LOCUS       Exported                3885 bp DNA     circular SYN 05-AUG-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3885)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             18..617
                     /codon_start=1
                     /label=vmi480
                     /translation="MMTKKPEFYAPDLTEERLHILSENLLDVLDEAHHYSESPNATAWF
                     KGTANYGLPQGMLIRMHSDSAYPWLTLANQTMDYTARVGNTLVQFVVDDPHSPRKQHRL
                     KRNAVEKHQISLELEEDYVDTPLIWRFYLNPISNGVDYSPSISLLGFNGNGNVICSWEY
                     DTVVTGPVVTDKPESVEIDEPLLVRKKKVQKKVSDE"
     promoter        complement(712..730)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      745..1133
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(1258..1917)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1918..2020)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     promoter        2147..2165
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     oriT            2319..2442
                     /label=incP origin of transfer
                     /note="incP origin of transfer"
     CDS             complement(2682..3560)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        3587..3871
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 aagaaggaga tatacatatg atgaccaaaa aacctgaatt ttatgctcca gacctaacgg
       61 aagaacgctt gcacatattg agtgaaaacc tactcgatgt gctcgatgag gcccatcatt
      121 actcagaaag tcccaatgcg acggcctggt ttaaaggcac tgcaaattat ggtttaccac
      181 agggaatgct aatccgtatg cactcagaca gtgcttatcc atggctcaca cttgccaacc
      241 aaacaatgga ttacactgct agagttggaa acactcttgt tcaatttgtt gtggatgacc
      301 ctcactcacc tcgcaagcaa catcgcctta aacgcaatgc tgttgaaaag catcaaatat
      361 ctcttgaatt agaggaagac tatgttgata ccccattgat ttggcgattt tacttaaatc
      421 ctatatctaa tggtgttgat tattccccat ctatttcttt gttgggtttc aacggcaatg
      481 gcaacgttat atgcagttgg gaatacgaca cagttgtaac tggtccagtt gttacagaca
      541 aaccagaatc ggttgaaatt gatgagccat tattggttcg taaaaagaaa gtacaaaaaa
      601 aggtctctga tgagtaaaag ctcgagggaa tataagtcga cggatccccc gggctgcagg
      661 aatctagacc ttgagtcgaa ttcgagctcg acatatcgtc gctagctgtt ccctttagtg
      721 agggttaatt gcgcgcaatt cccatgtcag ccgttaagtg ttcctgtgtc actcaaaatt
      781 gctttgagag gctctaaggg cttctcagtg cgttacatcc ctggcttgtt gtccacaacc
      841 gttaaacctt aaaagcttta aaagccttat atattctttt ttttcttata aaacttaaaa
      901 ccttagaggc tatttaagtt gctgatttat attaatttta ttgttcaaac atgagagctt
      961 agtacgtgaa acatgagagc ttagtacgtt agccatgaga gcttagtacg ttagccatga
     1021 gggtttagtt cgttaaacat gagagcttag tacgttaaac atgagagctt agtacgtgaa
     1081 acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatcttcag atcctctacg
     1141 ccggacgcat cgtggccgga tcagatctga tatcgtcgca gaccaaaacg atctcaagaa
     1201 gatcatctta ttaatcagat aaaatatttc taggcaccaa taactgcctt aaaaaaatta
     1261 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     1321 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     1381 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     1441 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
     1501 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     1561 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     1621 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     1681 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
     1741 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     1801 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     1861 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     1921 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     1981 ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt ttcgccaaaa
     2041 gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt ctgcgaagtg
     2101 atcttccgtc acaggtattt attcggcgca attgatatcg cgcgcgtaat acgactcact
     2161 atagggcgaa ttgggtacca gcgcttttcc gctgcataac cctgcttcgg ggtcattata
     2221 gcgatttttt cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt
     2281 cgtgtagact ttccttggtg tatccaacgg cgtcagccgg gcggcgtcag ccgggcagga
     2341 taggtgaagt aggcccaccc gcgagcgggt gttccttctt cactgtccct tattcgcacc
     2401 tggcggtgct caacgggaat cctgctctgc gaggctggcc ggcgtcgacg gtatcgataa
     2461 gcttggcatg cctgcagcat acactccgct atcgctacgt gactgggtca tggctgcgcc
     2521 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     2581 ttacagatcg atgcataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta
     2641 tgctactccg tcaagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct
     2701 acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat
     2761 tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg
     2821 gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg ttggtcctcg
     2881 cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc
     2941 gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg
     3001 ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag cgactcgtta
     3061 atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa
     3121 tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc
     3181 tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg ccagttaagc
     3241 cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc
     3301 tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt
     3361 cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg
     3421 agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc
     3481 tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg cagcagggga
     3541 tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt
     3601 ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa
     3661 ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa
     3721 acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc
     3781 gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc
     3841 tttttatcgc aactctctac tgtttctcca tacccgtttt tttgg
//
