LOCUS       V004950                 6270 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004950
VERSION     V004950
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Random mutagenesis of Luciola mingrelica firefly luciferase. Mutant
            enzymes with bioluminescence spectra showing low pH sensitivity
  JOURNAL   Biochemistry Mosc. 73 (8), 862-869 (2008)
   PUBMED   18774932
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Koksharov MI, Ugarova NN.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2010) Department of Chemistry, Lomonosov Moscow
            State University, Leninskie Gory, Bld. 1/11, GSP-3, Moscow 119991,
            Russia
REFERENCE   3  (bases 1 to 6270)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6270)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Biochemistry Mosc."; date: "2008"; volume: "73"; issue: "8"; pages:
            "862-869"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JUL-2010) Department of Chemistry, Lomonosov Moscow State
            University, Leninskie Gory, Bld. 1/11, GSP-3, Moscow 119991, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(8..757)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             976..1554
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     CDS             1615..3258
                     /note="Luciferin 4-monooxygenase from Luciola mingrelica.
                     Accession#: Q26304"
                     /label="Luciferin 4-monooxygenase"
     promoter        3436..3540
                     /label="AmpR promoter"
     CDS             3541..4398
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4572..5160
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(5346..5486)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(5591..5779)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
ORIGIN
        1 gtacttaact tttaaagtat gggcaatcaa ttgctcctgt taaaattgct ttagaaatac
       61 tttggcagcg gtttgttgta ttgagtttca tttgcgcatt ggttaaatgg aaagtgaccg
      121 tacgcttact gcagcctaat atttttgaaa tatcccaaga gctctttcct tcgcatgccc
      181 acgctaaaca ttctttttct cttttggtta aatcgttgtt tgatttatta tttgctatat
      241 ttatttttcg ataattatca actagagaag gaacaattaa tggtatgttc atacatgcat
      301 gtaaaaataa actatctata tagttgtctt tctctgaatg tgcaaaacta agcattccga
      361 agccattatt agcagtatga atagggaaac taaacccagt gataagacct gatgatttcg
      421 cttctttaat tacatttgga gattttttat ttacagcatt gttttcaaat atattccaat
      481 taattggtga atgattggag ttagaataat ctactatagg atcatatttt attaaattag
      541 cgtcatcata atattgcctc cattttttag ggtaattatc tagaattgaa atatcagatt
      601 taaccatgga atgaggataa atgatcgcga gtaaataata ttcacaatgt accattttag
      661 tcatatcaga taagcattga ttaatatcat tattgcttct acaagcttta attttattaa
      721 ttattctgta agtgtcgtcg gcatttatgt ctttcatacc catctcttta tccttaccta
      781 ttgtttgtcg caagttttgc gtgttatata tcattaaaac ggtaatagat tgacatttga
      841 ttctaataaa ttggattttt gtcacactat tatatcgctt gaaatacaat tgtttaacat
      901 aagtacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag tcgattaaac
      961 gcaagggagg ttggtatgac tataatgata aaaaaatcgg attttttggc aattccatcg
     1021 gaggagtata aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag acttgagtgg
     1081 gacttagttg tagaaaataa ccttgaatca gatgagtatg ataactcaaa tgcagaatat
     1141 atttatgctt gtgatgatac tgaaaatgta agtggatgct ggcgtttatt acctacaaca
     1201 ggtgattata tgctgaaaag tgtttttcct gaattgcttg gtcaacagag tgctcccaaa
     1261 gatcctaata tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc caagataaat
     1321 aactctgcta gtgaaattac aatgaaacta tttgaagcta tatataaaca cgctgttagt
     1381 caaggtatta cagaatatgt aacagtaaca tcaacagcaa tagagcgatt tttaaagcgt
     1441 attaaagttc cttgtcatcg tattggagac aaagaaattc atgtattagg tgatactaaa
     1501 tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt aaattaatat
     1561 tgttaaatca ttaatttatt ttaaatacta agtatattat aggaggctag caaaatggaa
     1621 atggaaaagg aggagaatgt tgtatatggc cctctgccat tctaccccat tgaagaagga
     1681 tcagctggaa ttcagttgca taagtacatg catcaatatg ccaaacttgg agcaattgct
     1741 ttttcgaacg cccttactgg agtcgacatt tcttaccaag aatactttga tattacatgt
     1801 cgtttagctg aggccatgaa aaactttggt atgaaaccgg aagaacatat tgctttgtgc
     1861 agtgaaaatt gtgaagaatt tttcatccct gtacttgctg gtctttacat tggggtagct
     1921 gttgcaccta ctaatgaaat ttacacattg cgtgaactta atcatagttt gggcatcgca
     1981 caaccaacta ttgtattcag ctcgagaaaa ggcttaccta aagttttaga agtgcaaaaa
     2041 acagttacat gcatcaaaaa aattgttatt ttagatagta aagtaaactt tgggggccac
     2101 gattgtatgg aaacttttat taagaaacat gtagaattag gttttcaacc aagtagcttt
     2161 gtacccattg atgtaaagaa ccgtaaacaa cacgttgctt tgcttatgaa ttcttctggc
     2221 tctactggtt tacctaaagg tgtacgaatt acccacgaag gtgcagttac aagattctca
     2281 cacgctaagg atccaattta cggaaaccaa gtttcacctg gtactgctat tttaactgtc
     2341 gttccgttcc atcatggatt tggaatgttt accactttag gatactttgc ttgtggatac
     2401 cgtgttgtaa tgttaacaaa atttgatgaa gaactatttt tgagaacttt gcaagattat
     2461 aagtgtacca gtgttattct ggtaccaacg ttatttgcta ttctcaacaa gagtgaattg
     2521 atcgataagt tcgatttatc taatctaact gaaattgctt ctggtggagc tcctttggca
     2581 aaagaagttg gcgaagcagt cgctagaaga tttaatctac ccggtgtccg tcagggttac
     2641 ggattaacag aaacgacatc tgcatttatt attaccccag aaggtgatga taaacctgga
     2701 gcatctggaa aagtagtacc cttattcaaa gtaaaagtta ttgatcttga cactaaaaaa
     2761 actttgggtg tcaaccgacg aggagagatc tgtgtaaaag gacccagtct tatgttaggc
     2821 tactcgaaca atccggaagc aacaagagaa actattgatg aagagggttg gttgcacaca
     2881 ggagatattg gatattacga cgaagacgaa catttcttca ttgtcgatcg tttgaaatca
     2941 ttaatcaaat acaaggggta ccaggtacca cctgctgaat tggaatccgt tcttttgcaa
     3001 catccaaata tatttgatgc tggtgtggct ggtgtccccg atcccgatgc tggcgaactt
     3061 ccaggggctg tagttgtaat ggaaaaagga aaaactatga ctgaaaagga aattgtggat
     3121 tatgttaata gtcaagtagt gaaccacaaa cgtctgcgtg gtggcgttcg ttttgtggat
     3181 gaagtaccta aaggtctaac tggtaaaatt gatgctaaag taattagaga aattcttaag
     3241 aaaccacaag ccaagatgtg atcctctaga gtcgatgact tgattttcat gggaactcct
     3301 cagcaatggt cattgcagga acaaaaacag aattcttgaa gacgaaaggg cctcgtgata
     3361 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     3421 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3481 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3541 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3601 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3661 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3721 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3781 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3841 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3901 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3961 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4021 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4081 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4141 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4201 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     4261 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     4321 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     4381 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     4441 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     4501 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     4561 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     4621 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     4681 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     4741 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     4801 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     4861 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     4921 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     4981 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5041 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5101 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5161 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     5221 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     5281 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     5341 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     5401 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     5461 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     5521 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     5581 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     5641 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     5701 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     5761 ttttttcctg tttggtcact tgatgcctcc gtgtaagggg gaatttctgt tcatgggggt
     5821 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc
     5881 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag
     5941 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg
     6001 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg
     6061 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc
     6121 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta
     6181 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg
     6241 cacccgtggc caggacccaa cgctgcccga
//
