LOCUS       Exported                7714 bp DNA     circular SYN 20-JUL-2025
DEFINITION  Exported.
ACCESSION   V012423
VERSION     .
KEYWORDS    pLNCX2 ER:ras
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Young AR, Narita M, Ferreira M, Kirschner K, Sadaie M, Darot JF, 
            Tavare S, Arakawa S, Shimizu S, Watt FM, Narita M
  TITLE     Autophagy mediates the mitotic senescence transition.
  JOURNAL   Genes Dev. 2009 Apr 1;23(7):798-803. doi: 10.1101/gad.519709. Epub 
            2009 Mar 11.
  PUBMED    19279323
REFERENCE   2  (bases 1 to 7714)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7714)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7714)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.519709"; journalName: "Genes Dev"; date: "2009-04-1-
            1"; volume: "23"; issue: "7"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..589
                     /label=5' LTR
                     /note="long terminal repeat from Moloney murine sarcoma
                     virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     CDS             1512..2303
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     enhancer        2377..2680
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2681..2884
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             3336..3698
                     /gene="ESR1"
                     /label=Estrogen receptor
                     /note="Estrogen receptor from Macaca mulatta. Accession#: 
                     P49886"
     CDS             3936..4502
                     /label=H-Ras (G12V)
                     /note="human oncoprotein generated by the G12V mutation in
                     the small GTPase H-Ras"
     LTR             4616..5209
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     primer_bind     complement(5312..5334)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5420..5560
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5575..5592)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5746..6334)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6508..7365)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7366..7470)
                     /label=AmpR promoter
     primer_bind     7538..7556
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aattccgatc tgatcaagag acaggatgag
     1501 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     1561 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     1621 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     1681 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     1741 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     1801 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     1861 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     1921 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     1981 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     2041 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     2101 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     2161 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     2221 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     2281 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaatagt aatcaattac
     2341 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     2401 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     2461 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     2521 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2581 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2641 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     2701 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     2761 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     2821 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     2881 agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag
     2941 cttgttgatc caccatgcga aatgaaatgg gtgcttcagg agacatgagg gctgccaacc
     3001 tttggccaag ccctcttgtg attaagcaca ctaagaagaa tagccctgcc ttgtccttga
     3061 cagctgacca gatggtcagt gccttgttgg atgctgaacc gcccatgatc tattctgaat
     3121 atgatccttc tagacccttc agtgaagcct caatgatggg cttattgacc aacctagcag
     3181 atagggagct ggttcatatg atcaactggg caaagagagt gccaggcttt ggggacttga
     3241 atctccatga tcaggtccac cttctcgagt gtgcctggct ggagattctg atgattggtc
     3301 tcgtctggcg ctccatggaa cacccgggga agctcctgtt tgctcctaac ttgctcctgg
     3361 acaggaatca aggtaaatgt gtggaaggca tggtggagat ctttgacatg ttgcttgcta
     3421 cgtcaagtcg gttccgcatg atgaacctgc agggtgaaga gtttgtgtgc ctcaaatcca
     3481 tcattttgct taattccgga gtgtacacgt ttctgtccag caccttgaag tctctggaag
     3541 agaaggacca catccaccgt gtcctggaca agatcacaga cactttgatc cacctgatgg
     3601 ccaaagctgg cctgactctg cagcagcagc atcgccgcct agctcagctc cttctcattc
     3661 tttcccatat ccggcatatg agtaacaaac gcatggagca tctctacaac atgaaatgca
     3721 agaacgtggt acccctctat gacctgctcc tggagatgtt ggatgcccac cgccttcatg
     3781 ccccagccag tcgcatggga gtgcccccag aggagcccag ccagacccag ctggccacca
     3841 ccagctccac ttcagcacat tccttacaaa cctactacat acccccggaa gcagagggct
     3901 tccccaacga tatcccacgt ggaggcctga attccatgac ggaatataag ctggtggtgg
     3961 tgggcgccgt cggtgtgggc aagagtgcgc tgaccatcca gctgatccag aaccattttg
     4021 tggacgaata cgaccccact atagaggatt cctaccggaa gcaggtggtc attgatgggg
     4081 agacgtgcct gttggacatc ctggataccg ccggccagga ggagtacagc gccatgcggg
     4141 accagtacat gcgcaccggg gagggcttcc tgtgtgtgtt tgccatcaac aacaccaagt
     4201 cttttgagga catccaccag tacagggagc agatcaaacg ggtgaaggac tcggatgacg
     4261 tgcccatggt gctggtgggg aacaagtgtg acctggctgc acgcactgtg gaatctcggc
     4321 aggctcagga cctcgcccga agctacggca tcccctacat cgagacctcg gccaagaccc
     4381 ggcagggagt ggaggatgcc ttctacacgt tggtgcgtga gatccggcag cacaagctgc
     4441 ggaagctgaa ccctcctgat gagagtggcc ccggctgcat gagctgcaag tgtgtgctct
     4501 cctgacgcaa ttcgatatca agcttgtttg gccgaggcgg ccgcttgtcg acaggcctta
     4561 atggcctaac atcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     4621 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     4681 aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg
     4741 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     4801 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     4861 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     4921 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     4981 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     5041 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     5101 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     5161 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt
     5221 ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg taagctggct
     5281 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     5341 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     5401 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     5461 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     5521 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     5581 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     5641 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     5701 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     5761 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     5821 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     5881 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5941 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6001 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6061 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6121 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6181 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6241 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6301 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6361 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6421 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     6481 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     6541 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6601 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6661 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6721 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6781 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     6841 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6901 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6961 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7021 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7081 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     7141 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7201 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7261 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7321 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     7381 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7441 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     7501 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     7561 tcgtcttcaa gaattcatac cagatcaccg aaaactgtcc tccaaatgtg tccccctcac
     7621 actcccaaat tcgcgggctt ctgcctctta gaccactcta ccctattccc cacactcacc
     7681 ggagccaaag ccgcggccct tccgtttctt tgct
//
