LOCUS       V004969                 6862 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004969
VERSION     V004969
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Liu M, Wang M.
  TITLE     Development of a plasmid-based methods for expression and antisense
            gene knockdown in Riemerella anatipestifer and application
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Liu M, Wang M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2016) Institute of Preventive Veterinary Medicine,
            Sichuan Agricultural University, No. 211, Huimin Road, Chengdu,
            Sichuan 611130, China
REFERENCE   3  (bases 1 to 6862)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6862)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-MAR-2016) Institute of Preventive Veterinary Medicine, Sichuan
            Agricultural University, No. 211, Huimin Road, Chengdu, Sichuan
            611130, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    290..311
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        326..356
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    364..380
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     388..404
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2819..2836
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(3508..4470)
                     /gene="cfxA"
                     /label="Beta-lactamase"
                     /note="Beta-lactamase from Phocaeicola vulgatus.
                     Accession#: P30899"
     primer_bind     complement(4650..4666)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5140..5244
                     /label="AmpR promoter"
     CDS             5245..6102
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6276..6862
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
       61 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      121 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      181 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      241 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      301 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      361 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      421 ttgggctatt taggcattag ccctcttttt aacctgactt aaaaaataat taaatcataa
      481 gatattaaat atcaagtgat tatgatttac tatgctatac ataatatcac ccagtgttag
      541 aaaattcttg ctatgctttt tgtttgtttt ttttcattcg tgaactttta gaatactttt
      601 aaaataaact caaatttata aactattgat tttcaatatt atacaaaacc aaacttatta
      661 acattaaact tatttctatt acctttaaaa taaactacta aaagacaaat ctaccaatcc
      721 tcggaactat ttaataaata ggaagttata taaaaataat gaacttttag aatactttat
      781 tagtcttttt agctccaaaa atgaactttt agaatacttt ttttgaactt ttagaatact
      841 tttttaaact tgctttttag taagttcatt ttttatatat ttgtattcta aaagtgcatt
      901 ttatattcat aactctctat atatggcaaa agtagataat aaactaatgt ttcagtccta
      961 tatttttact acggctaaat atgatttttc agtctatgaa aagcgtatac tatataggca
     1021 aatagaaata gaacaggcat tgctaaataa tgaggcgttg aaagattgta tcaaaataga
     1081 tactaaccta tggggggata aaaaatatac aattccaatt tctatgctgt tagctaatga
     1141 tgaagataaa aattattata aaattaaaaa agcattcaaa gacctaaaat caaagaacat
     1201 tgagtacgaa gatgaaaaag aatacgcttg ctttggtgtt attgataagt tttggattga
     1261 taaatatgga agaaatgtta cttggcaatc tgacaaaaga attgttgaag cggtaatgga
     1321 ttttacaaga ggatggagaa aatatgaatt gaagattgct atgcagtttg agactattta
     1381 tgcaatgcgt ttttacgaac tgatagctaa taaaaccaaa ccaataacat atgaagtgtc
     1441 atttcttgaa aaaatgtttc aattggaaaa taagtacagg aaccctcagg gtaatcttaa
     1501 tatcagtatg tttaaacgcc gtgtattaga ccccgctaaa aacgagttag ataaatgttc
     1561 tccatacact tttgattata ctctttcaaa ggataaaaaa attatttctc taattcctat
     1621 tttccaagaa caatttgcag atgaaagtat gaagatgatc aaaatagaag aggaaaaaga
     1681 tatacactct gtattaactc aaagagaaat tgatgttttt acaaacgaat ttggttttac
     1741 acaacaaggt ttattaaaca actatgccct attcaaagat tgtaaggcta cacttccatc
     1801 aaactattct ttcttccttt ttcaagagat aagaaaaagt cggactaaga aaggaaaaat
     1861 atctcctgct tatgtgattg ggattataag aaatatgctt aaagatttca aaaaagaaca
     1921 acaataggct tatgaaaaaa tatacaacaa aagaaatcgc agagcttttt ggcgtcagcg
     1981 aacggacaat ccagcgacac ataacgacac taagcgacaa catatcaaat accgacagaa
     2041 agggcttttc aataccagaa gacattgttt tctttcttgc cgagcgacac cattacgaca
     2101 ttttagcgac aacgaacgac aacgaacgac aggataaaaa tgaagaattt cctcatattg
     2161 aatacttcac agaagaggaa taccaagagt ttaaaaaaag gatcacagag tacccttttt
     2221 tgaaagagca aattaaactg tctaatgaac atttggatac tctaaaaaca caaatagaat
     2281 atttcagact atcttacaat aagcaattgg atattcacga aaaattaatc ggagcgttcc
     2341 gagaaagaaa ctttattgaa gctaaagaaa aggggatgga taaatagctc ctaataatta
     2401 aagaagtatt agaaaaaaaa tgctatataa aaacttaatc gagctctata cgagatctgt
     2461 tccaatgcat tggctgcagg tcgacaaatc accaatgatt ggtactttct acagaaaacc
     2521 atcgccagat aaagatgttt ttgtaaatgt aggagattct gtatctgagg ggaaggtagt
     2581 ttgcgttata gaagctatga aactatttaa ccaaatagag agtgaggtaa gtggtaagat
     2641 agttaaaata ttggtagatg atgcaactcc tgtggagtat gatcagccat tattccttgt
     2701 tgatccgtct taagtagaga aattttaagt caaaaaagtg ttacagtttg ctctgttgat
     2761 atggggtaat ttatttttaa tttaaagttt cttataaacc atggctctag aactcgagca
     2821 tcatcatcat catcattgaa ctagtggagt tgcttctctt ttcgggagtg gaaaactgaa
     2881 agaactggaa cgtgccaacg aaaaactgca agacgaggtt tcaaaacgga acaccaatat
     2941 tgaaaaattg cagagccaag tacagcagat gcagaaacag catgatacgc aaatccacaa
     3001 tctcagagaa atgcacaggc aggaacttga catgaaagaa aaagaactgt cacggctcgc
     3061 cagaatcata gacaaggctt tcaggtggtt tccgatgttc agggaaatgc tgcgcatgga
     3121 aaagttttgt gccatgctgg gattctctaa agaaatgact gaaagtctta tagtcaaaaa
     3181 agaagccctg aaatgtagcg gtaaaatcta ttccgagcaa cacaggcgga actttgatat
     3241 aaaggatgat attttaaggg tggaaaatga ccctgacgat gaaagcaggc tgaacctgac
     3301 aataaacagg aagccgattg ccgactggtt cagggagcaa tggcacaggc ttagatatgg
     3361 agcaagagtg ccgcaacagg aagaaagaaa aagtagagga ttcaaattat aatagaagca
     3421 atttgattag taatctaaaa gcactccgat aacgattaga gtgcttttag attgtttatc
     3481 attaattatc aaagcaagtg cagtttaaga ttttactgaa gtttgcatta ataaagaata
     3541 tactacagct gatatatgcg caacatattg tgacgcttgt gatttatttc ccttgaaatc
     3601 cttaacaaat accgctaagg tataactgat attattaggc agacatatat aggcaacatc
     3661 attgtgagct gcaagaacac cattttcatt aacataacct gaacctgtct tatgcgctat
     3721 aacaacccct tctttatcaa gaagtggagc tgctatccta tctacacctg ttttgcattc
     3781 ttttaacgta ttcttaatga aactttgttt ctcatcatcg ataagacctt cagtaaacaa
     3841 acgattcatc aacattgcag caccaagagg agatgtatag ttagagtaag ccttgttatg
     3901 gtcagccgac atttcctctt ccgtataagc tatctgaaaa cttgaacgag gaatgagtgt
     3961 ggctataaaa ctatctgttt gagcgacatt aaccatatcc ttaaacataa ggttgcttgc
     4021 attgttgtca ctctgagtaa gagtataacg cagcaaatct ctcactgtca atgatatgac
     4081 tggccctgaa taatctttca gcataggact ccaagtcttt gggtcaagtt tatccctatt
     4141 tatatttact aaggtatcaa gtgaaattcc tttattgtca aagtcattac aaagagctaa
     4201 tgcctgatga accttaaaca cactcatcat aggataaaca ctcttattat tgaccttaac
     4261 cgtatctctg ttattaacaa taaccgccac accaatttcg ccaggacaag ctgagacaat
     4321 ttgagaaatg ctatcagtca aaacatttgt taaaggagga tttgcgctat cttttgtcgc
     4381 tgatttatgg aacaatgaaa ataccaagat gaaaatgcaa actaaagcta tactcaaaac
     4441 tacgatttgt ttttttctgt ttttttccat gttttatatt atttatattt gtttgacgag
     4501 aatatcttta tttgccgaca aaggtacata actaaagttt cccacccaaa taaatagata
     4561 gaaaaataac agtttgtcga attttctttg taaattagta atcgctaaag aactgatttt
     4621 ggggatcccc gggtaccgag ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg
     4681 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     4741 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     4801 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     4861 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     4921 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     4981 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     5041 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5101 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5161 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5221 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5281 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5341 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5401 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5461 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5521 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5581 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5641 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5701 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5761 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5821 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5881 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5941 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     6001 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6061 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6121 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6181 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6241 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6301 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6361 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6421 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6481 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6541 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6601 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6661 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6721 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6781 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6841 tcaggggggc ggagcctatg ga
//
