LOCUS       40924_28476        7232 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLM53, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Novel Escherichia coli strains to detect protein-protein 
            interactions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7232)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7232)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-AUG-1999) Biochemistry and Biophysics, Texas A "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2650..2838
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    2943..3083
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3269..3857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4031..4888)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4889..4980)
                     /label=AmpR promoter
     terminator      complement(4999..5026)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(5118..5204)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(5503..6159)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     regulatory      complement(6231..6285)
                     /gene="cat"
                     /label=pL promoter
                     /note="pL promoter"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /regulatory_class="promoter"
     misc_binding    complement(6240..6256)
                     /gene="cat"
                     /label=O-L1 repressor binding site
                     /bound_moiety="O-L1 repressor"
     misc_binding    complement(6264..6280)
                     /gene="cat"
                     /label=O-L2 repressor binding site
                     /bound_moiety="O-L2 repressor"
     promoter        6369..6397
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             6586..7116
                     /label=GmR
                     /note="gentamycin acetyltransferase"
ORIGIN
        1 taagcggaga tcgcctagtg attttaaact attgctggca gcattcttga gtccaatata
       61 aaagtattgt gtaccttttg ctgggtcagg ttgttcttta ggaggagtaa aaggatcaaa
      121 tgcactaaac gaaactgaaa caagcgatcg aaaatatccc tttgggattc ttgactcgat
      181 aagtctatta ttttcagaga aaaaatattc attgttttct gggttggtga ttgcaccaat
      241 cattccattc aaaattgttg ttttaccaca cccattccgc ccgataaaag catgaatgtt
      301 cgtgctgggc atagaattaa ccgtcacctc aaaaggtata gttaaatcac tgaatccggg
      361 agcacttttt ctattaaatg aaaagtggaa atctgacaat tctggcaaac catttaacac
      421 acgtgcgaac tgtccatgaa tttctgaaag agttacccct ctaagtaatg aggtgttaag
      481 gacgctttca ttttcaatgt cggctaatcg atttggccat actactaaat cctgaatagc
      541 tttaagaagg ttatgtttaa aaccatcgct taatttgctg agattaacat agtagtcaat
      601 gctttcacct aaggaaaaaa acatttcagg gagttgactg aattttttat ctattaatga
      661 ataagtgctt acttcttctt tttgacctac aaaaccaatt ttaacatttc cgatatcgca
      721 tttttcacca tgctcatcaa agacagtaag ataaaacatt gtaacaaagg aatagtcatt
      781 ccaaccatct gctcgtagga atgccttatt tttttctact gcaggaatat acccgcctct
      841 ttcaataaca ctaaactcca acatatagta acccttaatt ttattaaaat aaccgcaatt
      901 tatttggcgg caacacagga tctctctttt aagttactct ctattacata cgttttccat
      961 ctaaaaatta gtagtattga acttaacggg gcatcgtatt gtagttttcc atatttagct
     1021 ttctgcttcc ttttggataa cccactgtta ttcatgttgc atggtgcact gtttatacca
     1081 acgatatagt ctattaatgc atatatagta tcgccgaacg attagctctt caggcttctg
     1141 aagaagcgtt tcaagtacta ataagccgat agatagccac ggacttcgta gccatttttc
     1201 ataagtgtta acttccgctc ctcgctcata acagacattc actacagtta tggcggaaag
     1261 gtatgcatgc tgggtgtggg gaagtcgtga aagaaaagaa gtcagctgcg tcgtttgaca
     1321 tcactgctat cttcttactg gttatgcagg tcgtagtggg tggcacacaa agcttggctg
     1381 caggtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
     1441 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
     1501 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga
     1561 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg
     1621 gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg
     1681 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga
     1741 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
     1801 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt
     1861 cgatcactgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg
     1921 ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg
     1981 gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt
     2041 caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa
     2101 cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc
     2161 gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
     2221 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
     2281 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
     2341 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg
     2401 gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa
     2461 gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg
     2521 tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag
     2581 catcctctct cgtttcatcg gtatcattac ccccatgaac agaaattccc ccttacacgg
     2641 aggcatcaag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc
     2701 cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc
     2761 tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt
     2821 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     2881 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     2941 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     3001 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     3061 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     3121 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     3181 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     3241 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     3301 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3361 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3421 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3481 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3541 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3601 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3661 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     3721 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3781 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3841 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3901 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3961 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     4021 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     4081 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     4141 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     4201 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     4261 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     4321 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     4381 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     4441 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     4501 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     4561 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     4621 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     4681 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     4741 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     4801 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     4861 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     4921 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     4981 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt aatttgatgc
     5041 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cgcaacgttc
     5101 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
     5161 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct ggcagttccc
     5221 tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt
     5281 cggcatgggg tcaggtggga ccaccgcgct actgccgcca ggcaaattct gttttatcag
     5341 accgcttctg cgttctgatt taatctgtat caggctgaaa atcttctctc atccgccaaa
     5401 acagaagctt cgacgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc
     5461 accaggcgtt taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc
     5521 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
     5581 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     5641 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     5701 gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa
     5761 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     5821 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     5881 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     5941 aaactccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     6001 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     6061 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     6121 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     6181 aaatctcgtc gaagctagtc gacatggtac cggtcagtgc gtcctgctga tgtgctcagt
     6241 atcaccgcca gtggtattta tgtcaacacc gccagagata atttagaatt cgaaatgcct
     6301 cgacttcgct gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac
     6361 gaacccagtt gacataagcc tgttcggttc gtaaactgta atgcaagtag cgtatgcgct
     6421 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     6481 tcatggcttg ttatgactgt ttttttgtac agtctatgcc tcgggcatcc aagcagcaag
     6541 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagc
     6601 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taggtggctc aagtatgggc
     6661 atcattcgca catgtaggct cggccctgac caagtcaaat ccatgcgggc tgctcttgat
     6721 cttttcggtc gtgagttcgg agacgtagcc acctactccc aacatcagcc ggactccgat
     6781 tacctcggga acttgctccg tagtaagaca ttcatcgcgc ttgctgcctt cgaccaagaa
     6841 gcggttgttg gcgctctcgc ggcttacgtt ctgcccaggt ttgagcagcc gcgtagtgag
     6901 atctatatct atgatctcgc agtctccggc gagcaccgga ggcagggcat tgccaccgcg
     6961 ctcatcaatc tcctcaagca tgaggccaac gcgcttggtg cttatgtgat ctacgtgcaa
     7021 gcagattacg gtgacgatcc cgcagtggct ctctatacaa agttgggcat acgggaagaa
     7081 gtgatgcact ttgatatcga cccaagtacc gccacctaac aattcgttca agccgagatc
     7141 ggcttcccgg ccgcggagtt gttcggtaaa ttgtcacaac gccgcggccg caaagcgctc
     7201 cggcttaact caggcgttag tcccatgaat tc
//
