LOCUS       62056_14350        4680 bp DNA     circular SYN 28-MAR-2021
DEFINITION  Cloning vector pLM459-mTurquoise2, complete sequence.
ACCESSION   MT737964
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Emrich-Mills TZ, Yates G, Barrett J, Grouneva I, Lau CS, Walker CE, 
            Kwok TK, Davey JW, Johnson MP, Mackinder LCM.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2020) Molecular Biology and Biotechnology, 
            University of Sheffield, Western Bank, Sheffield, South Yorkshire 
            S10 2TN, United Kingdom
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (08-JUL-2020) Molecular Biology and Biotechnology, University of 
            Sheffield, Western Bank, Sheffield, South Yorkshire S10 2TN, United 
            Kingdom"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             28..744
                     /label=mTurquoise2
                     /note="enhanced monomeric variant of CFP (Goedhart et al., 
                     2012)"
     CDS             754..780
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             784..810
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             814..840
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             join(1583..1750,1896..2108)
                     /codon_start=1
                     /transl_table=11
                     /product="Ble"
                     /label=Ble
                     /note="zeocin resistance marker"
                     /protein_id="QTE34489.1"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPW
                     GREFALRDPAGNCVHFVAEEQD"
     rep_origin      2417..2962
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     CDS             complement(3061..3873)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             4323..4625
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
ORIGIN
        1 ggagatctgg gtggctccgg cggccggatg gtgagcaagg gcgaggagct gttcaccggg
       61 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
      121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
      181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgtcctgggg cgtgcagtgc
      241 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
      301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
      361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
      421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actactttag cgacaacgtc
      481 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
      541 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
      601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac
      661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      721 ctcggcatgg acgagctgta caagggcggt ggctacccct acgacgtgcc cgactacgcc
      781 tcctacccct acgacgtgcc cgactacgcg ggctacccgt atgacgtccc cgattacgcg
      841 taagcgttct ggcagcagct ggaccgcctg taccatggag aagagcttta cttgccggga
      901 tggccgattt cgctgattga tacgggatcg gagctcggag gctttcgcgc taggggctag
      961 gcgaagggca gtggtgacca gggtcggtgt ggggtcggcc cacggtcaat tagccacagg
     1021 aggatcaggg ggaggtaggc acgtcgactt ggtttgcgac cccgcagttt tggcggacgt
     1081 gctgttgtag atgttagcgt gtgcgtgagc cagtggccaa cgtgccacac ccattgagaa
     1141 gaccaaccaa cttactggca atatctgcca atgccatact gcatgtaatg gccaggccat
     1201 gtgagagttt gccgtgcctg cgcgcgcccc gggggcgaag cttggtaccg ctagcttcga
     1261 tatcaagctt ctttcttgcg ctatgacact tccagcaaaa ggtagggcgg gctgcgagac
     1321 ggcttcccgg cgctgcatgc aacaccgatg atgcttcgac cccccgaagc tccttcgggg
     1381 ctgcatgggc gctccgatgc cgctccaggg cgagcgctgt ttaaatagcc aggcccccga
     1441 ttgcaaagac attatagcga gctaccaaag ccatattcaa acacctagat cactaccact
     1501 tctacacagg ccactcgagc ttgtgatcgc actccgctaa gggggcgcct cttcctcttc
     1561 gtttcagtca caacccgcaa acatggccaa gctgaccagc gccgttccgg tgctcaccgc
     1621 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     1681 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     1741 ccaggaccag gtgagtcgac gagcaagccc ggcggatcag gcagcgtgct tgcagatttg
     1801 acttgcaacg cccgcattgt gtcgacgaag gcttttggct cctctgtcgc tgtctcaagc
     1861 agcatctaac cctgcgtcgc cgtttccatt tgcaggacca ggtggtgccg gacaacaccc
     1921 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     1981 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     2041 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     2101 aggactgata aggatccccg ctccgtgtaa atggaggcgc tcgttgatct gagccttgcc
     2161 ccctgacgaa cggcggtgga tggaagatac tgctctcaag tgctgaagcg gtagcttagc
     2221 tccccgtttc gtgctgatca gtctttttca acacgtaaaa agcggaggag ttttgcaatt
     2281 ttgttggttg taacgatcct ccgttgattt tggcctcttt ctccatgggc gggctgggcg
     2341 tatttgaagc gtagggataa cagggtaatt gcactgaaat ctagaaatat tttatctgat
     2401 taataagatg atcttcttga gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg
     2461 aaaaaaccgc cttgcagggc ggtttttcga aggttctctg agctaccaac tctttgaacc
     2521 gaggtaactg gcttggagga gcgcagtcac caaaacttgt cctttcagtt tagccttaac
     2581 cggcgcatga cttcaagact aactcctcta aatcaattac cagtggctgc tgccagtggt
     2641 gcttttgcat gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2701 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa
     2761 ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa
     2821 ccgaaaggca ggaacaggag agcgcacgag ggagccgcca gggggaaacg cctggtatct
     2881 ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc
     2941 aggggggcgg agcctatgga aaaaccaggt ggcacttttc ggggaaatgt gcgcggaacc
     3001 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa ttaattctta
     3061 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc
     3121 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag
     3181 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat
     3241 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga
     3301 atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc
     3361 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc
     3421 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg
     3481 caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc
     3541 ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc
     3601 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag
     3661 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa
     3721 ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt
     3781 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct
     3841 agagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta
     3901 agcagacagt tttattgttc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3961 cgtcagaccc cgtagaaaag atcaaaggat cttcgttaac taaaagccag ataacagtat
     4021 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt
     4081 atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct
     4141 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca
     4201 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc
     4261 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg
     4321 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg
     4381 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac
     4441 gtctgctgtc agataaagtc ccccgtgaac tttacccggt ggtgcatatc ggggatgaaa
     4501 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag
     4561 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
     4621 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgttaac
//
