LOCUS       Exported                5823 bp DNA     circular SYN 02-SEP-2024
DEFINITION  Lentiviral packaging plasmid for expression of the vesicular 
            stomatitis virus G glycoprotein.
ACCESSION   .
VERSION     .
KEYWORDS    pLP VSVG
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5823)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5823)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5823)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        79..458
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        459..662
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          796..1271
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             1346..2878
                     /label=VSV-G
                     /note="vesicular stomatitis virus G glycoprotein"
     polyA_signal    3083..3477
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     rep_origin      complement(3987..4575)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4749..5606)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5607..5711)
                     /label=AmpR promoter
ORIGIN
        1 ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
       61 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      121 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      181 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      241 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      301 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      361 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      421 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      481 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      541 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      601 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      661 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      721 gaagacaccg ggaccgatcc agcctcccct cgaagcttac atgtggtacc gagctcggat
      781 cctgagaact tcagggtgag tctatgggac ccttgatgtt ttctttcccc ttcttttcta
      841 tggttaagtt catgtcatag gaaggggaga agtaacaggg tacacatatt gaccaaatca
      901 gggtaatttt gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata cttttttgtt
      961 tatcttattt ctaatacttt ccctaatctc tttctttcag ggcaataatg atacaatgta
     1021 tcatgcctct ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggcaatag
     1081 caatatttct gcatataaat atttctgcat ataaattgta actgatgtaa gaggtttcat
     1141 attgctaata gcagctacaa tccagctacc attctgcttt tattttatgg ttgggataag
     1201 gctggattat tctgagtcca agctaggccc ttttgctaat catgttcata cctcttatct
     1261 tcctcccaca gctcctgggc aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag
     1321 cacgtgagat ctgaattctg acactatgaa gtgccttttg tacttagcct ttttattcat
     1381 tggggtgaat tgcaagttca ccatagtttt tccacacaac caaaaaggaa actggaaaaa
     1441 tgttccttct aattaccatt attgcccgtc aagctcagat ttaaattggc ataatgactt
     1501 aataggcaca gccttacaag tcaaaatgcc caagagtcac aaggctattc aagcagacgg
     1561 ttggatgtgt catgcttcca aatgggtcac tacttgtgat ttccgctggt atggaccgaa
     1621 gtatataaca cattccatcc gatccttcac tccatctgta gaacaatgca aggaaagcat
     1681 tgaacaaacg aaacaaggaa cttggctgaa tccaggcttc cctcctcaaa gttgtggata
     1741 tgcaactgtg acggatgccg aagcagtgat tgtccaggtg actcctcacc atgtgctggt
     1801 tgatgaatac acaggagaat gggttgattc acagttcatc aacggaaaat gcagcaatta
     1861 catatgcccc actgtccata actctacaac ctggcattct gactataagg tcaaagggct
     1921 atgtgattct aacctcattt ccatggacat caccttcttc tcagaggacg gagagctatc
     1981 atccctggga aaggagggca cagggttcag aagtaactac tttgcttatg aaactggagg
     2041 caaggcctgc aaaatgcaat actgcaagca ttggggagtc agactcccat caggtgtctg
     2101 gttcgagatg gctgataagg atctctttgc tgcagccaga ttccctgaat gcccagaagg
     2161 gtcaagtatc tctgctccat ctcagacctc agtggatgta agtctaattc aggacgttga
     2221 gaggatcttg gattattccc tctgccaaga aacctggagc aaaatcagag cgggtcttcc
     2281 aatctctcca gtggatctca gctatcttgc tcctaaaaac ccaggaaccg gtcctgcttt
     2341 caccataatc aatggtaccc taaaatactt tgagaccaga tacatcagag tcgatattgc
     2401 tgctccaatc ctctcaagaa tggtcggaat gatcagtgga actaccacag aaagggaact
     2461 gtgggatgac tgggcaccat atgaagacgt ggaaattgga cccaatggag ttctgaggac
     2521 cagttcagga tataagtttc ctttatacat gattggacat ggtatgttgg actccgatct
     2581 tcatcttagc tcaaaggctc aggtgttcga acatcctcac attcaagacg ctgcttcgca
     2641 acttcctgat gatgagagtt tattttttgg tgatactggg ctatccaaaa atccaatcga
     2701 gcttgtagaa ggttggttca gtagttggaa aagctctatt gcctcttttt tctttatcat
     2761 agggttaatc attggactat tcttggttct ccgagttggt atccatcttt gcattaaatt
     2821 aaagcacacc aagaaaagac agatttatac agacatagag atgaaccgac ttggaaagta
     2881 actcaaatcc tgcacaacag attcttcatg tttggaccaa atcaacttgt gataccatgc
     2941 tcaaagaggc ctcaattata tttgagtttt taatttttat gaaaaaaaaa aaaaaaaaac
     3001 ggaattcacc ccaccagtgc aggctgccta tcagaaagtg gtggctggtg tggctaatgc
     3061 cctggcccac aagtatcact aagctcgctt tcttgctgtc caatttctat taaaggttcc
     3121 tttgttccct aagtccaact actaaactgg gggatattat gaagggcctt gagcatctgg
     3181 attctgccta ataaaaaaca tttattttca ttgcaatgat gtatttaaat tatttctgaa
     3241 tattttacta aaaagggaat gtgggaggtc agtgcattta aaacataaag aaatgaagag
     3301 ctagttcaaa ccttgggaaa atacactata tcttaaactc catgaaagaa ggtgaggctg
     3361 caaacagcta atgcacattg gcaacagccc ctgatgccta tgccttattc atccctcaga
     3421 aaaggattca agtagaggct tgatttggag gttaaagttt tgctatgctg tattttacat
     3481 tacttattgt tttagctgtc ctcatgaatg tcttttcact acccatttgc ttatcctgca
     3541 tctctcagcc ttgactccac tcagttctct tgcttagaga taccaccttt cccctgaagt
     3601 gttccttcca tgttttacgg cgagatggtt tctcctcgcc tggccactca gccttagttg
     3661 tctctgttgt cttatagagg tctacttgaa gaaggaaaaa cagggggcat ggtttgactg
     3721 tcctgtgagc ccttcttccc tgcctccccc actcacagtg acccggaatc cctcgacatg
     3781 gcagtctagc actagtgcgg ccgcagatct gcttcctcgc tcactgactc gctgcgctcg
     3841 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3901 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3961 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4021 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4081 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4141 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4201 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4261 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4321 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4381 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4441 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4501 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4561 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4621 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4681 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4741 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4801 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4861 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4921 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4981 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5041 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5101 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5161 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5221 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5281 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5341 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5401 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5461 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5521 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5581 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5641 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5701 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tggatcccct gagggggccc
     5761 ccatgggcta gaggatccgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     5821 agc
//
