LOCUS       40924_28446        7260 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLM006-EPYC1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7260)
  AUTHORS   Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, 
            Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, 
            Roth R, Sommer F, Geimer S, Muhlhaus T, Schroda M, Goodenough U, 
            Stitt M, Griffiths H, Jonikas MC.
  TITLE     A repeat protein links Rubisco to form the eukaryotic 
            carbon-concentrating organelle
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2016) In press
  PUBMED    27166422
REFERENCE   2  (bases 1 to 7260)
  AUTHORS   Mackinder LCM., Meyer MT, Mettler-Altmann T, Chen V, Mitchell M, 
            Caspari O, Freeman Rosenzweig E, Pallesen L, Reeves G, Itakura A, 
            Roth R, Sommer F, Geimer S, Muhlhaus T, Schroda M, Goodenough U, 
            Stitt M, Griffiths H, Jonikas MC.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2016) Department of Plant Biology, Carnegie 
            Institution of Washington, 260 Panama Street, Stanford, CA 94305, 
            USA
REFERENCE   3  (bases 1 to 7260)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7260)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2016) Department of Plant Biology, Carnegie Institution of 
            Washington, 260 Panama Street, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(423..509,655..1566)
                     /codon_start=1
                     /product="APHVII"
                     /label=APHVII
                     /note="hygromycin resistance marker"
                     /protein_id="ANF29836.1"
                     /translation="MTQESLLLLDRIDSDDSYASLRNDQEFWEPLARRALEELGLPVPP
                     VLRVPGESTNPVLVGEPGPVIKLFGEHWCGPESLASESEAYAVLADAPVPVPRLLGRGE
                     LRPGTGAWPWPYLVMSRMTGTTWRSAMDGTTDRNALLALARELGRVLGRLHRVPLTGNT
                     VLTPHSEVFPELLRERRAATVEDHRGWGYLSPRLLDRLEDWLPDVDTLLAGREPRFVHG
                     DLHGTNIFVDLAATEVTGIVDFTDVYAGDSRYSLVQLHLNAFRGDREILAALLDGAQWK
                     RTEDFARELLAFTFLHDFEVFEETPLDLSGFTDPEELAQFLWGPPDTAPGA"
     promoter        complement(1827..1845)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             join(2614..2679,2931..2992,3255..4092)
                     /codon_start=1
                     /product="EPYC1"
                     /label=EPYC1
                     /note="EPYC1 with additional 4 amino acids at C terminal"
                     /protein_id="ANF29835.1"
                     /translation="MATISSMRVGAASRVVVSGRVKTVKVAARGSWRESSTATVQASRA
                     SSATNRVSPTRSVLPANWRQELESLRNGNGSSSAASSAPAPARSSSASWRDAAPASSAP
                     ARSSSASKKAVTPSRSALPSNWKQELESLRSSSPAPASSAPAPARSSSASWRDAAPASS
                     APARSSSSKKAVTPSRSALPSNWKQELESLRSSSPAPASSAPAPARSSSASWRDAAPAS
                     SAPARSSSASKKAVTPSRSALPSNWKQELESLRSNSPAPASSAPAPARSSSASWRDAPA
                     SSSSSSADKAGTNPWTGKSKPEIKRTALPADWRKGLNGDL"
     rep_origin      complement(5441..6029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6203..7060)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7061..7165)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaattcga tatcaagctt ctttcttgcg
      121 ctatgacact tccagcaaaa ggtagggcgg gctgcgagac ggcttcccgg cgctgcatgc
      181 aacaccgatg atgcttcgac cccccgaagc tccttcgggg ctgcatgggc gctccgatgc
      241 cgctccaggg cgagcgctgt ttaaatagcc aggcccccga ttgcaaagac attatagcga
      301 gctaccaaag ccatattcaa acacctagat cactaccact tctacacagg ccactcgagc
      361 ttgtgatcgc actccgctaa gggggcgcct cttcctcttc gtttcagtca caacccgcaa
      421 acatgacaca agaatccctg ttacttctcg accgtattga ttcggatgat tcctacgcga
      481 gcctgcggaa cgaccaggaa ttctgggagg tgagtcgacg agcaagcccg gcggatcagg
      541 cagcgtgctt gcagatttga cttgcaacgc ccgcattgtg tcgacgaagg cttttggctc
      601 ctctgtcgct gtctcaagca gcatctaacc ctgcgtcgcc gtttccattt gcagccgctg
      661 gcccgccgag ccctggagga gctcgggctg ccggtgccgc cggtgctgcg ggtgcccggc
      721 gagagcacca accccgtact ggtcggcgag cccggcccgg tgatcaagct gttcggcgag
      781 cactggtgcg gtccggagag cctcgcgtcg gagtcggagg cgtacgcggt cctggcggac
      841 gccccggtgc cggtgccccg cctcctcggc cgcggcgagc tgcggcccgg caccggagcc
      901 tggccgtggc cctacctggt gatgagccgg atgaccggca ccacctggcg gtccgcgatg
      961 gacggcacga ccgaccggaa cgcgctgctc gccctggccc gcgaactcgg ccgggtgctc
     1021 ggccggctgc acagggtgcc gctgaccggg aacaccgtgc tcacccccca ttccgaggtc
     1081 ttcccggaac tgctgcggga acgccgcgcg gcgaccgtcg aggaccaccg cgggtggggc
     1141 tacctctcgc cccggctgct ggaccgcctg gaggactggc tgccggacgt ggacacgctg
     1201 ctggccggcc gcgaaccccg gttcgtccac ggcgacctgc acgggaccaa catcttcgtg
     1261 gacctggccg cgaccgaggt caccgggatc gtcgacttca ccgacgtcta tgcgggagac
     1321 tcccgctaca gcctggtgca actgcatctc aacgccttcc ggggcgaccg cgagatcctg
     1381 gccgcgctgc tcgacggggc gcagtggaag cggaccgagg acttcgcccg cgaactgctc
     1441 gccttcacct tcctgcacga cttcgaggtg ttcgaggaga ccccgctgga tctctccggc
     1501 ttcaccgatc cggaggaact ggcgcagttc ctctgggggc cgccggacac cgcccccggc
     1561 gcctgataag gatccccgct ccgtgtaaat ggaggcgctc gttgatctga gccttgcccc
     1621 ctgacgaacg gcggtggatg gaagatactg ctctcaagtg ctgaagcggt agcttagctc
     1681 cccgtttcgt gctgatcagt ctttttcaac acgtaaaaag cggaggagtt ttgcaatttt
     1741 gttggttgta acgatcctcc gttgattttg gcctctttct ccatgggcgg gctgggcgta
     1801 tttgaagcgg gtaccagctt ttgttccctt tagtgagggt taattgcgac caatcgtcac
     1861 acgagccctc gtcagaaaca cgtctccgcc acgctctccc tctcacggcc gaccccgcag
     1921 cccttttgcc ctttcctagg ccaccgacag gacccaggcg ctctcagcat gcctcaacaa
     1981 cccgtactcg tgccagcggt gcccttgtgc tggtgatcgc ttggaagcgc atgcgaagac
     2041 gaaggggcgg agcaggcggc ctggctgttc gaagggctcg ccgccagttc gggtgccttt
     2101 ctccacgcgc gcctccacac ctaccgatgc gtgaaggcag gcaaatgctc atgtttgccc
     2161 gaactcggtg tccttaaaaa gccgcttctt gtcgtcgttc cgagacatgt tagcagatcg
     2221 cagtgccacc tttcctgacg cgctcggccc catattcgga cgcaattgtc atttgtagca
     2281 caattggagc aaatctggcg aggcagtagg cttttaagtt gcaaggcgag agagcaaagt
     2341 gggacgcggc gtgattattg gtatttacgc gacggcccgg cgcgttagcg gcccttcccc
     2401 caggccaggg acgattatgt atcaatattg ttgcgttcgg gcactcgtgc gagggctcct
     2461 gcgggctggg gagggggatc tgggaattgg aggtacgacc gagatggctt gctcgggggg
     2521 aggtttcctc gccgagcaag ccagggttag gtgttgcgct cttgacacgt tgtgcattct
     2581 aggaccccac tgctactcac aacaagccca gttatggcca ctatctcgtc gatgcgcgtt
     2641 ggcgctgctt cccgcgtggt cgtctctggc cgcgtgaagg tgaggcactt gatcgcactt
     2701 cttcttcagt agtgtcaagg cggcaggttc atggcgcggc ttgataggcg accagagcga
     2761 cttgcaagct tggcctcggc gcaaatgccg ttacacgacc gcgcttgctt ggcttcgcgc
     2821 gccgagcagc gcagctgcat ggcaatggtt gttatgatca tagtctccac cacgccttgc
     2881 tcttcagcct ttctgatgcg actgttactg tccttcgcgc gcccttgcag accgtgaagg
     2941 tcgccgcccg cggcagctgg cgcgagtctt ccactgccac cgtgcaggcc aggtgagcac
     3001 acttctgcag ctatgagatg catctgggtc cagcttaaag cggctcgcgt tgtgtggcgc
     3061 gccgcgatcc cttatccgct cgcctgccag ccgggccttt tcgcacttgt ttcctaagtc
     3121 aagttcgaac ctgcagctgg ctgtgcatat cttgctaagt gatagcgcgg ttgtacgcgg
     3181 tttgagtacg ctgctcaact ggtgtactga cacgtttgct tgccgtttcc cctggtgccc
     3241 cttcgcccct gcagccgcgc ctcgtcggcc accaaccgcg tgagccccac ccgctccgtc
     3301 ctgcccgcca actggcgcca ggagctggag agcctgcgca acggcaacgg ctcctcctcg
     3361 gctgcctcgt cggcccccgc cccggcccgc tcctcgtcgg ccagctggcg cgacgccgcc
     3421 ccggcctcgt cggcccctgc ccgctccagc tctgcctcca agaaggccgt gaccccgtcg
     3481 cgcagcgccc tgccctccaa ctggaagcag gagctggaga gcctgcgcag cagctccccc
     3541 gcccccgcct cgtcggcccc cgccccggcc cgctcctcgt cggccagctg gcgtgatgcc
     3601 gccccggcct cgtcggcccc cgcccgctcc agctcctcca agaaggctgt gaccccgtcg
     3661 cgcagcgccc tgccctccaa ctggaagcag gagctggaga gcctgcgcag cagctccccc
     3721 gcccccgcct cgtcggcccc tgccccggcc cgctcctcgt cggccagctg gcgtgacgcc
     3781 gccccggcct cgtcggcccc tgcccgctcc agctctgcct ccaagaaggc cgtgaccccg
     3841 tcgcgcagcg ccctgccctc caactggaag caggagctgg agagcctgcg cagcaactcc
     3901 cctgcccccg cctcgtcggc ccctgccccg gcccgctcct cgtcggccag ctggcgtgac
     3961 gcccccgcct cgagctccag ctcgagcgcc gacaaggccg gcaccaaccc ctggactggc
     4021 aagtccaagc ccgagatcaa gcgcaccgcc ctgcccgctg actggcgcaa gggcctgaac
     4081 ggagatctct aactgcacga attctcaggt cgggacatcc ccgaccaggg gcggcgggga
     4141 tgttgctggg ccgatggaaa gtagcaaccc agccagcggc ttccagcgca ctccagctgc
     4201 tcacggttgc gacattgcgc gtgcacgctt gcgcgtccct cactcggcca gcttgtcgcc
     4261 gcagacatcc ctagcattgt gcggactgcg gtcgtcagtt agcgtagtgg cggggctcaa
     4321 agcgtgatgc agctggtggc tgattgcatg tgctacatat gctgttatgt tttgcatgaa
     4381 cttcgatgca ttggatgctg ggtgcacgcg tttgcatgtg tttgtgccgg catgctgccg
     4441 tcgtcggccg tacgtttacg tttctgtgtg ccggggtctt tatttccgcc tgcagaccga
     4501 cgtcggttcc actagttcta gatggcagca gctggaccgc ctgtaccatg gagaagagct
     4561 ttacttgccg ggatggccga tttcgctgat tgatacggga tcggagctcg gaggctttcg
     4621 cgctaggggc taggcgaagg gcagtggtga ccagggtcgg tgtggggtcg gcccacggtc
     4681 aattagccac aggaggatca gggggaggta ggcacgtcga cttggtttgc gaccccgcag
     4741 ttttggcgga cgtgctgttg tagatgttag cgtgtgcgtg agccagtggc caacgtgcca
     4801 cacccattga gaagaccaac caacttactg gcaatatctg ccaatgccat actgcatgta
     4861 atggccaggc catgtgagag tttgccgtgc ctgcgcgcgc cccgggggcg aagcttggta
     4921 ccgctagcgt cagctgaact cctgctgcca cgctgacaga gacgcgccca tgacgtagcg
     4981 ccgactgctg ctcctctgct cgtggagctc tgaatctgct gccggctgcc cccgcccccg
     5041 ctgctgaggc tgaatctact ccgctctcca ccccagtccc aggaagagag gcgcatttac
     5101 ttcgcacaga cgttacagca cacccttgat catcatcagc tgctcttccc tgccgctgca
     5161 acacgccccg cgcgatatcc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     5221 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     5281 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5341 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5401 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5461 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5521 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5581 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5641 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5701 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5761 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5821 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     5881 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     5941 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6001 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6061 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6121 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6181 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6241 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6301 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6361 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6421 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6481 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     6541 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6601 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6661 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6721 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6781 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     6841 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     6901 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     6961 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7021 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7081 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7141 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     7201 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
