LOCUS       40924_28556       10495 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLNMCL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10495)
  AUTHORS   Shahana S, Childers DS, Ballou ER, Bohovych I, Odds FC, Gow NA, 
            Brown AJ.
  TITLE     New Clox Systems for Rapid and Efficient Gene Disruption in Candida 
            albicans
  JOURNAL   PLoS ONE 9 (6), E100390 (2014)
  PUBMED    24940603
REFERENCE   2  (bases 1 to 10495)
  AUTHORS   Shahana S, Childers DS, Brown AJP.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-MAY-2013) Microbiology, School of Medical Sciences, 
            Institute of Medical Sciences - Foresterhill, Aberdeen, 
            Aberdeenshire AB25 2ZD, United Kingdom
REFERENCE   3  (bases 1 to 10495)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10495)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2014"; volume: "9"; issue: "6"; pages: "E100390"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-MAY-2013) Microbiology, School of Medical Sciences, Institute of
            Medical Sciences - Foresterhill, Aberdeen, Aberdeenshire AB25 2ZD, 
            United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(666..1757)
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
     promoter        complement(1770..2174)
                     /label=LEU2 promoter
     rep_origin      complement(2474..2929)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3074..3090
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3097..3115
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3148..3164
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3178..3211
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     terminator      complement(3622..3819)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(3837..4400)
                     /label=NrsR
                     /note="nourseothricin acetyltransferase"
     promoter        complement(4420..4763)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     regulatory      4827..6158
                     /gene="pMET3"
                     /label=MET3 promoter
                     /note="MET3 promoter"
                     /regulatory_class="promoter"
     gene            4827..6158
                     /gene="pMET3"
                     /label=pMET3
     CDS             6186..6593
                     /codon_start=1
                     /product="Cre"
                     /label=Cre
                     /note="Cre recombinase"
                     /protein_id="AGZ63435.1"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQAL"
     protein_bind    complement(7597..7630)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7675..7691)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(7721..7739)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7760..7776)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7784..7800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7808..7838)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7853..7874)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8162..8750)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8924..9781)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9782..9886)
                     /label=AmpR promoter
     misc_feature    9923..10426
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc
      241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc
     1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca
     1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg
     2341 atgtaattgt tccgattcca tttttaataa ggcaataata ttaggtatgt ggatatacta
     2401 gaagttctcc tcgagggtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     3001 cagctggcga aggggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     3061 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
     3121 ttggagctcc accgcggtgg cggccgctct agaactagtg gatccagatc ttgatccata
     3181 acttcgtata gcatacatta tacgaagtta tagatccagt actaatagga attgatttgg
     3241 atggtataaa cggaaacaaa aaaaagagct ggtactactt tctttaaaat tattttatta
     3301 tttgatttta tttaatagta tatattatat tttgaacgta gattattttg ttgaaagttg
     3361 ctgtagtgcc attgattcgt aacactaatt ctgtattagt cattcctctt gtttgatagt
     3421 atccaaaaaa acggctattt ttttgcaatc ttatttcctg catattatac agataacata
     3481 atgaaagaaa aaatcttttt ttttgttctt caatgatgat ttcaaccatt cttttaaaca
     3541 ttgatcaatt cctgagctca tcccattcat tccatcataa aatgtcgaca ctggatggcg
     3601 gcgttagtat cgaatcgaca gcagtatagc gaccagcatt cacatacgat tgacgcatga
     3661 tattactttc tgcgcactta acttcgcatc tgggcagatg atgtcgaggc gaaaaaaaat
     3721 ataaatcacg ctaacatttg attaaaatag aacaactaca atataaaaaa actatacaaa
     3781 tgacaagttc ttgaaaacaa gaatcttttt attgtcagta ctgactcgag ttattatgga
     3841 catggcatag acatatacaa agcttgttca ccatcggaag cagtaccatc gtataaagca
     3901 gtatccaaac cacacaaagt gaaacccatt cttctataag catgaatagc tggagcatta
     3961 acattggtaa cttccaacca caaatgacca gcacctcttt ctctggcgaa ttcagtagcc
     4021 aaacccatca aagctctacc aacaccatga cctctatgtt ctggagcaac ttcaatatct
     4081 tcaacagtca atcttctgtt ccaaccagaa taagaaacaa caacgaaacc agccaaatca
     4141 ccatcatcac cataagcaac gaaagttcta gaatctggat caccatcttc accagcatcg
     4201 gattcatcat cggattcatc atctgggaaa accttagtca atggtggatc aactggaact
     4261 tctctcaaag tgaaaccatc accagtagca gtaactctaa aaacagtatc ggtagtgaaa
     4321 gaaccatcca aagcttcaat agcttcagca tcacctggaa cagaagttct gtatctataa
     4381 gcagtatcat ccaaagtagt agacataatt gtaggatccg gttgtttatg ttcggatgtg
     4441 atgtgagaac tgtatcctag caagatttta aaaggaagta tatgaaagaa gaacctcagt
     4501 ggcaaatcct aaccttttat atttctctac aggggcgcgg cgtggggaca attcaacgcg
     4561 tctgtgaggg gagcgtttcc ctgctcgcag gtctgcagcg aggagccgta atttttgctt
     4621 cgcgccgtgc ggccatcaaa atgtatggat gcaaatgatt atacatgggg atgtatgggc
     4681 taaatgtacg ggcgacagtc acatcatgcc cctgagctgc gcacgtcaag actgtcaagg
     4741 agggtattct gggcctccat gtcgctggcc gggtgacccg gcggggacga ggcaagcttg
     4801 atggaagttc ctatactttc gctagcaatt gtctattcca agcctgtggc tttttttggt
     4861 gattcacaat acataaattg gcaaatacct cttctataga ctttaacctt tgatgaaatg
     4921 gtatggataa accacttttg tggtcagggt atatatattt ttattccaat ctggccttta
     4981 taaattcaaa tttccaaggg gactctgcta aaccttctga tataactata actctaataa
     5041 tggctgtaaa aagtgtgaaa aaaagatgat attcacaaaa aagataaagg ggatttcata
     5101 ttgggtatct agatttaaac atcgtatttc aacaatgaaa ctagaatcaa tacgaaatcc
     5161 agcgcacaac tgtgttcatt cccttgggaa ttatactgag taatgggaag aatgtgcaaa
     5221 aaatacccgt gtcttatata atttccattt gtgtagggct taaaagtgga caacttcagg
     5281 ttacgaaacc acgaaaatgt ctaaacgttt gatcaaattc gtaactcatg gaaaatcaca
     5341 gcaacaaatt aaggatggat atatccggta cttacaaata aaaacactaa agaagtataa
     5401 attttgaact gaaattctat agaaaatctt tttttttggg taaaattatg cgattgtggc
     5461 tcatagtaac gtaatcatac agttcaacaa acatcatttt gtcaaaggat ttctaatccc
     5521 attctttttc tacttggctt ttgagtatat atatatacat cctttgttca tcatcatgtt
     5581 cgattgaatt tttttcttgg ttgttattag attgtctact tgagtttttc ttggtgcaaa
     5641 ttgtggtgtt tttctaacaa atttttcaac atttaaagta tttctggaac ttttctacaa
     5701 ggaacgacat agatccatta atgcgcccct ctaaaacaat acccataatg tcttcttaag
     5761 tatgcatctg cttataaatg tttctggatt tactttagtt agaatgtcga cacgacaatg
     5821 aactattgct gtggatcacg tgcgatcact gaattatgag tttcttttta tttttctttt
     5881 tgggcttttt tttgcaattt tttttttcca cctttgctaa ttgcgtagtc acatataatg
     5941 tatacaattt ttgcaactct gacactataa ttgatccaat aataaagtat ataatagatc
     6001 atcaagtata cgtaatctcc ccaaaaaaaa attgcataaa ataaaatcta taaacctttt
     6061 ttagattttt agttgttcct tttttctttc tattcttcaa cttccaagtg ttgtcacttt
     6121 ctccctgtta actatattta aaagtaaata ccctcccccc cgggtatact cattaatcaa
     6181 taattatgtc taacttgttg accgttcacc aaaacttgcc agctttgcca gttgatgcta
     6241 cctctgatga agttagaaaa aacttgatgg atatgtttag agatagacaa gccttctctg
     6301 aacacacctg gaaaatgttg ttgtctgttt gtagatcctg ggctgcttgg tgtaaattga
     6361 acaacagaaa atggttccca gccgaaccag aagatgttag agattacttg ttgtacttgc
     6421 aagctagagg tttggctgtt aaaaccattc aacaacactt gggtcaattg aacatgttgc
     6481 acagaagatc cggtttgcca agaccatctg attctaacgc tgtttctttg gttatgagaa
     6541 gaattagaaa agaaaacgtt gatgctggtg aaagagctaa acaagccttg taggtataga
     6601 cactgaagaa aaaaaaattc tatcattgtt atgttgtgat ctttgatctt tagttgtcgg
     6661 gttaacacct gccaattgga tcaatacatc aatcaattaa ttctaatctt gaaaaaaaaa
     6721 ttcattttac taacaatttt ttcttttatt tagggctttc gaaagaaccg atttcgatca
     6781 agttagatcc ttgatggaaa actctgatag atgtcaagat attagaaact tggctttctt
     6841 gggtattgct tacaacacct tgttgagaat tgctgaaatt gctagaatta gagttaaaga
     6901 tatttccaga accgatggtg gtagaatgtt gattcacatt ggtagaacca aaaccttggt
     6961 ttctaccgct ggtgttgaaa aagccttgtc tttgggtgtt accaaattgg ttgaaagatg
     7021 gatttctgtt tctggtgttg ctgatgatcc aaacaactac ttgttctgta gagttagaaa
     7081 aaacggtgtt gctgctccat ctgctacctc tcaattatct accagagctt tggaaggtat
     7141 tttcgaagct acccacagat tgatttacgg tgctaaagat gattctggtc aaagatactt
     7201 ggcttggtct ggtcactctg ctagagttgg tgctgctaga gatatggcta gagccggcgt
     7261 ttctattcca gaaattatgc aagctggtgg ttggaccaac gttaacattg ttatgaacta
     7321 cattagaaac ttggattctg aaaccggtgc tatggttaga ttgttggaag atggtgatta
     7381 atttatacct aggtaagcaa atagctaaat tatatacgaa ttaatattat gattaagtgt
     7441 ttacgtgagt gcgatatttt tattactatc ttatacagtt gtatatactc tataaaatga
     7501 gttgtctatt aattaacgcg atgagtccct atttattttt ttatagttat gttagtatta
     7561 agaacgttat ttatatttca aattaattat ccatggataa cttcgtatag catacattat
     7621 acgaagttat tagggataac agggtaatct gcaggaattc gatatcaagc ttatcgatac
     7681 cgtcgacctc gagggggggc ccggtaccca gcttttgttc cctttagtga gggttaattc
     7741 cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     7801 ttccacacaa cataggagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     7861 ggtaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     7921 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7981 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8041 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8101 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8161 ttttccatag gctcggcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8221 ggcgaaaccc gacaggacta taaagatacc aggcgttccc ccctggaagc tccctcgtgc
     8281 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8341 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8401 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8461 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8521 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8581 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     8641 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8701 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8761 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8821 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8881 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8941 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctgcccgtcg
     9001 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9061 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9121 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9181 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     9241 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9301 caaggcgagt tacatgatcc cccatgttgt gaaaaaaagc ggttagctcc ttcggtcctc
     9361 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9421 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9481 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9541 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9601 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9661 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9721 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9781 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9841 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9901 aagtgccacc tgggtccttt tcatcacgtg ctataaaaat aattataatt taaatttttt
     9961 aatataaata tataaattaa aaatagaaag taaaaaaaga aattaaagaa aaaatagttt
    10021 ttgttttccg aagatgtaaa agactctagg gggatcgcca acaaatacta ccttttatct
    10081 tgctcttcct gctctcaggt attaatgccg aattgtttca tcttgtctgt gtagaagacc
    10141 acacacgaaa atcctgtgat tttacatttt acttatcgtt aatcgaatgt atatctattt
    10201 aatctgcttt tcttgtctaa taaatatata tgtaaagtac gctttttgtt gaaatttttt
    10261 aaacctttgt ttattttttt ttcttcattc cgtaactctt ctaccttctt tatttacttt
    10321 ctaaaatcca aatacaaaac ataaaaataa ataaacacag agtaaattcc caaattattc
    10381 catcattaaa agatacgagg cgcgtgtaag ttacaggcaa gcgatccgtc ctaagaaacc
    10441 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
