LOCUS       40924_28551        5956 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pLNHX_cHS4_650, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5956)
  AUTHORS   Suttiprapa S, Rinaldi G, Brindley PJ.
  TITLE     Prototypic chromatin insulator cHS4 protects retroviral transgene 
            from silencing in Schistosoma mansoni
  JOURNAL   Transgenic Res. 21 (3), 555-566 (2012)
  PUBMED    21918820
REFERENCE   2  (bases 1 to 5956)
  AUTHORS   Suttiprapa S, Rinaldi G, Brindley PJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2011) Microbiology, Immunology and Tropical 
            Medicine, George Washington University, 2300 Eye Street, NW, 
            Washington, DC 20037, USA
REFERENCE   3  (bases 1 to 5956)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5956)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Transgenic 
            Res."; date: "2012"; volume: "21"; issue: "3"; pages: "555-566"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-MAY-2011) Microbiology, Immunology and Tropical Medicine, George
            Washington University, 2300 Eye Street, NW, Washington, DC 20037, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             413..588
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    651..850
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1067..1858
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    2047..2052
                     /note="XhoI restriction binding site; Drosophila hsp70
                     promoter was removed from the parental pLNHX by XhoI enzyme
                     digestion"
     misc_feature    2203..2505
                     /label=3' Moloney murine leukemia virus LTR
                     /note="3' Moloney murine leukemia virus LTR"
     misc_feature    2500..2505
                     /label=XbaI restriction binding site
                     /note="XbaI restriction binding site"
     misc_feature    2506..3154
                     /note="minimal cHS4 insulator (cHS4 = chicken
                     hypersensitive site 4); Gallus gallus HS4 chromatin 
                     insulator, INSD U78775, was used as a template; comprises 
                     250 bp from the 5' active region of cHS4 fused to 400 bp of
                     the 3' region of cHS4; minimal insulator was chemically 
                     synthesized, sequence confirmed, and then inserted into the
                     XbaI site in U3 region of 3' LTR of pLNHX"
     misc_feature    3155..3451
                     /label=3' Moloney murine leukemia virus LTR
                     /note="3' Moloney murine leukemia virus LTR"
     misc_feature    3155..3160
                     /label=XbaI restriction binding site
                     /note="XbaI restriction binding site"
     misc_feature    3662..3802
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      3987
     rep_origin      complement(3988..4576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4750..5607)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5608..5712)
                     /label=AmpR promoter
     LTR             5956
                     /label=5' LTR
                     /note="long terminal repeat from Moloney murine sarcoma
                     virus"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcgggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt tctagtgaat
      301 catcagatgt ttccagggtg ccccaaggac ctgaaaatga ccctgtacct tatttgaact
      361 aaccaatcag ttcgcttctc gcttctgttc gcgcgcttcc gctctccgag ctcaataaaa
      421 gagcccacaa cccctcactc ggcgcgccag tcttccgata gactgcgtcg cccgggtacc
      481 cgtattccca ataaagcctc ttgctgtttg catccgaatc gtggtctcgc tgttccttgg
      541 gagggtctcc tctgagtgat tgactaccca cgacgggggt ctttcatttg ggggctcgtc
      601 cgggatttgg agacccctgc ccagggacca ccgacccacc accgggaggt aagctggcca
      661 gcaacttatc tgtgtctgtc cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt
      721 ctgtactagt tagctaacta gctctgtatc tggcggaccc gtggtggaac tgacgagttc
      781 tgaacacccg gccgcaaccc tgggagacgt cccagggact ttgggggccg tttttgtggc
      841 ccgacctgag gaagggagtc gatgtggaat ccgaccccgt caggatatgt ggttctggta
      901 ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttggaac
      961 cgaagccgcg cgtcttgtct gctgcagcca atagatccat ctcagcctag gattagttaa
     1021 agctgttttc cgatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     1081 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     1141 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     1201 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     1261 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     1321 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     1381 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     1441 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     1501 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     1561 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     1621 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     1681 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     1741 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     1801 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     1861 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     1921 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     1981 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccgggctc
     2041 gatcccctcg agatagatct atcgattagt ccaatttgtt aaagacagga tatcagtggt
     2101 ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata
     2161 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
     2221 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact
     2281 gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa
     2341 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc
     2401 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     2461 aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagaacggg gacagccccc
     2521 ccccaaagcc cccagggatg taattacgtc cctcccccgc tagggggcag cagcgagccg
     2581 cccggggctc cgctccggtc cggcgctccc cccgcatccc cgagccggca gcgtgcgggg
     2641 acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct
     2701 ctttgagcct gcagacacct ggggggatac ggggaaaaag ctttaggctt gtgtctgagc
     2761 ctgcatgttt gatggtgtct ggatgcaagc agaaggggtg gaagagcttg cctggagaga
     2821 tacagctggg tcagtaggac tgggacaggc agctggagaa ttgccatgta gatgttcata
     2881 caatcgtcaa atcatgaagg ctggaaaagc cctccaagat ccccaagacc aaccccaacc
     2941 cacccaccgt gcccactggc catgtccctc agtgccacat ccccacagtt cttcatcacc
     3001 tccagggacg gtgacccccc cacctccgtg ggcagctgtg ccactgcagc accgctcttt
     3061 ggagaaggta aatcttgcta aatccagccc gaccctcccc tggcacaacg taaggccatt
     3121 atctctcatc caactccagg acggagtcag tgagtctaga gaaccatcag atgtttccag
     3181 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     3241 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     3301 ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc
     3361 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
     3421 attgactacc cgtcagcggg ggtctttcat ttgggggctc gtccgggatc gggagacccc
     3481 tgcccaggga ccaccgaccc accaccggga ggtaagctgg ctgcctcgcg cgtttcggtg
     3541 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     3601 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     3661 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
     3721 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     3781 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3841 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3901 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3961 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4021 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4081 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4141 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4201 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4261 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4321 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4381 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     4441 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4501 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4621 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     4681 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4741 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4801 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4861 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4921 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4981 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     5041 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     5101 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     5161 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     5221 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     5281 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     5341 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     5401 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     5461 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     5521 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     5581 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5641 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5701 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     5761 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattcat
     5821 accagatcac cgaaaactgt cctccaaatg tgtccccctc acactcccaa attcgcgggc
     5881 ttctgcctct tagaccactc taccctattc cccacactca ccggagccaa agccgcggcc
     5941 cttccgtttc tttgct
//
