LOCUS       V004972                 6806 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004972
VERSION     V004972
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6806)
  AUTHORS   Liu M, Wang M.
  TITLE     Development of a plasmid-based methods for expression and antisense
            gene knockdown in Riemerella anatipestifer and application
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6806)
  AUTHORS   Liu M, Wang M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-MAR-2016) Institute of Preventive Veterinary Medicine,
            Sichuan Agricultural University, No. 211, Huimin Road, Chengdu,
            Sichuan 611130, China
REFERENCE   3  (bases 1 to 6806)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6806)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-MAR-2016) Institute of Preventive Veterinary Medicine, Sichuan
            Agricultural University, No. 211, Huimin Road, Chengdu, Sichuan
            611130, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    290..311
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        326..356
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    364..380
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     388..404
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2763..2780
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(3452..4414)
                     /gene="cfxA"
                     /label="Beta-lactamase"
                     /note="Beta-lactamase from Phocaeicola vulgatus.
                     Accession#: P30899"
     primer_bind     complement(4594..4610)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5084..5188
                     /label="AmpR promoter"
     CDS             5189..6046
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6220..6806
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
       61 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      121 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      181 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      241 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      301 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      361 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      421 ttgggctatt taggcattag ccctcttttt aacctgactt aaaaaataat taaatcataa
      481 gatattaaat atcaagtgat tatgatttac tatgctatac ataatatcac ccagtgttag
      541 aaaattcttg ctatgctttt tgtttgtttt ttttcattcg tgaactttta gaatactttt
      601 aaaataaact caaatttata aactattgat tttcaatatt atacaaaacc aaacttatta
      661 acattaaact tatttctatt acctttaaaa taaactacta aaagacaaat ctaccaatcc
      721 tcggaactat ttaataaata ggaagttata taaaaataat gaacttttag aatactttat
      781 tagtcttttt agctccaaaa atgaactttt agaatacttt ttttgaactt ttagaatact
      841 tttttaaact tgctttttag taagttcatt ttttatatat ttgtattcta aaagtgcatt
      901 ttatattcat aactctctat atatggcaaa agtagataat aaactaatgt ttcagtccta
      961 tatttttact acggctaaat atgatttttc agtctatgaa aagcgtatac tatataggca
     1021 aatagaaata gaacaggcat tgctaaataa tgaggcgttg aaagattgta tcaaaataga
     1081 tactaaccta tggggggata aaaaatatac aattccaatt tctatgctgt tagctaatga
     1141 tgaagataaa aattattata aaattaaaaa agcattcaaa gacctaaaat caaagaacat
     1201 tgagtacgaa gatgaaaaag aatacgcttg ctttggtgtt attgataagt tttggattga
     1261 taaatatgga agaaatgtta cttggcaatc tgacaaaaga attgttgaag cggtaatgga
     1321 ttttacaaga ggatggagaa aatatgaatt gaagattgct atgcagtttg agactattta
     1381 tgcaatgcgt ttttacgaac tgatagctaa taaaaccaaa ccaataacat atgaagtgtc
     1441 atttcttgaa aaaatgtttc aattggaaaa taagtacagg aaccctcagg gtaatcttaa
     1501 tatcagtatg tttaaacgcc gtgtattaga ccccgctaaa aacgagttag ataaatgttc
     1561 tccatacact tttgattata ctctttcaaa ggataaaaaa attatttctc taattcctat
     1621 tttccaagaa caatttgcag atgaaagtat gaagatgatc aaaatagaag aggaaaaaga
     1681 tatacactct gtattaactc aaagagaaat tgatgttttt acaaacgaat ttggttttac
     1741 acaacaaggt ttattaaaca actatgccct attcaaagat tgtaaggcta cacttccatc
     1801 aaactattct ttcttccttt ttcaagagat aagaaaaagt cggactaaga aaggaaaaat
     1861 atctcctgct tatgtgattg ggattataag aaatatgctt aaagatttca aaaaagaaca
     1921 acaataggct tatgaaaaaa tatacaacaa aagaaatcgc agagcttttt ggcgtcagcg
     1981 aacggacaat ccagcgacac ataacgacac taagcgacaa catatcaaat accgacagaa
     2041 agggcttttc aataccagaa gacattgttt tctttcttgc cgagcgacac cattacgaca
     2101 ttttagcgac aacgaacgac aacgaacgac aggataaaaa tgaagaattt cctcatattg
     2161 aatacttcac agaagaggaa taccaagagt ttaaaaaaag gatcacagag tacccttttt
     2221 tgaaagagca aattaaactg tctaatgaac atttggatac tctaaaaaca caaatagaat
     2281 atttcagact atcttacaat aagcaattgg atattcacga aaaattaatc ggagcgttcc
     2341 gagaaagaaa ctttattgaa gctaaagaaa aggggatgga taaatagctc ctaataatta
     2401 aagaagtatt agaaaaaaaa tgctatataa aaacttaatc gagctctata cgagatctgt
     2461 tccaatgcat tggctgcagg tcgacgctca tcggtatttg caacatcata gaaattgcat
     2521 acctttgttc ctcggttata tgtttgctca tctgcaactt ttttttcttt ggacggacaa
     2581 ttaaagcaaa gatagcaaac tttatccatt cagagtgaga gaaaggggga cattgtctct
     2641 ctttcctctc tgaaaaataa atgtttttat tgcttattat ccgcacccaa aaagttgcat
     2701 ttataagttg aactcaagaa gtattcacct gtaagaagtt acccatggct ctagaactcg
     2761 agcatcatca tcatcatcat tgaactagtg gagttgcttc tcttttcggg agtggaaaac
     2821 tgaaagaact ggaacgtgcc aacgaaaaac tgcaagacga ggtttcaaaa cggaacacca
     2881 atattgaaaa attgcagagc caagtacagc agatgcagaa acagcatgat acgcaaatcc
     2941 acaatctcag agaaatgcac aggcaggaac ttgacatgaa agaaaaagaa ctgtcacggc
     3001 tcgccagaat catagacaag gctttcaggt ggtttccgat gttcagggaa atgctgcgca
     3061 tggaaaagtt ttgtgccatg ctgggattct ctaaagaaat gactgaaagt cttatagtca
     3121 aaaaagaagc cctgaaatgt agcggtaaaa tctattccga gcaacacagg cggaactttg
     3181 atataaagga tgatatttta agggtggaaa atgaccctga cgatgaaagc aggctgaacc
     3241 tgacaataaa caggaagccg attgccgact ggttcaggga gcaatggcac aggcttagat
     3301 atggagcaag agtgccgcaa caggaagaaa gaaaaagtag aggattcaaa ttataataga
     3361 agcaatttga ttagtaatct aaaagcactc cgataacgat tagagtgctt ttagattgtt
     3421 tatcattaat tatcaaagca agtgcagttt aagattttac tgaagtttgc attaataaag
     3481 aatatactac agctgatata tgcgcaacat attgtgacgc ttgtgattta tttcccttga
     3541 aatccttaac aaataccgct aaggtataac tgatattatt aggcagacat atataggcaa
     3601 catcattgtg agctgcaaga acaccatttt cattaacata acctgaacct gtcttatgcg
     3661 ctataacaac cccttcttta tcaagaagtg gagctgctat cctatctaca cctgttttgc
     3721 attcttttaa cgtattctta atgaaacttt gtttctcatc atcgataaga ccttcagtaa
     3781 acaaacgatt catcaacatt gcagcaccaa gaggagatgt atagttagag taagccttgt
     3841 tatggtcagc cgacatttcc tcttccgtat aagctatctg aaaacttgaa cgaggaatga
     3901 gtgtggctat aaaactatct gtttgagcga cattaaccat atccttaaac ataaggttgc
     3961 ttgcattgtt gtcactctga gtaagagtat aacgcagcaa atctctcact gtcaatgata
     4021 tgactggccc tgaataatct ttcagcatag gactccaagt ctttgggtca agtttatccc
     4081 tatttatatt tactaaggta tcaagtgaaa ttcctttatt gtcaaagtca ttacaaagag
     4141 ctaatgcctg atgaacctta aacacactca tcataggata aacactctta ttattgacct
     4201 taaccgtatc tctgttatta acaataaccg ccacaccaat ttcgccagga caagctgaga
     4261 caatttgaga aatgctatca gtcaaaacat ttgttaaagg aggatttgcg ctatcttttg
     4321 tcgctgattt atggaacaat gaaaatacca agatgaaaat gcaaactaaa gctatactca
     4381 aaactacgat ttgttttttt ctgttttttt ccatgtttta tattatttat atttgtttga
     4441 cgagaatatc tttatttgcc gacaaaggta cataactaaa gtttcccacc caaataaata
     4501 gatagaaaaa taacagtttg tcgaattttc tttgtaaatt agtaatcgct aaagaactga
     4561 ttttggggat ccccgggtac cgagctcgaa ttcactggcc gtcgttttac aacgtcgtga
     4621 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     4681 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     4741 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     4801 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     4861 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     4921 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     4981 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5041 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5101 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5161 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5221 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5281 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5341 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5401 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5461 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5521 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5581 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5641 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     5701 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     5761 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     5821 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     5881 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     5941 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6001 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6061 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6121 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6181 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6241 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6301 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6361 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6421 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6481 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6541 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6601 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6661 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     6721 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     6781 ctcgtcaggg gggcggagcc tatgga
//
