LOCUS       V004945                 6731 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004945
VERSION     V004945
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Fast and simple in vivo photographic assessment of luciferase
            properties in bacterial cells to screen and compare mutants of
            beetle luciferases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6731)
  AUTHORS   Koksharov MI.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2016) Department of Chemical Enzymology, Faculty
            of Chemistry, Lomonosov Moscow State University, Leninskie gory,
            Bld. 1/11, Moscow, Moscow 119991, Russia
REFERENCE   3  (bases 1 to 6731)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6731)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-FEB-2016) Department of Chemical Enzymology, Faculty of
            Chemistry, Lomonosov Moscow State University, Leninskie gory, Bld.
            1/11, Moscow, Moscow 119991, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(8..757)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             976..1554
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     primer_bind     1584..1611
                     /label="F_start_pLRx forward primer binding region"
                     /note="F_start_pLRx forward primer binding region"
     RBS             3087..3095
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             3247..3264
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      3331..3378
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      3415..3870
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3897..4001
                     /label="AmpR promoter"
     CDS             4002..4859
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5033..5621
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5807..5947)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(6052..6240)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 gtacttaact tttaaagtat gggcaatcaa ttgctcctgt taaaattgct ttagaaatac
       61 tttggcagcg gtttgttgta ttgagtttca tttgcgcatt ggttaaatgg aaagtgaccg
      121 tacgcttact gcagcctaat atttttgaaa tatcccaaga gctctttcct tcgcatgccc
      181 acgctaaaca ttctttttct cttttggtta aatcgttgtt tgatttatta tttgctatat
      241 ttatttttcg ataattatca actagagaag gaacaattaa tggtatgttc atacatgcat
      301 gtaaaaataa actatctata tagttgtctt tctctgaatg tgcaaaacta agcattccga
      361 agccattatt agcagtatga atagggaaac taaacccagt gataagacct gatgatttcg
      421 cttctttaat tacatttgga gattttttat ttacagcatt gttttcaaat atattccaat
      481 taattggtga atgattggag ttagaataat ctactatagg atcatatttt attaaattag
      541 cgtcatcata atattgcctc cattttttag ggtaattatc tagaattgaa atatcagatt
      601 taaccataga atgaggataa atgatcgcga gtaaataata ttcacaatgt accattttag
      661 tcatatcaga taagcattga ttaatatcat tattgcttct acaagcttta attttattaa
      721 ttattctgta agtgtcgtcg gcatttatgt ctttcatacc catctcttta tccttaccta
      781 ttgtttgtcg caagttttgc gtgttatata tcattaaaac ggtaatagat tgacatttga
      841 ttctaataaa ttggattttt gtcacactat tatatcgctt gaaatacaat tgtttaacat
      901 aagtacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag tcgattaaac
      961 gcaagggagg ttggtatgac tataatgata aaaaaatcgg attttttggc aattccatcg
     1021 gaggagtata aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg
     1081 gacttagttg tagaaaataa ccttgaatca gatgagtatg ataactcaaa tgcagaatat
     1141 atttatgctt gtgatgatac tgaaaatgta agtggatgct ggcgtttatt acctacaaca
     1201 ggtgattata tgctgaaaag tgtttttcct gaattgcttg gtcaacagag tgctcccaaa
     1261 gatcctaata tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc caagataaat
     1321 aactctgcta gtgaaattac aatgaaacta tttgaagcta tatataaaca cgctgttagt
     1381 caaggtatta cagaatatgt aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt
     1441 attaaagttc cttgtcatcg tattggagac aaagaaattc atgtattagg tgatactaaa
     1501 tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt aaattaatat
     1561 tgttaaatca ttaatttatt ttaaatacta agtatattat aggaggctag caccatggag
     1621 agagagaaga acgtggtgta cggccccgag cccaagcacc ctctgggcaa cttcaccgcc
     1681 ggcgagatgc tgtacaacgc tctgcacaag cactcccaca tcccccaggc catcctggac
     1741 gtgatgggca acgagtccct ttcctaccag gagttcttcg acactactgt gaagctgggc
     1801 cagagcctcc agaactgtgg ctacaagatg aacgatgtcg tgtcgatctg tgcagagaac
     1861 aacaagagat tcttcatccc catcatctcc gcctggtaca tcggcatggt ggtggcccct
     1921 gtgaacgagg actatatccc agacgagctg tgtaaagtga ccggcatctc caagccgatc
     1981 ctggtcttca ccactaggaa gatcctgcct aaggttttgg aggttaaaga cagaaccaac
     2041 tacataaaga ggatcatcat actggactct gaagagaacc tgctgggctg cgagagcctg
     2101 cacaacttca tgtccaggta ctccgacaac aacctccaaa cattcaagcc tctgcactac
     2161 gaccctgtgg accaggtagc cgccatcctg tgctcctccg gcacaaccgg cctgcctaaa
     2221 ggcgtgatgc agacccacag gaacatctgt gtgagactca cacacgcatc tgaccccaga
     2281 gtgggtacac aactcatccc cggcgtatcc gtgctggcct acctgccatt cttccacgcc
     2341 ttcggcttca gtatcaacct gggctatttc atggtgggcc tgagagtggt gatgctccga
     2401 aggtttaacc aggaggtgtt cctgaaggcc atccaggact acgaggtgag gagcgtgatc
     2461 aacgttccct ccacaatcct gttcctgtcc aagagccctc tggtggacaa gtacgaccta
     2521 tccaccctgg cggagctgtg ctgtggagcc gctcctctgg cgaaggaggt ggccgagatc
     2581 gccgtgaaga ggctgaacct gccagggata cggtgtggct acggtctaac agagtctacc
     2641 tccgccaaca tccatactct gcacaacgag ttcaagtccg gctccctggg caaggtgaca
     2701 ccttacatgg ccgccaagat catcgacagg aacaccggcg aggccctggg tccaaaccag
     2761 gtgggcgagc tgtgcatctg gggacctatg gtaacaaaag gctatgtgaa caacccacag
     2821 gctactaagg aggccatcga cgacgacggc tggctgcact ctggcgactt cggctactac
     2881 gacgaggacg agtatttcta catcgtggac cggtacaagg agctgatcaa atacaagggc
     2941 tatcaggtcg cccctgtgga gctggaggag atcctccttc agcacccagg catcagggac
     3001 gtggccgtcg tgggtatccc tgacatcgag gccggcgagc tgccagccgg cttcgtggtg
     3061 aagcagcccg gcgcccaact caccgctaag gaggtgtacg acttcctggc ccagagggtg
     3121 tctcactcca agtacctgag gggcggcgta aggttcgtgg actctatccc caggaacgtg
     3181 acaggcaaga ttagtcgaaa agagctgagg gaggccctga tggagaaggc ttctggacca
     3241 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
     3301 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     3361 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
     3421 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3481 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3541 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3601 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     3661 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3721 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3781 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3841 attttaacaa aatattaacg tttacaattt caggtggcac ttttcgggga aatgtgcgcg
     3901 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3961 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4021 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     4081 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     4141 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     4201 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     4261 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4321 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4381 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4441 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4501 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
     4561 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4621 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4681 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4741 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4801 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4861 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4921 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4981 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5041 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5101 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5161 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5221 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5281 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5341 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5401 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5461 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5521 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5581 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5641 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5701 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5761 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     5821 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     5881 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     5941 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     6001 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     6061 accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag
     6121 cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt
     6181 taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac
     6241 ttgatgcctc cgtgtaaggg ggaatttctg ttcatggggg taatgatacc gatgaaacga
     6301 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     6361 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     6421 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     6481 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     6541 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     6601 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     6661 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca
     6721 acgctgcccg a
//
