LOCUS       40924_28581        4156 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant expression vector pLOLA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ferrando A, Koncz-Kalman Z, Farras R, Tiburcio A, Schell J, Koncz C.
  TITLE     Detection of in vivo protein interactions between Snf1-related 
            kinase subunits with intron-tagged epitope-labelling in plants cells
  JOURNAL   Nucleic Acids Res. 29 (17), 3685-3693 (2001)
  PUBMED    11522840
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Berendzen KW, Koncz C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2004) Development, Max-Planck Institute for Plant 
            Breeding Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany
REFERENCE   3  (bases 1 to 4156)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4156)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001"; volume: "29"; issue: "17"; pages: 
            "3685-3693"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-2004) Development, Max-Planck Institute for Plant Breeding 
            Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..18
                     /note="Tth111I, NotI, XhoI, KpnI; MCS promoter"
     promoter        409..754
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    759..793
                     /note="HindIII, NcoI, SmaI, XbaI, BglII; MCS upstream"
     misc_feature    794..808
                     /label=N-term c-myc epitope
                     /note="N-term c-myc epitope"
     intron          809..997
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     misc_feature    998..1012
                     /label=C-term c-myc epitope
                     /note="C-term c-myc epitope"
     misc_feature    1013..1040
                     /note="SalI, BamHI, PstI, EcoRI; MCS downstream"
     regulatory      1041..1293
                     /note="polyadenylation signal from nopaline synthase gene
                     of the Agrobacterium Ti plasmid; NOS terminator"
                     /regulatory_class="terminator"
     promoter        complement(1325..1343)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1373..1389)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1397..1413)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1421..1451)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1466..1487)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1775..2363)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2537..3394)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3395..3499)
                     /label=AmpR promoter
     rep_origin      3526..3981
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4122..4138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcggccgctc gaggtacccc tactccaaaa atgtcaaaga tacagtctca ntaagnacca
       61 aagggctatt gagacttttc aacaaagggt aatttcggga aacctcctcg gattccattg
      121 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
      181 ccatcattgc gataaaggaa aggctatcat tcaagatgcc tctgccgaca gtggtcccaa
      241 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      301 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccaccc
      361 ctactccaaa aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca
      421 acaaagggta atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
      481 cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
      541 ggctatcatt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
      601 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
      661 catctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
      721 tatataagga agttcatttc atttggagag gacagcccaa gcttccacca tggcgcccgg
      781 gtctagaaga tctgagcaaa agttgattgt aagtttctgc ttctaccttt gatatatata
      841 taataattat cattaattag tagtaatata atattttaaa tatttttttc aaaataaaag
      901 aatgtagtat atagcaattg cttttctgta gtttataagt gtgtatattt caatttataa
      961 cttttctaat atatgaccaa aatttgttga tgtgcagtct gaggaggatc ttgagtcgac
     1021 ggatccactg cagtgaattc tcgttcaaac atttggcaat aaagtttctt aagattgaat
     1081 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
     1141 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     1201 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     1261 tcgcgcgcgg tgtcatctgt cgaggggggg cccggtaccg agctcgcggc cgctcgacga
     1321 gctccctata gtgagtcgta ttanaggccg acttggccaa attcgtaatc atgtcatagc
     1381 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     1441 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     1501 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1561 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1621 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1681 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1741 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1801 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1861 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1921 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1981 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     2041 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     2101 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2161 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     2221 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2281 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2341 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2401 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2461 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2521 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2581 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2641 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     2701 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2761 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2821 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     2881 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2941 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     3001 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     3061 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3121 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3181 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3241 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3301 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3361 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3421 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3481 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
     3541 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3601 ccctagggcc cggtcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3661 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3721 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3781 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3841 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     3901 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3961 aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     4021 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     4081 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
     4141 caagctgact tggtca
//
