LOCUS       40924_28521        8416 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLN-ENR-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Nkrumah LJ, Muhle RA, Moura PA, Ghosh P, Hatfull GF, Jacobs WR Jr., 
            Fidock DA.
  TITLE     Efficient site-specific integration in Plasmodium falciparum 
            chromosomes mediated by mycobacteriophage Bxb1 integrase
  JOURNAL   Nat. Methods 3 (8), 615-621 (2006)
  PUBMED    16862136
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2006) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   3  (bases 1 to 8416)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2007) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   4  (bases 1 to 8416)
  AUTHORS   Nkrumah LJ, Fidock DA.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2009) Microbiology and Immunology, Albert Einstein
            College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA
REFERENCE   5  (bases 1 to 8416)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 8416)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2006"; volume: "3"; issue: "8"; pages: "615-621"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUN-2006) Microbiology and Immunology, Albert Einstein College 
            of Medicine, 1300 Morris Park, Bronx, NY 10461, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (17-JAN-2007) Microbiology and Immunology, Albert Einstein College 
            of Medicine, 1300 Morris Park, Bronx, NY 10461, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "Submitted 
            (10-JUL-2009) Microbiology and Immunology, Albert Einstein College 
            of Medicine, 1300 Morris Park, Bronx, NY 10461, USA"
COMMENT     SGRef: number: 5; type: "Journal Article"
COMMENT     On Jul 10, 2009 this sequence version replaced DQ813653.2.
FEATURES             Location/Qualifiers
     source          1..8416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(40..876)
                     /label=derived from hsp86 3' UTR
                     /note="derived from hsp86 3' UTR"
                     /regulatory_class="terminator"
     CDS             complement(885..1595)
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     misc_feature    complement(1602..2897)
                     /gene="enr-gfp"
                     /label=ENR
                     /note="ENR"
     misc_feature    complement(2905..3916)
                     /label=contains promoter and 5' UTR from calmodulin gene
                     /note="contains promoter and 5' UTR from calmodulin gene"
     misc_feature    3999..4593
                     /label=contains promoter and 5' UTR from PcDT gene
                     /note="contains promoter and 5' UTR from PcDT gene"
     CDS             4611..5003
                     /label=BSD
                     /note="blasticidin S deaminase"
     regulatory      5012..5608
                     /label=derived from hrp2 3' UTR
                     /note="derived from hrp2 3' UTR"
                     /regulatory_class="terminator"
     misc_feature    complement(5622..5728)
                     /label=tandem attP elements
                     /note="tandem attP elements"
     misc_recomb     complement(5622..5672)
                     /label=attP
                     /note="attP"
     misc_recomb     complement(5679..5728)
                     /label=attP
                     /note="attP"
     promoter        complement(5734..5751)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5758..5774)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6248..6352
                     /label=AmpR promoter
     CDS             6353..7210
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7384..7972
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8260..8281
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8296..8326
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8334..8350
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8358..8374
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8392..8410
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 aagcttgggg ggatcctcta gagtcgacct gcaggcatgc tatttgatga attaactaca
       61 cttaaaataa tacaattatt attaaatttt tttttgattt atttattaat ttttaaactt
      121 aatcatttgt atttgggagg aattatatat atctttataa ttattttatt tttttttatt
      181 tttttatttt tttattatta ttattttttt ttattttttt tttttactgt atcaaagaaa
      241 aacctttaaa aaaaaaatta taatttcccc atcttactat atttttaata catacgtttt
      301 aaggaattaa attagacaaa agctatatta tgctttacat ataattagaa tttataaacg
      361 tttggttatt agatatttca tgtctcagta aagtctttca atacatatgt aaaaaaatat
      421 atatgaatac acataagttg ttaatatatt ttatatgcat aaatgtataa atatatatat
      481 atatatatat atatgtatgt atgtatatgt gtgtatatga aattatttca atgtttaatt
      541 ttttaaattt taattttttt tttttttttt ttttttatta tgtatattga tctttattat
      601 ttaaatatta cttttttcgt tttttcttct ttttattatt tttttttttt tttatatttt
      661 atacaaatgg taattcaaat aaaaggtata aatttatatt taattttctt ttatggataa
      721 ataaaagaaa aatataaata tataaaaata taaaaatata tatatgtata ttggggtgat
      781 gataaaatga aagataatat atatatatat atatctttat tttttttttt ttgtagaccc
      841 cattgtgagt acataaatat attatataac tcgaccttaa gttatttgta tagttcatcc
      901 atgccatgtg taatcccagc agctgttaca aactcaagaa ggaccatgtg gtctctcttt
      961 tcgttgggat ctttcgaaag ggcagattgt gtggacaggt aatggttgtc tggtaaaagg
     1021 acagggccat cgccaattgg agtattttgt tgataatggt ctgctagttg aacgcttcca
     1081 tcttcaatgt tgtgtctaat tttgaagtta actttgattc cattcttttg tttgtctgcc
     1141 atgatgtata cattgtgtga gttatagttg tattccaatt tgtgtccaag aatgtttcca
     1201 tcttctttaa aatcaatacc ttttaactcg attctattaa caagggtatc accttcaaac
     1261 ttgacttcag cacgtgtctt gtagttcccg tcatctttga aaaatatagt tctttcctgt
     1321 acataacctt cgggcatggc actcttgaaa aagtcatgct gtttcatatg atctgggtat
     1381 ctcgcaaagc attgaagacc atacgcgaaa gtagtgacaa gtgttggcca tggaacaggt
     1441 agttttccag tagtgcaaat aaatttaagg gtaagttttc cgtatgttgc atcaccttca
     1501 ccctctccac tgacagaaaa tttgtgccca ttaacatcac catctaattc aacaagaatt
     1561 gggacaactc cagtgaaaag ttcttctcct ttactcgtac gttcattttc attgcgatat
     1621 atatcatctg gtaaaaacat tatatttaat ccgttatcca catatattgt ctgcccagtt
     1681 atcgcacgac tctctcgaga taacaaaaat gatgctacag atccaatatc tgttgataat
     1741 aacttttgac gtaatggagc atacttttct gaatactcta ttgcataatc tataaatgta
     1801 taattttggc tagctgaatt cttcttctct tccttctcac ctgaattgtt cataatatta
     1861 tgaacatcat gactattcct atttttattt tgatttgtat tattttcata tgtattgttt
     1921 aatttattaa tagcagtagc tgctcttgat ttaagtggtc ctgcactgat agtattaatt
     1981 ctgatattat aatttcttcc tagatgataa gctaaaactc tggtatcaga ttcgagtgca
     2041 gctttagcgc tggacatacc tccaccatag cctggcacaa ctttttggct agcatgatat
     2101 gttaatgaaa taatgctgga ttgaggtttc ataatattga caaaatattt acataatgat
     2161 attaaagagt aagaactttt actaagagca tctaaataac cttttcgact ggtatttaat
     2221 aaatcttttt gaacttcttt agcgttagct aaagaatgaa cgagcatatt aatttttcca
     2281 tatttttgat gtattaaatt ggcaacatct tctgtagtat aattctgtaa catattatat
     2341 cttttattat ttttcgtttc ttcatctata tcattggcag tatcaaaaga agcgtcaaag
     2401 ggtagcatgt ctaatatatt cattttctta tctttatcaa taatcatatc attatcaaat
     2461 ttcccgtttt tataattttt cataaaaata ttataaacag gaggccaaat accgaaaatt
     2521 atttttacat ttcttttgga taattcttta gcaatacccc acccgtatcc atttgtatct
     2581 cctataccag caataaaaca aatatcttca tttttatttt cttctttaat tttgtttatg
     2641 tttgatgcat cttgttcttc tttctccata taatgattat tattgtgtgt attattcata
     2701 ttatttaagt gtttcatttt gtttcctata aatatgtttt cgcgtttttc tttcctataa
     2761 aatgcttttt ccttacctcg tatctgttca ttctgaagaa cgttatggaa tgtcttttga
     2821 gtattattct ggataaagca cacaatggta taaaaatgta gaaagaggaa taataaccgt
     2881 tgtgatattt tatccattta tcacctaggt gatatatttc tattaggtat ttattattat
     2941 aaaatataaa tcttgaatga taataaataa aatattagtt attccttttc tagtttaaaa
     3001 tatacatatt ataaatatat atatatatat atatattttt attgtgacaa gaatatataa
     3061 ttataaatta tattatttat ttttgtattt tttttttttt tttttttttt ttcttttttt
     3121 gttttatttt tctttttttt tataaatatt atttttttct tttatcatgc acattggaat
     3181 aatacattaa tatatatata tatattatat tatacatata ttgaataatg tttataaaaa
     3241 atgcataact tatatgaata taattttttt taaatatgac aaaaagaaaa aaaaaaaaaa
     3301 ccaaaaaaaa ttaaaattga aatgaaatat ataaatatat tatttatata tattatacat
     3361 tgtttaatac tactacatgt atatatatat attatatata tatatatata tcaatttttt
     3421 caaaaataaa ttaatataaa aagaggggaa aaaaaaaaaa aaaaaaaaaa aaagataatt
     3481 aagtaagcat ttaaaaatat ataaattgat aatatataaa attaatcaca tataaactaa
     3541 tataatttat aaaataagga aaataaaata ttaccataaa ataaaaataa aaataaaaaa
     3601 aaaaaaaaaa aacacctttt tttatatata ttaatatata attatctctt agaaaaaata
     3661 ttgtataatt atatatgtaa tgatttatat aaaaaaataa aattatacaa gtatatattt
     3721 tgtttctata aattgatatc ttaattattt attattagaa atagatattt ttataataaa
     3781 ccaatagata aaatttgtag agaaaaaaaa ataaaaataa aaataaaaat aatataatat
     3841 ataataaaat aaaataatat tatataaata tattttaatt ttttttacaa aatggttaac
     3901 aaagaagaag ctcagaggca tgcaagcttc gatccatata attattaata ggtacttttt
     3961 ttttttatat atgggaattt ccttataggg cccgcatgct tagctaattc gcttgtaaga
     4021 ggtactctcg tttatgcaaa actatttgat atagcatttt aacaagtaca catatatata
     4081 tgtaatatat atactatata tatctattgc atgtgtacta agcatgtgca tggcatcccc
     4141 tttttctcgt gtttaaaaca gtttgtatga taaaatataa aggatttgaa aaagagaaaa
     4201 aaatatatga tctcatccta tatagcgcca taatttttat ttgggttgaa taaaattttc
     4261 tactaaattt aggtgtaagt aaaataatgg aatatatata agtacaataa aaaagtgcat
     4321 aaattaaaaa atttttataa taaatatttt ttttaaaaaa gtcaataata atattaaata
     4381 tatataacac aggattatat atgttcacta caatttttta tattataata taaattcttt
     4441 tcaattttca ttttatttta catacacttt ccttttttgt cactatattt taatattcac
     4501 atatttagtt taaatactgg ctatttcttt ctacatttgc tagtaacaat tgtgtagtgc
     4561 ttatatatat acacacacct aaaacttaca aaccatggtg ataaatgcat gccaagcctt
     4621 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca
     4681 tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg
     4741 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg
     4801 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg
     4861 gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca
     4921 aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc
     4981 cctctggtta tgtgtgggag ggctaaggta ccccattaaa tttatttaat aatagattaa
     5041 aaatattata aaaataaaaa cataaacaca gaaattacaa aaaaaataca tatgaatttt
     5101 ttttttgtaa tcttccttat aaatatagaa taatgaatca tataaaacat atcatttttc
     5161 atttatttac atttaaaatt attgtttcag tatctttaat ttattatgta tatataaaaa
     5221 taacttacaa ttttattaat aaacaatata tgtttattaa ttcatgtttt gtaatttatg
     5281 ggatagcgat tttttttact gtctgtattt ttctttttta attatgtttt aattgtattt
     5341 tatttttatt attgttcttt ttatagtatt attttaaaac aaaatgtttt ttctaagaac
     5401 ttataataat aataaatata aattttaata aaaattatat ttatctttta caatatgaac
     5461 ataaagtaca acattaatat atagctttta atatttttat tcctaatcat gtaaatctta
     5521 aatttttctt tttaaacata tgttaaatat ttatttctca ttatatataa ggacatattt
     5581 attaaaccgc agagaaatct agaggtaccg agctcgaatt ccgggtttgt accgtacacc
     5641 actgagaccg cggtggttga ccagacaaac cagaattcgg gtttgtaccg tacaccactg
     5701 agaccgcggt ggttgaccag acaaaccaga attctatagt gagtcgtatt acaattcact
     5761 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     5821 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     5881 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
     5941 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     6001 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     6061 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     6121 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     6181 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     6241 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6301 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     6361 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     6421 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     6481 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     6541 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     6601 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     6661 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     6721 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     6781 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     6841 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     6901 aatgccaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     6961 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     7021 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     7081 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     7141 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     7201 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     7261 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     7321 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     7381 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     7441 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     7501 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     7561 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     7621 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     7681 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     7741 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     7801 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     7861 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     7921 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatcga aaaacgccag
     7981 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     8041 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     8101 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     8161 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     8221 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     8281 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     8341 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tatttaggtg
     8401 acactataga atactc
//
