LOCUS       40924_28471        5042 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLM3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Lambda repressor fusion vectors to map protein-protein interactions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Marino-Ramirez L, Hu JC.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 5042)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5042)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-AUG-1999) Biochemistry and Biophysics, Texas A "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      6..47
                     /regulatory_class="promoter"
     CDS             73..462
                     /codon_start=1
                     /product="lambda repressor protein"
                     /label=lambda repressor protein
                     /note="DNA binding domain"
                     /experiment="experimental evidence, no additional details
                     recorded"
                     /protein_id="AAF01197.1"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAER"
     terminator      571..618
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             2103..2291
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    2396..2536
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2722..3310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3421..3934
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(3979..4836)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4837..4941)
                     /label=AmpR promoter
ORIGIN
        1 aattctaggc tttatgcttt atgcttccgg ctcgtacatt gtgtggaggt acctctagac
       61 aggaaacagc gtatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca
      121 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct
      181 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat
      241 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa
      301 tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg
      361 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca
      421 cctaagctta gaacctttac caaaggtgat gcggagagat aggtgtcgac gcccgggcat
      481 gcttcgaaga tcttaattaa ttaaggatcc ggcgctgcta acaaagcccg aaaggaagct
      541 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
      601 gtcttgaggg gttttttgct gaaaggagga actatatgcc cggatatcgt ccattccgac
      661 agcatcgcca gtcactatgg cgtgctgcta gcgctatatg cgttgatgca atttctatgc
      721 gcacccgttc tcggagcact gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg
      781 ctacttggag ccactatcga ctacgcgatc atggcgacca cacccgtcct gtggatctcg
      841 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
      901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
      961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
     1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
     1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
     1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
     1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcac
     1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
     1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg
     1561 cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat ctcgggcagc
     1621 gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac ccggctaggc
     1681 tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg aacgtgaagc
     1741 gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt cggtttccgt
     1801 gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt ccggatctgc
     1861 atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac gaagcgctgg
     1921 cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag ttgtttaccc
     1981 tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc
     2041 tctcgtttca tcggtatcat tacccccatg aacagaaatt cccccttaca cggaggcatc
     2101 aagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat
     2161 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat
     2221 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg
     2281 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     2341 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     2401 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     2461 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     2521 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2581 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2641 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2701 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2761 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2821 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2881 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     2941 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3001 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3061 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3121 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     3181 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3241 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3301 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3361 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3421 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     3481 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     3541 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     3601 gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc
     3661 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     3721 gactcttgtt ccaaactgga acaacactca accctatctc gggctattct tttgatttat
     3781 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     3841 acgcgaattt taacaaaata ttaacgttta caatttaaat atttgcttat acaatcttcc
     3901 tgtttttggg gcttttctga ttatcaaccg gggtaaatca atctaaagta tatatgagta
     3961 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     4021 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     4081 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     4141 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     4201 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     4261 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     4321 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     4381 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     4441 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4501 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4561 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     4621 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4681 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4741 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4801 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4861 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4921 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     4981 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     5041 ag
//
