LOCUS       V004971                 6733 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004971
VERSION     V004971
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Liu M, Wang M.
  TITLE     Development of a plasmid-based methods for expression and antisense
            gene knockdown in Riemerella anatipestifer and application
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6733)
  AUTHORS   Liu M, Wang M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2016) Institute of Preventive Veterinary Medicine,
            Sichuan Agricultural University, No. 211, Huimin Road, Chengdu,
            Sichuan 611130, China
REFERENCE   3  (bases 1 to 6733)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6733)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-MAR-2016) Institute of Preventive Veterinary Medicine, Sichuan
            Agricultural University, No. 211, Huimin Road, Chengdu, Sichuan
            611130, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    290..311
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        326..356
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    364..380
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     388..404
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2690..2707
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(3379..4341)
                     /gene="cfxA"
                     /label="Beta-lactamase"
                     /note="Beta-lactamase from Phocaeicola vulgatus.
                     Accession#: P30899"
     primer_bind     complement(4521..4537)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5011..5115
                     /label="AmpR promoter"
     CDS             5116..5973
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6147..6733
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
       61 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      121 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      181 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      241 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      301 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      361 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      421 ttgggctatt taggcattag ccctcttttt aacctgactt aaaaaataat taaatcataa
      481 gatattaaat atcaagtgat tatgatttac tatgctatac ataatatcac ccagtgttag
      541 aaaattcttg ctatgctttt tgtttgtttt ttttcattcg tgaactttta gaatactttt
      601 aaaataaact caaatttata aactattgat tttcaatatt atacaaaacc aaacttatta
      661 acattaaact tatttctatt acctttaaaa taaactacta aaagacaaat ctaccaatcc
      721 tcggaactat ttaataaata ggaagttata taaaaataat gaacttttag aatactttat
      781 tagtcttttt agctccaaaa atgaactttt agaatacttt ttttgaactt ttagaatact
      841 tttttaaact tgctttttag taagttcatt ttttatatat ttgtattcta aaagtgcatt
      901 ttatattcat aactctctat atatggcaaa agtagataat aaactaatgt ttcagtccta
      961 tatttttact acggctaaat atgatttttc agtctatgaa aagcgtatac tatataggca
     1021 aatagaaata gaacaggcat tgctaaataa tgaggcgttg aaagattgta tcaaaataga
     1081 tactaaccta tggggggata aaaaatatac aattccaatt tctatgctgt tagctaatga
     1141 tgaagataaa aattattata aaattaaaaa agcattcaaa gacctaaaat caaagaacat
     1201 tgagtacgaa gatgaaaaag aatacgcttg ctttggtgtt attgataagt tttggattga
     1261 taaatatgga agaaatgtta cttggcaatc tgacaaaaga attgttgaag cggtaatgga
     1321 ttttacaaga ggatggagaa aatatgaatt gaagattgct atgcagtttg agactattta
     1381 tgcaatgcgt ttttacgaac tgatagctaa taaaaccaaa ccaataacat atgaagtgtc
     1441 atttcttgaa aaaatgtttc aattggaaaa taagtacagg aaccctcagg gtaatcttaa
     1501 tatcagtatg tttaaacgcc gtgtattaga ccccgctaaa aacgagttag ataaatgttc
     1561 tccatacact tttgattata ctctttcaaa ggataaaaaa attatttctc taattcctat
     1621 tttccaagaa caatttgcag atgaaagtat gaagatgatc aaaatagaag aggaaaaaga
     1681 tatacactct gtattaactc aaagagaaat tgatgttttt acaaacgaat ttggttttac
     1741 acaacaaggt ttattaaaca actatgccct attcaaagat tgtaaggcta cacttccatc
     1801 aaactattct ttcttccttt ttcaagagat aagaaaaagt cggactaaga aaggaaaaat
     1861 atctcctgct tatgtgattg ggattataag aaatatgctt aaagatttca aaaaagaaca
     1921 acaataggct tatgaaaaaa tatacaacaa aagaaatcgc agagcttttt ggcgtcagcg
     1981 aacggacaat ccagcgacac ataacgacac taagcgacaa catatcaaat accgacagaa
     2041 agggcttttc aataccagaa gacattgttt tctttcttgc cgagcgacac cattacgaca
     2101 ttttagcgac aacgaacgac aacgaacgac aggataaaaa tgaagaattt cctcatattg
     2161 aatacttcac agaagaggaa taccaagagt ttaaaaaaag gatcacagag tacccttttt
     2221 tgaaagagca aattaaactg tctaatgaac atttggatac tctaaaaaca caaatagaat
     2281 atttcagact atcttacaat aagcaattgg atattcacga aaaattaatc ggagcgttcc
     2341 gagaaagaaa ctttattgaa gctaaagaaa aggggatgga taaatagctc ctaataatta
     2401 aagaagtatt agaaaaaaaa tgctatataa aaacttaatc gagctctata cgagatctgt
     2461 tccaatgcat tggctgcagg tcgacatttc aaaaatttaa cttaaaccac tgatttacaa
     2521 aaatgtttat ttgcaaaact attttacaaa gatattacaa taatttttct agcacaaatt
     2581 tatttctcac catatctttg ccttgttcaa gtgagtgaac agagtaacaa acaaaaaaag
     2641 aaacaaattt aaaaaaatta tttaaaattc catggctcta gaactcgagc atcatcatca
     2701 tcatcattga actagtggag ttgcttctct tttcgggagt ggaaaactga aagaactgga
     2761 acgtgccaac gaaaaactgc aagacgaggt ttcaaaacgg aacaccaata ttgaaaaatt
     2821 gcagagccaa gtacagcaga tgcagaaaca gcatgatacg caaatccaca atctcagaga
     2881 aatgcacagg caggaacttg acatgaaaga aaaagaactg tcacggctcg ccagaatcat
     2941 agacaaggct ttcaggtggt ttccgatgtt cagggaaatg ctgcgcatgg aaaagttttg
     3001 tgccatgctg ggattctcta aagaaatgac tgaaagtctt atagtcaaaa aagaagccct
     3061 gaaatgtagc ggtaaaatct attccgagca acacaggcgg aactttgata taaaggatga
     3121 tattttaagg gtggaaaatg accctgacga tgaaagcagg ctgaacctga caataaacag
     3181 gaagccgatt gccgactggt tcagggagca atggcacagg cttagatatg gagcaagagt
     3241 gccgcaacag gaagaaagaa aaagtagagg attcaaatta taatagaagc aatttgatta
     3301 gtaatctaaa agcactccga taacgattag agtgctttta gattgtttat cattaattat
     3361 caaagcaagt gcagtttaag attttactga agtttgcatt aataaagaat atactacagc
     3421 tgatatatgc gcaacatatt gtgacgcttg tgatttattt cccttgaaat ccttaacaaa
     3481 taccgctaag gtataactga tattattagg cagacatata taggcaacat cattgtgagc
     3541 tgcaagaaca ccattttcat taacataacc tgaacctgtc ttatgcgcta taacaacccc
     3601 ttctttatca agaagtggag ctgctatcct atctacacct gttttgcatt cttttaacgt
     3661 attcttaatg aaactttgtt tctcatcatc gataagacct tcagtaaaca aacgattcat
     3721 caacattgca gcaccaagag gagatgtata gttagagtaa gccttgttat ggtcagccga
     3781 catttcctct tccgtataag ctatctgaaa acttgaacga ggaatgagtg tggctataaa
     3841 actatctgtt tgagcgacat taaccatatc cttaaacata aggttgcttg cattgttgtc
     3901 actctgagta agagtataac gcagcaaatc tctcactgtc aatgatatga ctggccctga
     3961 ataatctttc agcataggac tccaagtctt tgggtcaagt ttatccctat ttatatttac
     4021 taaggtatca agtgaaattc ctttattgtc aaagtcatta caaagagcta atgcctgatg
     4081 aaccttaaac acactcatca taggataaac actcttatta ttgaccttaa ccgtatctct
     4141 gttattaaca ataaccgcca caccaatttc gccaggacaa gctgagacaa tttgagaaat
     4201 gctatcagtc aaaacatttg ttaaaggagg atttgcgcta tcttttgtcg ctgatttatg
     4261 gaacaatgaa aataccaaga tgaaaatgca aactaaagct atactcaaaa ctacgatttg
     4321 tttttttctg tttttttcca tgttttatat tatttatatt tgtttgacga gaatatcttt
     4381 atttgccgac aaaggtacat aactaaagtt tcccacccaa ataaatagat agaaaaataa
     4441 cagtttgtcg aattttcttt gtaaattagt aatcgctaaa gaactgattt tggggatccc
     4501 cgggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4561 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4621 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     4681 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     4741 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     4801 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     4861 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     4921 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4981 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5041 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     5101 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     5161 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     5221 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     5281 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     5341 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5401 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5461 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5521 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5581 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5641 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5701 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5761 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5821 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5881 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5941 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     6001 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     6061 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     6121 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6181 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6241 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6301 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6361 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6421 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6481 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6541 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6601 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6661 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6721 cggagcctat gga
//
