LOCUS       40924_28456        6561 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLM006-mCherry, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, 
            Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, 
            Roth R, Sommer F, Geimer S, Muhlhaus T, Schroda M, Goodenough U, 
            Stitt M, Griffiths H, Jonikas MC.
  TITLE     A repeat protein links Rubisco to form the eukaryotic 
            carbon-concentrating organelle
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2016) In press
  PUBMED    27166422
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Mackinder LCM., Meyer MT, Mettler-Altmann T, Chen V, Mitchell M, 
            Caspari O, Freeman Rosenzweig E, Pallesen L, Reeves G, Itakura A, 
            Roth R, Sommer F, Geimer S, Muhlhaus T, Schroda M, Goodenough U, 
            Stitt M, Griffiths H, Jonikas MC.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2016) Department of Plant Biology, Carnegie 
            Institution of Washington, 260 Panama Street, Stanford, CA 94305, 
            USA
REFERENCE   3  (bases 1 to 6561)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6561)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2016) Department of Plant Biology, Carnegie Institution of 
            Washington, 260 Panama Street, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(423..509,655..1566)
                     /codon_start=1
                     /product="APHVII"
                     /label=APHVII
                     /note="hygromycin resistance marker"
                     /protein_id="ANF29840.1"
                     /translation="MTQESLLLLDRIDSDDSYASLRNDQEFWEPLARRALEELGLPVPP
                     VLRVPGESTNPVLVGEPGPVIKLFGEHWCGPESLASESEAYAVLADAPVPVPRLLGRGE
                     LRPGTGAWPWPYLVMSRMTGTTWRSAMDGTTDRNALLALARELGRVLGRLHRVPLTGNT
                     VLTPHSEVFPELLRERRAATVEDHRGWGYLSPRLLDRLEDWLPDVDTLLAGREPRFVHG
                     DLHGTNIFVDLAATEVTGIVDFTDVYAGDSRYSLVQLHLNAFRGDREILAALLDGAQWK
                     RTEDFARELLAFTFLHDFEVFEETPLDLSGFTDPEELAQFLWGPPDTAPGA"
     promoter        complement(1827..1845)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2644..3351
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYR"
     CDS             3364..3381
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     rep_origin      complement(4742..5330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5504..6361)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6362..6466)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaattcga tatcaagctt ctttcttgcg
      121 ctatgacact tccagcaaaa ggtagggcgg gctgcgagac ggcttcccgg cgctgcatgc
      181 aacaccgatg atgcttcgac cccccgaagc tccttcgggg ctgcatgggc gctccgatgc
      241 cgctccaggg cgagcgctgt ttaaatagcc aggcccccga ttgcaaagac attatagcga
      301 gctaccaaag ccatattcaa acacctagat cactaccact tctacacagg ccactcgagc
      361 ttgtgatcgc actccgctaa gggggcgcct cttcctcttc gtttcagtca caacccgcaa
      421 acatgacaca agaatccctg ttacttctcg accgtattga ttcggatgat tcctacgcga
      481 gcctgcggaa cgaccaggaa ttctgggagg tgagtcgacg agcaagcccg gcggatcagg
      541 cagcgtgctt gcagatttga cttgcaacgc ccgcattgtg tcgacgaagg cttttggctc
      601 ctctgtcgct gtctcaagca gcatctaacc ctgcgtcgcc gtttccattt gcagccgctg
      661 gcccgccgag ccctggagga gctcgggctg ccggtgccgc cggtgctgcg ggtgcccggc
      721 gagagcacca accccgtact ggtcggcgag cccggcccgg tgatcaagct gttcggcgag
      781 cactggtgcg gtccggagag cctcgcgtcg gagtcggagg cgtacgcggt cctggcggac
      841 gccccggtgc cggtgccccg cctcctcggc cgcggcgagc tgcggcccgg caccggagcc
      901 tggccgtggc cctacctggt gatgagccgg atgaccggca ccacctggcg gtccgcgatg
      961 gacggcacga ccgaccggaa cgcgctgctc gccctggccc gcgaactcgg ccgggtgctc
     1021 ggccggctgc acagggtgcc gctgaccggg aacaccgtgc tcacccccca ttccgaggtc
     1081 ttcccggaac tgctgcggga acgccgcgcg gcgaccgtcg aggaccaccg cgggtggggc
     1141 tacctctcgc cccggctgct ggaccgcctg gaggactggc tgccggacgt ggacacgctg
     1201 ctggccggcc gcgaaccccg gttcgtccac ggcgacctgc acgggaccaa catcttcgtg
     1261 gacctggccg cgaccgaggt caccgggatc gtcgacttca ccgacgtcta tgcgggagac
     1321 tcccgctaca gcctggtgca actgcatctc aacgccttcc ggggcgaccg cgagatcctg
     1381 gccgcgctgc tcgacggggc gcagtggaag cggaccgagg acttcgcccg cgaactgctc
     1441 gccttcacct tcctgcacga cttcgaggtg ttcgaggaga ccccgctgga tctctccggc
     1501 ttcaccgatc cggaggaact ggcgcagttc ctctgggggc cgccggacac cgcccccggc
     1561 gcctgataag gatccccgct ccgtgtaaat ggaggcgctc gttgatctga gccttgcccc
     1621 ctgacgaacg gcggtggatg gaagatactg ctctcaagtg ctgaagcggt agcttagctc
     1681 cccgtttcgt gctgatcagt ctttttcaac acgtaaaaag cggaggagtt ttgcaatttt
     1741 gttggttgta acgatcctcc gttgattttg gcctctttct ccatgggcgg gctgggcgta
     1801 tttgaagcgg gtaccagctt ttgttccctt tagtgagggt taattgcgac caatcgtcac
     1861 acgagccctc gtcagaaaca cgtctccgcc acgctctccc tctcacggcc gaccccgcag
     1921 cccttttgcc ctttcctagg ccaccgacag gacccaggcg ctctcagcat gcctcaacaa
     1981 cccgtactcg tgccagcggt gcccttgtgc tggtgatcgc ttggaagcgc atgcgaagac
     2041 gaaggggcgg agcaggcggc ctggctgttc gaagggctcg ccgccagttc gggtgccttt
     2101 ctccacgcgc gcctccacac ctaccgatgc gtgaaggcag gcaaatgctc atgtttgccc
     2161 gaactcggtg tccttaaaaa gccgcttctt gtcgtcgttc cgagacatgt tagcagatcg
     2221 cagtgccacc tttcctgacg cgctcggccc catattcgga cgcaattgtc atttgtagca
     2281 caattggagc aaatctggcg aggcagtagg cttttaagtt gcaaggcgag agagcaaagt
     2341 gggacgcggc gtgattattg gtatttacgc gacggcccgg cgcgttagcg gcccttcccc
     2401 caggccaggg acgattatgt atcaatattg ttgcgttcgg gcactcgtgc gagggctcct
     2461 gcgggctggg gagggggatc tgggaattgg aggtacgacc gagatggctt gctcgggggg
     2521 aggtttcctc gccgagcaag ccagggttag gtgttgcgct cttgacacgt tgtgcattct
     2581 aggaccccac tgctactcac aacaagccca gttaacggag atctgggtgg ctccggcggc
     2641 cggatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     2701 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     2761 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     2821 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     2881 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     2941 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     3001 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     3061 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     3121 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     3181 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     3241 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     3301 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacag atctggcggt
     3361 ggccaccacc atcaccacca cggagatctc taactgcacg aattctcagg tcgggacatc
     3421 cccgaccagg ggcggcgggg atgttgctgg gccgatggaa agtagcaacc cagccagcgg
     3481 cttccagcgc actccagctg ctcacggttg cgacattgcg cgtgcacgct tgcgcgtccc
     3541 tcactcggcc agcttgtcgc cgcagacatc cctagcattg tgcggactgc ggtcgtcagt
     3601 tagcgtagtg gcggggctca aagcgtgatg cagctggtgg ctgattgcat gtgctacata
     3661 tgctgttatg ttttgcatga acttcgatgc attggatgct gggtgcacgc gtttgcatgt
     3721 gtttgtgccg gcatgctgcc gtcgtcggcc gtacgtttac gtttctgtgt gccggggtct
     3781 ttatttccgc ctgcagaccg acgtcggttc cactagttct agatggcagc agctggaccg
     3841 cctgtaccat ggagaagagc tttacttgcc gggatggccg atttcgctga ttgatacggg
     3901 atcggagctc ggaggctttc gcgctagggg ctaggcgaag ggcagtggtg accagggtcg
     3961 gtgtggggtc ggcccacggt caattagcca caggaggatc agggggaggt aggcacgtcg
     4021 acttggtttg cgaccccgca gttttggcgg acgtgctgtt gtagatgtta gcgtgtgcgt
     4081 gagccagtgg ccaacgtgcc acacccattg agaagaccaa ccaacttact ggcaatatct
     4141 gccaatgcca tactgcatgt aatggccagg ccatgtgaga gtttgccgtg cctgcgcgcg
     4201 ccccgggggc gaagcttggt accgctagcg tcagctgaac tcctgctgcc acgctgacag
     4261 agacgcgccc atgacgtagc gccgactgct gctcctctgc tcgtggagct ctgaatctgc
     4321 tgccggctgc ccccgccccc gctgctgagg ctgaatctac tccgctctcc accccagtcc
     4381 caggaagaga ggcgcattta cttcgcacag acgttacagc acacccttga tcatcatcag
     4441 ctgctcttcc ctgccgctgc aacacgcccc gcgcgatatc ccagtcggga aacctgtcgt
     4501 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     4561 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4621 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4681 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4741 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4801 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4861 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4921 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4981 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     5041 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5101 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5161 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5221 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5281 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5341 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5401 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     5461 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     5521 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     5581 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     5641 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     5701 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     5761 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     5821 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     5881 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     5941 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6001 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     6061 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     6121 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     6181 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     6241 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     6301 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     6361 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     6421 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     6481 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     6541 gtatcacgag gccctttcgt c
//
