LOCUS       V000813                 8743 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V000813
VERSION     V000813
KEYWORDS    pLPCX2-MT1-MMP-eGFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Sun J, Lu F, He H, Shen J, Messina J, Mathew R, Wang D, Sarnaik AA,
            Chang WC, Kim M, Cheng H, Yang S
  TITLE     STIM1- and Orai1-mediated Ca(2+) oscillation orchestrates
            invadopodium formation and melanoma invasion.
  JOURNAL   J Cell Biol. 2014 Nov 24;207(4):535-48. doi: 10.1083/jcb.201407082.
            Epub 2014 Nov 17.
   PUBMED   25404747
REFERENCE   2  (bases 1 to 8743)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8743)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi:
            "10.1083/jcb.201407082"; journalName: "J Cell Biol"; date:
            "2014-11-24- 24"; volume: "207"; issue: "4"; pages: "535-48"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             583..2328
                     /gene="MMP14"
                     /label="Matrix metalloproteinase-14"
                     /note="Matrix metalloproteinase-14 from Homo sapiens.
                     Accession#: P50281"
     CDS             2359..3075
                     /label="EGFP"
                     /note="enhanced GFP"
     LTR             3144..3737
                     /label="LTR"
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     primer_bind     complement(3840..3862)
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     protein_bind    3990..4023
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(4083..4116)
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     primer_bind     4136..4155
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     complement(4236..4253)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4407..4995)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5169..6026)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6027..6131)
                     /label="AmpR promoter"
     primer_bind     6199..6217
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     LTR             6407..6994
                     /label="5' LTR"
                     /note="long terminal repeat from Moloney murine sarcoma
                     virus"
     misc_feature    7057..7256
                     /label="MMLV Psi"
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             7457..7873
                     /label="gag (truncated)"
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     CDS             7970..8566
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     promoter        8687..8743
                     /label="CMV"
                     /note="Human cytomegalovirus immediate early
                     enhancer/promoter"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttgga gtttctcgga ccatgtctcc cgccccaaga
      601 cccccccgtt gtctcctgct ccccctgctc acgctcggca ccgcgctcgc ctccctcggc
      661 tcggcccaaa gcagcagctt cagccccgaa gcctggctac agcaatatgg ctacctgcct
      721 cccggggacc tacgtaccca cacacagcgc tcaccccagt cactctcagc ggccatcgct
      781 gccatgcaga agttttacgg cttgcaagta acaggcaaag ctgatgcaga caccatgaag
      841 gccatgaggc gcccccgatg tggtgttcca gacaagtttg gggctgagat caaggccaat
      901 gttcgaagga agcgctacgc catccagggt ctcaaatggc aacataatga aatcactttc
      961 tgcatccaga attacacccc caaggtgggc gagtatgcca catacgaggc cattcgcaag
     1021 gcgttccgcg tgtgggagag tgccacacca ctgcgcttcc gcgaggtgcc ctatgcctac
     1081 atccgtgagg gccatgagaa gcaggccgac atcatgatct tctttgccga gggcttccat
     1141 ggcgacagca cgcccttcga tggtgagggc ggcttcctgg cccatgccta cttcccaggc
     1201 cccaacattg gaggagacac ccactttgac tctgccgagc cttggactgt caggaatgag
     1261 gatctgaatg gaaatgacat cttcctggtg gctgtgcacg agctgggcca tgccctgggg
     1321 ctcgagcatt ccagtgaccc ctcggccatc atggcaccct tttaccagtg gatggacacg
     1381 gagaattttg tgctgcccga tgatgaccgc cggggcatcc agcaacttta tgggggtgag
     1441 tcagggttcc ccaccaagat gccccctcaa cccaggacta cctcccggcc ttctgttcct
     1501 gataaaccca aaaaccccac ctatgggccc aacatctgtg acgggaactt tgacaccgtg
     1561 gccatgctcc gaggggagat gtttgtcttc aaggagcgct ggttctggcg ggtgaggaat
     1621 aaccaagtga tggatggata cccaatgccc attggccagt tctggcgggg cctgcctgcg
     1681 tccatcaaca ctgcctacga gaggaaggat ggcaaattcg tcttcttcaa aggagacaag
     1741 cattgggtgt ttgatgaggc gtccctggaa cctggctacc ccaagcacat taaggagctg
     1801 ggccgagggc tgcctaccga caagattgat gctgctctct tctggatgcc caatggaaag
     1861 acctacttct tccgtggaaa caagtactac cgtttcaacg aagagctcag ggcagtggat
     1921 agcgagtacc ccaagaacat caaagtctgg gaagggatcc ctgagtctcc cagagggtca
     1981 ttcatgggca gcgatgaagt cttcacttac ttctacaagg ggaacaaata ctggaaattc
     2041 aacaaccaga agctgaaggt agaaccgggc taccccaagt cagccctgag ggactggatg
     2101 ggctgcccat cgggaggccg gccggatgag gggactgagg aggagacgga ggtgatcatc
     2161 attgaggtgg acgaggaggg cggcggggcg gtgagcgcgg ctgccgtggt gctgcccgtg
     2221 ctgctgctgc tcctggtgct ggcggtgggc cttgcagtct tcttcttcag acgccatggg
     2281 acccccaggc gactgctcta ctgccagcgt tccctgctgg acaaggtccc gcgggcccgg
     2341 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2401 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     2461 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2521 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     2581 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2641 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2701 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2761 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     2821 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     2881 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2941 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     3001 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     3061 gacgagctgt acaagtaaag cggccgcctc ggccaaacat cgataaaata aaagatttta
     3121 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct
     3181 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga
     3241 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
     3301 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg
     3361 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     3421 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     3481 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     3541 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt
     3601 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     3661 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     3721 agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac
     3781 cgacccacca ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     3841 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     3901 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     3961 cagtcacgta gcgatagcgg agtgtactga taacttcgta taatgtatgc tatacgaagt
     4021 tattaggtct gaagaggagt ttacgtccag ccaagctagg gccgcgatcc ggaaccctta
     4081 atataacttc gtataatgta tgctatacga agttatcagt actggcttaa ctatgcggca
     4141 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     4201 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4381 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4741 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4801 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     4861 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4921 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4981 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5041 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5101 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5161 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5221 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5281 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5341 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5401 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5461 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5521 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5581 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5641 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5701 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5761 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     5821 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5881 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5941 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6001 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6061 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     6121 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     6181 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattgcta
     6241 gcaattgcta gcaattgcta gcaattcata ccagatcacc gaaaactgtc ctccaaatgt
     6301 gtccccctca cactcccaaa ttcgcgggct tctgcctctt agaccactct accctattcc
     6361 ccacactcac cggagccaaa gccgcggccc ttccgtttct ttgcttttga aagaccccac
     6421 ccgtaggtgg caagctagct taagtaacgc cactttgcaa ggcatggaaa aatacataac
     6481 tgagaataga aaagttcaga tcaaggtcag gaacaaagaa acagctgaat accaaacagg
     6541 atatctgtgg taagcggttc ctgccccggc tcagggccaa gaacagatga gacagctgag
     6601 tgatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcgg ggccaagaac
     6661 agatggtccc cagatgcggt ccagccctca gcagtttcta gtgaatcatc agatgtttcc
     6721 agggtgcccc aaggacctga aaatgaccct gtaccttatt tgaactaacc aatcagttcg
     6781 cttctcgctt ctgttcgcgc gcttccgctc tccgagctca ataaaagagc ccacaacccc
     6841 tcactcggcg cgccagtctt ccgatagact gcgtcgcccg ggtacccgta ttcccaataa
     6901 agcctcttgc tgtttgcatc cgaatcgtgg tctcgctgtt ccttgggagg gtctcctctg
     6961 agtgattgac tacccacgac gggggtcttt catttggggg ctcgtccggg atttggagac
     7021 ccctgcccag ggaccaccga cccaccaccg ggaggtaagc tggccagcaa cttatctgtg
     7081 tctgtccgat tgtctagtgt ctatgtttga tgttatgcgc ctgcgtctgt actagttagc
     7141 taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa cacccggccg
     7201 caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga cctgaggaag
     7261 ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag acgagaacct
     7321 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc
     7381 ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg tgtttctgta
     7441 tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct taggtcactg
     7501 gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga gacgttgggt
     7561 taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag acggcacctt
     7621 taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc cgcatggaca
     7681 cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc cccctccctg
     7741 ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg ccccgtctct
     7801 cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag ccctcactcc
     7861 ttctctaggc gccggaatga tcccccgggc tgcaggtcgg ccgccacgac cggtgccgcc
     7921 accatcccct gacccacgcc cctgacccct cacaaggaga cgaccttcca tgaccgagta
     7981 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
     8041 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
     8101 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     8161 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     8221 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     8281 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     8341 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     8401 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     8461 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     8521 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
     8581 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat ggctccgacc gaagccaccc
     8641 ggggcggccc cgccgacccc gcacccgccc ccgaggccca ccgactctag taatagtaat
     8701 caattacggg gtcattagtt catagcccat atatggagtt ccg
//
