LOCUS       62056_14370        5523 bp DNA     circular SYN 20-JUL-2019
DEFINITION  Shuttle vector pLPV3Z, complete sequence.
ACCESSION   MK681508
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5523)
  AUTHORS   Visca P.
  TITLE     New shuttle vectors for real-time gene expression analysis in 
            multidrug-resistant Acinetobacter species: in vitro and in vivo 
            applications
  JOURNAL   Appl. Environ. Microbiol. (2019) In press
REFERENCE   2  (bases 1 to 5523)
  AUTHORS   Lucidi M, Prencipe E, Visca P.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2019) Department of Science, Roma Tre University, 
            Viale Marconi 446, Rome, Roma 00146, Italia
REFERENCE   3  (bases 1 to 5523)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-MAR-2019) Department of Science, Roma Tre University, Viale 
            Marconi 446, Rome, Roma 00146, Italia"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      194..782
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        974..992
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1022..1038
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1072..1088)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1097..1107
                     /label=stop codons in all the three reading frames
                     /note="stop codons in all the three reading frames"
     regulatory      1110..1121
                     /label=optimized RBS
                     /note="optimized RBS"
                     /regulatory_class="ribosome_binding_site"
     CDS             1128..1841
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     terminator      1864..1958
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     regulatory      2076..2104
                     /label=ble gene predicted promoter
                     /note="ble gene predicted promoter"
                     /regulatory_class="promoter"
     CDS             2149..2520
                     /label=BleoR
                     /note="antibiotic-binding protein"
     promoter        complement(2622..2640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2650..2666)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2905..3009
                     /label=AmpR promoter
     promoter        3027..3055
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3244..3774
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     regulatory      3937..3964
                     /label=putative antitoxin-toxin module-predicted promoter
                     /note="putative antitoxin-toxin module-predicted promoter"
                     /regulatory_class="promoter"
     CDS             3998..4291
                     /codon_start=1
                     /transl_table=11
                     /product="putative PaaA2-like antitoxin"
                     /label=putative PaaA2-like antitoxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="QDL88250.1"
                     /translation="MTEATFTFRVDHDLKQEFSSLAKTVDRSGAQLIRDFMRDFVKKQQ
                     EAADYDKWFKQQVQIGLNEANAGKLIPHEDVKAEFAARRAATLAKLAAKNED"
     CDS             4281..4550
                     /codon_start=1
                     /transl_table=11
                     /product="putative ParE2-like toxin"
                     /label=putative ParE2-like toxin
                     /note="putative component of an antitoxin-toxin system
                     involved in plasmid stability and maintenance"
                     /protein_id="QDL88251.1"
                     /translation="MKIEWTETARQDRRNIYDYLEERNPIAAIEIDDLIEEKTDLLVDN
                     RLMGRTGRQKDTRELVIHPHYVVVYDITDIIRILRVLHTSQEWS"
     regulatory      4551..5522
                     /note="minimal origin of replication for Acinetobacter
                     spp."
                     /regulatory_class="replication_regulatory_region"
ORIGIN
        1 tgcatgtcta cggcatcctc caggggggat cacaagttta ctcatatata ctttagattg
       61 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
      121 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
      181 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      241 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      301 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      361 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      421 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      481 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      541 tggagcgaac gacctacacc gaacagagat acctacagcg tgagctatga gaaagcgcca
      601 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      661 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      721 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      781 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      841 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      901 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      961 aagagcgccc agcaattaac cctcactaaa gggaacaaaa gctgggtacc gggccccccc
     1021 tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg ggatccacta
     1081 gttctagagc ggccgctaag taagtaatta aagaggagaa attaagcatg cgtaaaggag
     1141 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
     1201 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
     1261 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
     1321 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
     1381 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
     1441 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     1501 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
     1561 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
     1621 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     1681 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     1741 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     1801 cagctgctgg gattacacat ggcatggatg aactatacaa ataagcttaa ttagctgagc
     1861 ttggactcct gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa
     1921 cgctcggttg ccgccgggcg ttttttattg gtgagaatcc aagctagctt gagctcttct
     1981 ccttacgcat ctgtgcggta tttcacaccg catacaggtg gcacttttcg gggaaatgtg
     2041 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     2101 caataaccct gataaatgct tcaataatag cacgtgagga gggccaccat ggccaagttg
     2161 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     2221 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     2281 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     2341 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     2401 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     2461 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     2521 tgacacatgc ataccaagta tgcagcccct gctgctgttg ctgctgttca agtgtcaatt
     2581 gctctactct gttgctcgtc tcaatgagag ctccaattcg ccctatagtg agtcgtatta
     2641 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     2701 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     2761 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaaat tgtaaccatg
     2821 gtcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     2881 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     2941 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3001 aggaagagta tgagtattca accgaattga cataagcctg ttcggttcgt aaactgtaat
     3061 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     3121 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttgtacag tctatgcctc
     3181 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
     3241 acgatgttac gcagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     3301 ggtggctcaa gtatgggcat cattcgcaca tgtaggctcg gccctgacca agtcaaatcc
     3361 atgcgggctg ctcttgatct tttcggtcgt gagttcggag acgtagccac ctactcccaa
     3421 catcagccgg actccgatta cctcgggaac ttgctccgta gtaagacatt catcgcgctt
     3481 gctgccttcg accaagaagc ggttgttggc gctctcgcgg cttacgttct gcccaagttt
     3541 gagcagccgc gtagtgagat ctatatctat gatctcgcag tctccggcga gcaccggagg
     3601 cagggcattg ccaccgcgct catcaatctc ctcaagcatg aggccaacgc gcttggtgct
     3661 tatgtgatct acgtgcaagc agattacggt gacgatcccg cagtggctct ctatacaaag
     3721 ttgggcatac gggaagaagt gatgcacttt gatatcgacc caagtaccgc cacctaacaa
     3781 ttcgttcaag ccgagatcgg cttcccggcc gcggagttgt tcggtaaatt gtcacaacgc
     3841 cgcggccaat tcgcatcgat tgtcagacca agtatgcagc ccctgctgct gttgctgctg
     3901 atgcatacca agaacagcct aagccgaaaa aagacaatga cctaactttt taatgtgtta
     3961 caatagcatt caaatgtaac acattggagt gaaaaacatg acagaagcga cttttacctt
     4021 tcgggtagac catgacctaa agcaagaatt ttcaagcctt gctaagactg ttgatcggtc
     4081 gggggcgcag ctaatccgtg acttcatgcg tgactttgtt aagaagcaac aggaagccgc
     4141 agactatgac aaatggttca agcagcaggt acagatcggc ttaaatgaag ccaatgcagg
     4201 caagttgatt ccacatgagg acgtaaaggc agagtttgcc gctagacgtg ctgcaacatt
     4261 ggcgaaactg gcagcgaaga atgaagattg agtggactga aacagctcgc caagatcgta
     4321 gaaatatcta tgattatctt gaagaacgca accctatagc agctattgaa attgatgatt
     4381 taattgaaga aaagacagat ttacttgttg ataatcgact gatggggcgc acaggcagac
     4441 agaaagatac tagggagtta gtgatacatc cgcattatgt ggttgtatat gacatcactg
     4501 atataatacg gatactcaga gtgctacaca catcgcagga gtggtcatga cttactcatg
     4561 tactttggat tatttagtgt tataaaatcc tgatttataa attttttttg ttaaaaaaga
     4621 taaaagcccc ttgcaattgc ttggggcttt accgtaattt atggggtaca gatcttcgat
     4681 actgacatat cggcaatcga aagcattaag gtttgacgac cgctaatgat ttcaccacag
     4741 gggcttaatg tacctgtctt aaattctaag gttttaactc gctttgtcaa gcatagaccc
     4801 caaaaattta gccaatgtct gtaactcaat ctgtccatgt gtgggtgatg aggtacagtg
     4861 acgctagcac acatcggaaa aacgctatta ctaggggaac tgaacagagt agcggacgca
     4921 atgagtagtc atttaattgg cggttatgag cgtgttcagg cggtgctatc aatcgtaatc
     4981 ataacagtgg cagcttgata cagtgatgtc atccctgatg cgaaagcgac cgaccgacgg
     5041 tacatcgaat gggaatactt tagggtgatt tttaagaatc gctctagggt gagtatttcc
     5101 cattcagctc tgctccctcc ctctggtact ttaatcaaaa gcactactaa acatatgttt
     5161 ttaaataaaa aatattgata tagagataat attagtaaga ataattaaac aattgaatat
     5221 agataaatca ttgttaaata aagattaatt attaaaatga atgtatactt atatataaat
     5281 caatgattta aaatatttga taaagaaaac ttttcaaaaa aaatataatt gagattgtgt
     5341 catttcggtc aattcttaat atgttccacg caagttttag ctatggtgct aaacagaaat
     5401 ttgctgaaaa agaacttttc actgaactgg ttaaaatgta agcagcctga gagccgccaa
     5461 aaattttaaa aacaaaccgc cttaatcatc ttcaaaaaat acctctaaaa cctcaccatt
     5521 tga
//
