LOCUS       40924_28461        7686 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLM006-RBCS1-mCherry, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, 
            Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, 
            Roth R, Sommer F, Geimer S, Muhlhaus T, Schroda M, Goodenough U, 
            Stitt M, Griffiths H, Jonikas MC.
  TITLE     A repeat protein links Rubisco to form the eukaryotic 
            carbon-concentrating organelle
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2016) In press
  PUBMED    27166422
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   Mackinder LCM., Meyer MT, Mettler-Altmann T, Chen V, Mitchell M, 
            Caspari O, Freeman Rosenzweig E, Pallesen L, Reeves G, Itakura A, 
            Roth R, Sommer F, Geimer S, Muhlhaus T, Schroda M, Goodenough U, 
            Stitt M, Griffiths H, Jonikas MC.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2016) Department of Plant Biology, Carnegie 
            Institution of Washington, 260 Panama Street, Stanford, CA 94305, 
            USA
REFERENCE   3  (bases 1 to 7686)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7686)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2016) Department of Plant Biology, Carnegie Institution of 
            Washington, 260 Panama Street, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(423..509,655..1566)
                     /codon_start=1
                     /product="APHVII"
                     /label=APHVII
                     /note="hygromycin resistance marker"
                     /protein_id="ANF29844.1"
                     /translation="MTQESLLLLDRIDSDDSYASLRNDQEFWEPLARRALEELGLPVPP
                     VLRVPGESTNPVLVGEPGPVIKLFGEHWCGPESLASESEAYAVLADAPVPVPRLLGRGE
                     LRPGTGAWPWPYLVMSRMTGTTWRSAMDGTTDRNALLALARELGRVLGRLHRVPLTGNT
                     VLTPHSEVFPELLRERRAATVEDHRGWGYLSPRLLDRLEDWLPDVDTLLAGREPRFVHG
                     DLHGTNIFVDLAATEVTGIVDFTDVYAGDSRYSLVQLHLNAFRGDREILAALLDGAQWK
                     RTEDFARELLAFTFLHDFEVFEETPLDLSGFTDPEELAQFLWGPPDTAPGA"
     promoter        complement(1827..1845)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3769..4476
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYR"
     CDS             4489..4506
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     rep_origin      complement(5867..6455)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6629..7486)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7487..7591)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaattcga tatcaagctt ctttcttgcg
      121 ctatgacact tccagcaaaa ggtagggcgg gctgcgagac ggcttcccgg cgctgcatgc
      181 aacaccgatg atgcttcgac cccccgaagc tccttcgggg ctgcatgggc gctccgatgc
      241 cgctccaggg cgagcgctgt ttaaatagcc aggcccccga ttgcaaagac attatagcga
      301 gctaccaaag ccatattcaa acacctagat cactaccact tctacacagg ccactcgagc
      361 ttgtgatcgc actccgctaa gggggcgcct cttcctcttc gtttcagtca caacccgcaa
      421 acatgacaca agaatccctg ttacttctcg accgtattga ttcggatgat tcctacgcga
      481 gcctgcggaa cgaccaggaa ttctgggagg tgagtcgacg agcaagcccg gcggatcagg
      541 cagcgtgctt gcagatttga cttgcaacgc ccgcattgtg tcgacgaagg cttttggctc
      601 ctctgtcgct gtctcaagca gcatctaacc ctgcgtcgcc gtttccattt gcagccgctg
      661 gcccgccgag ccctggagga gctcgggctg ccggtgccgc cggtgctgcg ggtgcccggc
      721 gagagcacca accccgtact ggtcggcgag cccggcccgg tgatcaagct gttcggcgag
      781 cactggtgcg gtccggagag cctcgcgtcg gagtcggagg cgtacgcggt cctggcggac
      841 gccccggtgc cggtgccccg cctcctcggc cgcggcgagc tgcggcccgg caccggagcc
      901 tggccgtggc cctacctggt gatgagccgg atgaccggca ccacctggcg gtccgcgatg
      961 gacggcacga ccgaccggaa cgcgctgctc gccctggccc gcgaactcgg ccgggtgctc
     1021 ggccggctgc acagggtgcc gctgaccggg aacaccgtgc tcacccccca ttccgaggtc
     1081 ttcccggaac tgctgcggga acgccgcgcg gcgaccgtcg aggaccaccg cgggtggggc
     1141 tacctctcgc cccggctgct ggaccgcctg gaggactggc tgccggacgt ggacacgctg
     1201 ctggccggcc gcgaaccccg gttcgtccac ggcgacctgc acgggaccaa catcttcgtg
     1261 gacctggccg cgaccgaggt caccgggatc gtcgacttca ccgacgtcta tgcgggagac
     1321 tcccgctaca gcctggtgca actgcatctc aacgccttcc ggggcgaccg cgagatcctg
     1381 gccgcgctgc tcgacggggc gcagtggaag cggaccgagg acttcgcccg cgaactgctc
     1441 gccttcacct tcctgcacga cttcgaggtg ttcgaggaga ccccgctgga tctctccggc
     1501 ttcaccgatc cggaggaact ggcgcagttc ctctgggggc cgccggacac cgcccccggc
     1561 gcctgataag gatccccgct ccgtgtaaat ggaggcgctc gttgatctga gccttgcccc
     1621 ctgacgaacg gcggtggatg gaagatactg ctctcaagtg ctgaagcggt agcttagctc
     1681 cccgtttcgt gctgatcagt ctttttcaac acgtaaaaag cggaggagtt ttgcaatttt
     1741 gttggttgta acgatcctcc gttgattttg gcctctttct ccatgggcgg gctgggcgta
     1801 tttgaagcgg gtaccagctt ttgttccctt tagtgagggt taattgcgac caatcgtcac
     1861 acgagccctc gtcagaaaca cgtctccgcc acgctctccc tctcacggcc gaccccgcag
     1921 cccttttgcc ctttcctagg ccaccgacag gacccaggcg ctctcagcat gcctcaacaa
     1981 cccgtactcg tgccagcggt gcccttgtgc tggtgatcgc ttggaagcgc atgcgaagac
     2041 gaaggggcgg agcaggcggc ctggctgttc gaagggctcg ccgccagttc gggtgccttt
     2101 ctccacgcgc gcctccacac ctaccgatgc gtgaaggcag gcaaatgctc atgtttgccc
     2161 gaactcggtg tccttaaaaa gccgcttctt gtcgtcgttc cgagacatgt tagcagatcg
     2221 cagtgccacc tttcctgacg cgctcggccc catattcgga cgcaattgtc atttgtagca
     2281 caattggagc aaatctggcg aggcagtagg cttttaagtt gcaaggcgag agagcaaagt
     2341 gggacgcggc gtgattattg gtatttacgc gacggcccgg cgcgttagcg gcccttcccc
     2401 caggccaggg acgattatgt atcaatattg ttgcgttcgg gcactcgtgc gagggctcct
     2461 gcgggctggg gagggggatc tgggaattgg aggtacgacc gagatggctt gctcgggggg
     2521 aggtttcctc gccgagcaag ccagggttag gtgttgcgct cttgacacgt tgtgcattct
     2581 aggaccccac tgctactcac aacaagccca gttatggccg ccgtcattgc caagtcctcc
     2641 gtctccgcgg ccgtggctcg cccggcccgc tccagcgtgc gccccatggc cgcgctgaag
     2701 cccgccgtca aggctgcccc cgtggctgcc ccggctcagg ccaaccagat gatggtctgg
     2761 accccggtca acaacaagtg agtcgacgag caagcgcagc ccgaaggata gggattctgc
     2821 aagtcgcgac gcaattgctt ggggcccagc ctgctgcctc acatcgcacg tgctctgcca
     2881 cttctaacga gtactggtca atcgcgtgat cgcaggatgt tcgagacctt ctcctacctg
     2941 cctcctctga ccgacgagca gatcgccgcc caggtcgact acatcgtcgc caacggtgag
     3001 cacggcttgg tgccgtgggc atatgtagct gggctgtgtt tgagagctag ttaatacgat
     3061 ctagggcgta gctgcagact gcgacattac gatcgctcga agcttgagat tagcttgttc
     3121 gttgtctgct gtatagtttg cctgtcatcc gcccttatct gggatagccc tggactgctt
     3181 gcgtcggcca cttatcttac ccccttcgtg ctgtcgcttg caggctggat cccctgcctg
     3241 gagttcgctg aggccgacaa ggcctacgtg tccaacgagt cggccatccg cttcggcagc
     3301 gtgtcttgcg taagtcggag cgagagccag acgggtccac tgtggcactg ggttagcttg
     3361 gcaccgggac agcgcctatc tcaccgcggg gaactgacgc atacccctgc tcgtgcttca
     3421 gcacggaaaa gcaaggggcc caattccatc tttggtggtt ctgtgcgctg gtgactgaac
     3481 ctcttctccc tcccatttcc cgtgcgcccg cagctgtact acgacaaccg ctactggacc
     3541 atgtggaagc tgcccatgtt cggctgccgc gaccccatgc aggtgctgcg cgagatcgtc
     3601 gcctgcacca aggccttccc cgatgcctac gtgcgcctgg tggccttcga caaccagaag
     3661 caggtgcaga tcatgggctt cctggtccag cgccccaaga ctgcccgcga cttccagccc
     3721 gccaacaagc gctccgtgaa cggagatctg ggtggctccg gcggccggat ggtgagcaag
     3781 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     3841 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     3901 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     3961 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     4021 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     4081 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     4141 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     4201 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     4261 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     4321 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     4381 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     4441 cgccactcca ccggcggcat ggacgagctg tacagatctg gcggtggcca ccaccatcac
     4501 caccacggag atctctaact gcacgaattc tcaggtcggg acatccccga ccaggggcgg
     4561 cggggatgtt gctgggccga tggaaagtag caacccagcc agcggcttcc agcgcactcc
     4621 agctgctcac ggttgcgaca ttgcgcgtgc acgcttgcgc gtccctcact cggccagctt
     4681 gtcgccgcag acatccctag cattgtgcgg actgcggtcg tcagttagcg tagtggcggg
     4741 gctcaaagcg tgatgcagct ggtggctgat tgcatgtgct acatatgctg ttatgttttg
     4801 catgaacttc gatgcattgg atgctgggtg cacgcgtttg catgtgtttg tgccggcatg
     4861 ctgccgtcgt cggccgtacg tttacgtttc tgtgtgccgg ggtctttatt tccgcctgca
     4921 gaccgacgtc ggttccacta gttctagatg gcagcagctg gaccgcctgt accatggaga
     4981 agagctttac ttgccgggat ggccgatttc gctgattgat acgggatcgg agctcggagg
     5041 ctttcgcgct aggggctagg cgaagggcag tggtgaccag ggtcggtgtg gggtcggccc
     5101 acggtcaatt agccacagga ggatcagggg gaggtaggca cgtcgacttg gtttgcgacc
     5161 ccgcagtttt ggcggacgtg ctgttgtaga tgttagcgtg tgcgtgagcc agtggccaac
     5221 gtgccacacc cattgagaag accaaccaac ttactggcaa tatctgccaa tgccatactg
     5281 catgtaatgg ccaggccatg tgagagtttg ccgtgcctgc gcgcgccccg ggggcgaagc
     5341 ttggtaccgc tagcgtcagc tgaactcctg ctgccacgct gacagagacg cgcccatgac
     5401 gtagcgccga ctgctgctcc tctgctcgtg gagctctgaa tctgctgccg gctgcccccg
     5461 cccccgctgc tgaggctgaa tctactccgc tctccacccc agtcccagga agagaggcgc
     5521 atttacttcg cacagacgtt acagcacacc cttgatcatc atcagctgct cttccctgcc
     5581 gctgcaacac gccccgcgcg atatcccagt cgggaaacct gtcgtgccag ctgcattaat
     5641 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     5701 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5761 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5821 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5881 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5941 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6001 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6061 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6121 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6181 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6241 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6301 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6361 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6421 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6481 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6541 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6601 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6661 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6721 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6781 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6841 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6901 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6961 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7021 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7081 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7141 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7201 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7261 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7321 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7381 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7441 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7501 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7561 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     7621 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     7681 ttcgtc
//
