LOCUS       V004949                 6749 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004949
VERSION     V004949
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6749)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Thermostabilization of luciferase by in vivo directed evolution
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6749)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2010) Department of Chemistry, Lomonosov Moscow
            State University, Leninskie Gory, Bld. 1/11, GSP-3, Moscow 119991,
            Russia
REFERENCE   3  (bases 1 to 6749)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6749)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JUL-2010) Department of Chemistry, Lomonosov Moscow State
            University, Leninskie Gory, Bld. 1/11, GSP-3, Moscow 119991, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(8..757)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             976..1554
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     CDS             1615..3252
                     /note="Luciferin 4-monooxygenase from Luciola mingrelica.
                     Accession#: Q26304"
                     /label="Luciferin 4-monooxygenase"
     CDS             3265..3282
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      3349..3396
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      3433..3888
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3915..4019
                     /label="AmpR promoter"
     CDS             4020..4877
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5051..5639
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5825..5965)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(6070..6258)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 gtacttaact tttaaagtat gggcaatcaa ttgctcctgt taaaattgct ttagaaatac
       61 tttggcagcg gtttgttgta ttgagtttca tttgcgcatt ggttaaatgg aaagtgaccg
      121 tacgcttact gcagcctaat atttttgaaa tatcccaaga gctctttcct tcgcatgccc
      181 acgctaaaca ttctttttct cttttggtta aatcgttgtt tgatttatta tttgctatat
      241 ttatttttcg ataattatca actagagaag gaacaattaa tggtatgttc atacatgcat
      301 gtaaaaataa actatctata tagttgtctt tctctgaatg tgcaaaacta agcattccga
      361 agccattatt agcagtatga atagggaaac taaacccagt gataagacct gatgatttcg
      421 cttctttaat tacatttgga gattttttat ttacagcatt gttttcaaat atattccaat
      481 taattggtga atgattggag ttagaataat ctactatagg atcatatttt attaaattag
      541 cgtcatcata atattgcctc cattttttag ggtaattatc tagaattgaa atatcagatt
      601 taaccatgga atgaggataa atgatcgcga gtaaataata ttcacaatgt accattttag
      661 tcatatcaga taagcattga ttaatatcat tattgcttct acaagcttta attttattaa
      721 ttattctgta agtgtcgtcg gcatttatgt ctttcatacc catctcttta tccttaccta
      781 ttgtttgtcg caagttttgc gtgttatata tcattaaaac ggtaatagat tgacatttga
      841 ttctaataaa ttggattttt gtcacactat tatatcgctt gaaatacaat tgtttaacat
      901 aagtacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag tcgattaaac
      961 gcaagggagg ttggtatgac tataatgata aaaaaatcgg attttttggc aattccatcg
     1021 gaggagtata aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg
     1081 gacttagttg tagaaaataa ccttgaatca gatgagtatg ataactcaaa tgcagaatat
     1141 atttatgctt gtgatgatac tgaaaatgta agtggatgct ggcgtttatt acctacaaca
     1201 ggtgattata tgctgaaaag tgtttttcct gaattgcttg gtcaacagag tgctcccaaa
     1261 gatcctaata tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc caagataaat
     1321 aactctgcta gtgaaattac aatgaaacta tttgaagcta tatataaaca cgctgttagt
     1381 caaggtatta cagaatatgt aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt
     1441 attaaagttc cttgtcatcg tattggagac aaagaaattc atgtattagg tgatactaaa
     1501 tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt aaattaatat
     1561 tgttaaatca ttaatttatt ttaaatacta agtatattat aggaggctag caaaatggaa
     1621 atggaaaagg aggagaatgt tgtatatggc cctctgccat tctaccccat tgaagaagga
     1681 tcagctggaa ttcagttgca taagtacatg catcaatatg ccaaacttgg agcaattgct
     1741 ttttcgaacg cccttactgg agtcgacatt tcttaccaag aatactttga tattacatgt
     1801 cgtttagctg aggccatgaa aaactttggt atgaaaccgg aagaacatat tgctttgtgc
     1861 agtgaaaatt gtgaagaatt tttcatccct gtacttgctg gtctttacat tggggtagct
     1921 gttgcaccta ctaatgaaat ttacacattg cgtgaactta atcatagttt gggcatcgca
     1981 caaccaacta ttgtattcag ctcgagaaaa ggcttaccta aagttttaga agtgcaaaaa
     2041 acagttacat gcatcaaaaa aattgttatt ttagatagta aagtaaactt tgggggccac
     2101 gattgtatgg aaacttttat taagaaacat gtagaattag gttttcaacc aagtagcttt
     2161 gtacccattg atgtaaagaa ccgtaaacaa cacgttgctt tgcttatgaa ttcttctggc
     2221 tctactggtt tacctaaagg tgtacgaatt acccacgaag gtgcagttac aagattctca
     2281 cacgctaagg atccaattta cggaaaccaa gtttcacctg gtactgctat tttaactgtc
     2341 gttccgttcc atcatggatt tggaatgttt accactttag gatactttgc ttgtggatac
     2401 cgtgttgtaa tgttaacaaa atttgatgaa gaactatttt tgagaacttt gcaagattat
     2461 aagtgtacca gtgttattct ggtaccaacg ttatttgcta ttctcaacaa gagtgaattg
     2521 atcgataagt tcgatttatc taatctaact gaaattgctt ctggtggagc tcctttggca
     2581 aaagaagttg gcgaagcagt cgctagaaga tttaatctac ccggtgtccg tcagggttac
     2641 ggattaacag aaacgacatc tgcatttatt attaccccag aaggtgatga taaacctgga
     2701 gcatctggaa aagtagtacc cttattcaaa gtaaaagtta ttgatcttga cactaaaaaa
     2761 actttgggtg tcaaccgacg aggagagatc tgtgtaaaag gacccagtct tatgttaggc
     2821 tactcgaaca atccggaagc aacaagagaa actattgatg aagagggttg gttgcacaca
     2881 ggagatattg gatattacga cgaagacgaa catttcttca ttgtcgatcg tttgaaatca
     2941 ttaatcaaat acaaggggta ccaggtacca cctgctgaat tggaatccgt tcttttgcaa
     3001 catccaaata tatttgatgc tggtgtggct ggtgtccccg atcccgatgc tggcgaactt
     3061 ccaggggctg tagttgtaat ggaaaaagga aaaactatga ctgaaaagga aattgtggat
     3121 tatgttaata gtcaagtagt gaaccacaaa cgtctgcgtg gtggcgttcg ttttgtggat
     3181 gaagtaccta aaggtctaac tggtaaaatt gatgctaaag taattagaga aattcttaag
     3241 aaaccacaat cgggcccagt cgagcaccac caccaccacc actgagatcc ggctgctaac
     3301 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
     3361 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
     3421 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3481 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3541 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3601 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     3661 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     3721 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     3781 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     3841 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttca ggtggcactt
     3901 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     3961 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4021 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4081 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4141 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4201 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4261 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4321 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4381 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4441 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4501 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4561 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4621 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     4681 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     4741 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     4801 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     4861 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     4921 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4981 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5041 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5101 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5161 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     5221 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5281 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5341 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5401 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5461 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5521 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5581 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5641 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     5701 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5761 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5821 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     5881 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     5941 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     6001 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     6061 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
     6121 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
     6181 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
     6241 tttttcctgt ttggtcactt gatgcctccg tgtaaggggg aatttctgtt catgggggta
     6301 atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc
     6361 cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga
     6421 aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt
     6481 agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc
     6541 gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca
     6601 gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa
     6661 ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc
     6721 acccgtggcc aggacccaac gctgcccga
//
