty
            of Chemistry, Lomonosov Moscow State University, Leninskie gory,
            Bld. 1/11, Moscow, Moscow 119991, Russia
REFERENCE   3  (bases 1 to 6746)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6746)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-FEB-2016) Department of Chemical Enzymology, Faculty of
            Chemistry, Lomonosov Moscow State University, Leninskie gory, Bld.
            1/11, Moscow, Moscow 119991, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6746
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(8..757)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             976..1554
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     primer_bind     1584..1611
                     /label="F_start_pLRx forward primer binding region"
                     /note="F_start_pLRx forward primer binding region"
     CDS             3262..3279
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      3346..3393
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      3430..3885
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3912..4016
                     /label="AmpR promoter"
     CDS             4017..4874
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5048..5636
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5822..5962)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(6067..6255)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 gtacttaact tttaaagtat gggcaatcaa ttgctcctgt taaaattgct ttagaaatac
       61 tttggcagcg gtttgttgta ttgagtttca tttgcgcatt ggttaaatgg aaagtgaccg
      121 tacgcttact gcagcctaat atttttgaaa tatcccaaga gctctttcct tcgcatgccc
      181 acgctaaaca ttctttttct cttttggtta aatcgttgtt tgatttatta tttgctatat
      241 ttatttttcg ataattatca actagagaag gaacaattaa tggtatgttc atacatgcat
      301 gtaaaaataa actatctata tagttgtctt tctctgaatg tgcaaaacta agcattccga
      361 agccattatt agcagtatga atagggaaac taaacccagt gataagacct gatgatttcg
      421 cttctttaat tacatttgga gattttttat ttacagcatt gttttcaaat atattccaat
      481 taattggtga atgattggag ttagaataat ctactatagg atcatatttt attaaattag
      541 cgtcatcata atattgcctc cattttttag ggtaattatc tagaattgaa atatcagatt
      601 taaccataga atgaggataa atgatcgcga gtaaataata ttcacaatgt accattttag
      661 tcatatcaga taagcattga ttaatatcat tattgcttct acaagcttta attttattaa
      721 ttattctgta agtgtcgtcg gcatttatgt ctttcatacc catctcttta tccttaccta
      781 ttgtttgtcg caagttttgc gtgttatata tcattaaaac ggtaatagat tgacatttga
      841 ttctaataaa ttggattttt gtcacactat tatatcgctt gaaatacaat tgtttaacat
      901 aagtacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag tcgattaaac
      961 gcaagggagg ttggtatgac tataatgata aaaaaatcgg attttttggc aattccatcg
     1021 gaggagtata aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg
     1081 gacttagttg tagaaaataa ccttgaatca gatgagtatg ataactcaaa tgcagaatat
     1141 atttatgctt gtgatgatac tgaaaatgta agtggatgct ggcgtttatt acctacaaca
     1201 ggtgattata tgctgaaaag tgtttttcct gaattgcttg gtcaacagag tgctcccaaa
     1261 gatcctaata tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc caagataaat
     1321 aactctgcta gtgaaattac aatgaaacta tttgaagcta tatataaaca cgctgttagt
     1381 caaggtatta cagaatatgt aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt
     1441 attaaagttc cttgtcatcg tattggagac aaagaaattc atgtattagg tgatactaaa
     1501 tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt aaattaatat
     1561 tgttaaatca ttaatttatt ttaaatacta agtatattat aggaggctag caccatggaa
     1621 gatgcaaaaa atattatgca cggtccagcg ccattctatc ctttggagga tggaactgct
     1681 ggagaacaat tgcacaaagc aatgaagagg tatgcacagg ttccagggac aatagctttt
     1741 actgatgcgc atgcagaggt aaatattaca tattccgaat attttgaaat ggcttgccgg
     1801 ttagccgaaa ctatgaagag gtacggactt ggtttacaac accacattgc tgtttgtagc
     1861 gaaaattctc ttcagttttt tatgcctgta tgcggtgctc tatttattgg agttggagtt
     1921 gcaccaacaa atgatattta caatgaacgt gaattataca acagtttgtc catatcacaa
     1981 cctacaatag tattctgttc caaaagagcg ctgcaaaaaa tcctaggggt acaaaagaaa
     2041 ttacctataa ttcagaaaat tgttattctg gattctcgag aggattatat ggggaaacaa
     2101 tctatgtact cgttcattga atctcattta cctgcaggtt ttaatgaata tgattacata
     2161 ccggattcat ttgaccgcga aacagcaaca gcacttataa tgaattcatc gggatctact
     2221 ggattaccca agggagttga gcttactcac caaaatgttt gtgttagatt ttctcactgc
     2281 agagatcctg tgtttggtaa tcaaattatt cccgatactg cgattttaac agttatacca
     2341 tttcatcatg gttttggaat gtttacaaca ctaggatatt taacgtgtgg atttcgtatt
     2401 gtgcttatgt atagatttga agaggaatta tttttacgat cacttcaaga ttataaaatt
     2461 caaagtgcgt tgctggtacc taccctattt tcattctttg ccaaaagcac cttagtcgat
     2521 aaatacgatt tatccaactt acatgaaatt gcgtctggtg gagctcccct cgcgaaagaa
     2581 gttggagaag ctgtagcaaa acgttttaag ctgccgggaa tacgacaagg gtatggactt
     2641 actgaaacta cctcagctat tataattaca ccagaagggg atgataaacc aggagcatgt
     2701 ggtaaagttg ttccattctt ttctgccaaa attgttgatc tggatacggg taaaactttg
     2761 ggtgttaatc agagggggga attatgtgtg aaaggcccaa tgataatgaa gggttacgta
     2821 aacaacccag aagcaacaag tgcattgata gacaaagatg gatggttaca ctctggtgac
     2881 atagcttact acgacaaaga tggtcacttc ttcatagtag atcgtttgaa atcgttaatt
     2941 aaatacaaag gttatcaggt accgcctgcc gaattagaat cgatattgct gcaacatccc
     3001 ttcatatttg atgcaggtgt tgcaggaatt cccgacccag atgccggtga acttcctgca
     3061 gccgttgttg tcttagagga aggcaaaacg atgactgaac aagaagtgat ggattatgtt
     3121 gcgggacaag tcactgcttc taagcgttta cgtggaggag ttaagtttgt ggacgaagta
     3181 cctaaaggtc taactggaaa gattgatgga agaaaaatca gggagatcct tatgatggga
     3241 aaaaaatcgg gcccagtcga gcaccaccac caccaccact gagatccggc tgctaacaaa
     3301 gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt
     3361 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg
     3421 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3481 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3541 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3601 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     3661 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3721 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     3781 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3841 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcaggt ggcacttttc
     3901 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3961 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4021 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4081 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4141 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4201 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4261 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4321 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4381 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4441 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4501 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4561 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     4621 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     4681 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     4741 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     4801 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     4861 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     4921 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4981 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5041 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5101 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5161 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     5221 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5281 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5341 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5401 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5461 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5521 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5581 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5641 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5701 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5761 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5821 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca
     5881 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     5941 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     6001 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     6061 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     6121 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
     6181 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     6241 ttcctgtttg gtcacttgat gcctccgtgt aagggggaat ttctgttcat gggggtaatg
     6301 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
     6361 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa
     6421 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc
     6481 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt
     6541 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac
     6601 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca
     6661 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc
     6721 cgtggccagg acccaacgct gcccga
//
