LOCUS       V004947                 6745 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004947
VERSION     V004947
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Fast and simple in vivo photographic assessment of luciferase
            properties in E. coli cells to screen and compare mutants of beetle
            luciferases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Koksharov MI.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2016) Department of Chemical Enzymology, Faculty
            of Chemistry, Lomonosov Moscow State University, Leninskie gory,
            Bld. 1/11, Moscow 119991, Russia
REFERENCE   3  (bases 1 to 6745)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6745)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-FEB-2016) Department of Chemical Enzymology, Faculty of
            Chemistry, Lomonosov Moscow State University, Leninskie gory, Bld.
            1/11, Moscow 119991, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6745
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(8..757)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             976..1554
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     primer_bind     1584..1611
                     /label="F_start_pLRx"
                     /note="F_start_pLRx"
     CDS             1615..3249
                     /label="luciferase"
                     /note="firefly luciferase"
     CDS             3259..3276
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      3343..3390
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      3427..3882
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3909..4013
                     /label="AmpR promoter"
     CDS             4014..4871
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5045..5633
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5819..5961)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(6066..6254)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 gtacttaact tttaaagtat gggcaatcaa ttgctcctgt taaaattgct ttagaaatac
       61 tttggcagcg gtttgttgta ttgagtttca tttgcgcatt ggttaaatgg aaagtgaccg
      121 tacgcttact gcagcctaat atttttgaaa tatcccaaga gctctttcct tcgcatgccc
      181 acgctaaaca ttctttttct cttttggtta aatcgttgtt tgatttatta tttgctatat
      241 ttatttttcg ataattatca actagagaag gaacaattaa tggtatgttc atacatgcat
      301 gtaaaaataa actatctata tagttgtctt tctctgaatg tgcaaaacta agcattccga
      361 agccattatt agcagtatga atagggaaac taaacccagt gataagacct gatgatttcg
      421 cttctttaat tacatttgga gattttttat ttacagcatt gttttcaaat atattccaat
      481 taattggtga atgattggag ttagaataat ctactatagg atcatatttt attaaattag
      541 cgtcatcata atattgcctc cattttttag ggtaattatc tagaattgaa atatcagatt
      601 taaccataga atgaggataa atgatcgcga gtaaataata ttcacaatgt accattttag
      661 tcatatcaga taagcattga ttaatatcat tattgcttct acaagcttta attttattaa
      721 ttattctgta agtgtcgtcg gcatttatgt ctttcatacc catctcttta tccttaccta
      781 ttgtttgtcg caagttttgc gtgttatata tcattaaaac ggtaatagat tgacatttga
      841 ttctaataaa ttggattttt gtcacactat tatatcgctt gaaatacaat tgtttaacat
      901 aagtacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag tcgattaaac
      961 gcaagggagg ttggtatgac tataatgata aaaaaatcgg attttttggc aattccatcg
     1021 gaggagtata aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg
     1081 gacttagttg tagaaaataa ccttgaatca gatgagtatg ataactcaaa tgcagaatat
     1141 atttatgctt gtgatgatac tgaaaatgta agtggatgct ggcgtttatt acctacaaca
     1201 ggtgattata tgctgaaaag tgtttttcct gaattgcttg gtcaacagag tgctcccaaa
     1261 gatcctaata tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc caagataaat
     1321 aactctgcta gtgaaattac aatgaaacta tttgaagcta tatataaaca cgctgttagt
     1381 caaggtatta cagaatatgt aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt
     1441 attaaagttc cttgtcatcg tattggagac aaagaaattc atgtattagg tgatactaaa
     1501 tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt aaattaatat
     1561 tgttaaatca ttaatttatt ttaaatacta agtatattat aggaggctag ccatatggaa
     1621 gatgccaaaa acattaagaa gggcccagcg ccattctacc cactcgaaga cgggaccgcc
     1681 ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg tgcccggcac catcgccttt
     1741 accgacgcac atatcgaggt ggacattacc tacgccgagt acttcgagat gagcgttcgg
     1801 ctggcagaag ctatgaagcg ctatgggctg aatacaaacc atcggatcgt ggtgtgcagc
     1861 gagaatagct tgcagttctt catgcccgtg ttgggtgccc tgttcatcgg tgtggctgtg
     1921 gccccagcta acgacatcta caacgagcgc gagctgctga acagcatggg catcagccag
     1981 cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga tcctcaacgt gcaaaagaag
     2041 ctaccgatca tacaaaagat catcatcatg gatagcaaga ccgactacca gggcttccaa
     2101 agcatgtaca ccttcgtgac ttcccatttg ccacccggct tcaacgagta cgacttcgtg
     2161 cccgagagct tcgaccggga caaaaccatc gccctgatca tgaacagtag tggcagtacc
     2221 ggattgccca agggcgtagc cctaccgcac cgcaccgctt gtgtccgatt cagtcatgcc
     2281 cgcgacccca tcttcggcaa ccagatcatc cccgacaccg ctatcctcag cgtggtgcca
     2341 tttcaccacg gcttcggcat gttcaccacg ctgggctact tgatctgcgg ctttcgggtc
     2401 gtgctcatgt accgcttcga ggaggagcta ttcttgcgca gcttgcaaga ctataagatt
     2461 caatctgccc tgctggtgcc cacactattt agcttcttcg ctaagagcac tctcatcgac
     2521 aagtacgacc taagcaactt gcacgagatc gccagcggcg gggcgccgct cagcaaggag
     2581 gtaggtgagg ccgtggccaa acgcttccac ctaccaggca tccgccaggg ctacggcctg
     2641 acagaaacaa ccagcgccat tctgatcacc cccgaagggg acgacaagcc tggcgcagta
     2701 ggcaaggtgg tgcccttctt cgaggctaag gtggtggact tggacaccgg taagacactg
     2761 ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca tgatcatgag cggctacgtt
     2821 aacaaccccg aggctacaaa cgctctcatc gacaaggacg gctggctgca cagcggcgac
     2881 atcgcctact gggacgagga cgagcacttc ttcatcgtgg accggctgaa gagcctgatc
     2941 aaatacaagg gctaccaggt agccccagcc gaactggaga gcatcctgct gcaacacccc
     3001 aacatcttcg acgccggggt cgccggcctg cccgacgacg atgccggcga gctgcccgcc
     3061 gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga aggagatcgt ggactatgtg
     3121 gccagccagg ttacaaccgc caagaagctg cgcggtggtg ttgtgttcgt ggacgaggtg
     3181 cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc gcgagattct cattaaggcc
     3241 aagaagggac cactcgagca ccaccaccac caccactgag atccggctgc taacaaagcc
     3301 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     3361 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
     3421 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3481 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3541 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3601 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3661 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     3721 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     3781 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     3841 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcaggtggc acttttcggg
     3901 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     3961 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4021 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4081 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4141 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4201 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4261 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4321 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4381 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4441 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4501 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
     4561 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4621 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4681 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4741 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4801 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     4861 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     4921 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     4981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5161 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     5221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5701 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     5761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     5821 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac
     5881 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     5941 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     6001 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     6061 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     6121 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     6181 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     6241 tcctgtttgg tcacttgatg cctccgtgta agggggaatt tctgttcatg ggggtaatga
     6301 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
     6361 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
     6421 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
     6481 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
     6541 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
     6601 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
     6661 taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc
     6721 gtggccagga cccaacgctg cccga
//
