LOCUS       40924_28441        8025 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLM006-EPYC1-mCherry, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8025)
  AUTHORS   Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, 
            Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, 
            Roth R, Sommer F, Geimer S, Muhlhaus T, Schroda M, Goodenough U, 
            Stitt M, Griffiths H, Jonikas MC.
  TITLE     A repeat protein links Rubisco to form the eukaryotic 
            carbon-concentrating organelle
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2016) In press
  PUBMED    27166422
REFERENCE   2  (bases 1 to 8025)
  AUTHORS   Mackinder LCM., Meyer MT, Mettler-Altmann T, Chen V, Mitchell M, 
            Caspari O, Freeman Rosenzweig E, Pallesen L, Reeves G, Itakura A, 
            Roth R, Sommer F, Geimer S, Muhlhaus T, Schroda M, Goodenough U, 
            Stitt M, Griffiths H, Jonikas MC.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2016) Department of Plant Biology, Carnegie 
            Institution of Washington, 260 Panama Street, Stanford, CA 94305, 
            USA
REFERENCE   3  (bases 1 to 8025)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8025)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2016) Department of Plant Biology, Carnegie Institution of 
            Washington, 260 Panama Street, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(423..509,655..1566)
                     /codon_start=1
                     /product="APHVII"
                     /label=APHVII
                     /note="hygromycin resistance marker"
                     /protein_id="ANF29838.1"
                     /translation="MTQESLLLLDRIDSDDSYASLRNDQEFWEPLARRALEELGLPVPP
                     VLRVPGESTNPVLVGEPGPVIKLFGEHWCGPESLASESEAYAVLADAPVPVPRLLGRGE
                     LRPGTGAWPWPYLVMSRMTGTTWRSAMDGTTDRNALLALARELGRVLGRLHRVPLTGNT
                     VLTPHSEVFPELLRERRAATVEDHRGWGYLSPRLLDRLEDWLPDVDTLLAGREPRFVHG
                     DLHGTNIFVDLAATEVTGIVDFTDVYAGDSRYSLVQLHLNAFRGDREILAALLDGAQWK
                     RTEDFARELLAFTFLHDFEVFEETPLDLSGFTDPEELAQFLWGPPDTAPGA"
     promoter        complement(1827..1845)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             4108..4815
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYR"
     CDS             4828..4845
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     rep_origin      complement(6206..6794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6968..7825)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7826..7930)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaattcga tatcaagctt ctttcttgcg
      121 ctatgacact tccagcaaaa ggtagggcgg gctgcgagac ggcttcccgg cgctgcatgc
      181 aacaccgatg atgcttcgac cccccgaagc tccttcgggg ctgcatgggc gctccgatgc
      241 cgctccaggg cgagcgctgt ttaaatagcc aggcccccga ttgcaaagac attatagcga
      301 gctaccaaag ccatattcaa acacctagat cactaccact tctacacagg ccactcgagc
      361 ttgtgatcgc actccgctaa gggggcgcct cttcctcttc gtttcagtca caacccgcaa
      421 acatgacaca agaatccctg ttacttctcg accgtattga ttcggatgat tcctacgcga
      481 gcctgcggaa cgaccaggaa ttctgggagg tgagtcgacg agcaagcccg gcggatcagg
      541 cagcgtgctt gcagatttga cttgcaacgc ccgcattgtg tcgacgaagg cttttggctc
      601 ctctgtcgct gtctcaagca gcatctaacc ctgcgtcgcc gtttccattt gcagccgctg
      661 gcccgccgag ccctggagga gctcgggctg ccggtgccgc cggtgctgcg ggtgcccggc
      721 gagagcacca accccgtact ggtcggcgag cccggcccgg tgatcaagct gttcggcgag
      781 cactggtgcg gtccggagag cctcgcgtcg gagtcggagg cgtacgcggt cctggcggac
      841 gccccggtgc cggtgccccg cctcctcggc cgcggcgagc tgcggcccgg caccggagcc
      901 tggccgtggc cctacctggt gatgagccgg atgaccggca ccacctggcg gtccgcgatg
      961 gacggcacga ccgaccggaa cgcgctgctc gccctggccc gcgaactcgg ccgggtgctc
     1021 ggccggctgc acagggtgcc gctgaccggg aacaccgtgc tcacccccca ttccgaggtc
     1081 ttcccggaac tgctgcggga acgccgcgcg gcgaccgtcg aggaccaccg cgggtggggc
     1141 tacctctcgc cccggctgct ggaccgcctg gaggactggc tgccggacgt ggacacgctg
     1201 ctggccggcc gcgaaccccg gttcgtccac ggcgacctgc acgggaccaa catcttcgtg
     1261 gacctggccg cgaccgaggt caccgggatc gtcgacttca ccgacgtcta tgcgggagac
     1321 tcccgctaca gcctggtgca actgcatctc aacgccttcc ggggcgaccg cgagatcctg
     1381 gccgcgctgc tcgacggggc gcagtggaag cggaccgagg acttcgcccg cgaactgctc
     1441 gccttcacct tcctgcacga cttcgaggtg ttcgaggaga ccccgctgga tctctccggc
     1501 ttcaccgatc cggaggaact ggcgcagttc ctctgggggc cgccggacac cgcccccggc
     1561 gcctgataag gatccccgct ccgtgtaaat ggaggcgctc gttgatctga gccttgcccc
     1621 ctgacgaacg gcggtggatg gaagatactg ctctcaagtg ctgaagcggt agcttagctc
     1681 cccgtttcgt gctgatcagt ctttttcaac acgtaaaaag cggaggagtt ttgcaatttt
     1741 gttggttgta acgatcctcc gttgattttg gcctctttct ccatgggcgg gctgggcgta
     1801 tttgaagcgg gtaccagctt ttgttccctt tagtgagggt taattgcgac caatcgtcac
     1861 acgagccctc gtcagaaaca cgtctccgcc acgctctccc tctcacggcc gaccccgcag
     1921 cccttttgcc ctttcctagg ccaccgacag gacccaggcg ctctcagcat gcctcaacaa
     1981 cccgtactcg tgccagcggt gcccttgtgc tggtgatcgc ttggaagcgc atgcgaagac
     2041 gaaggggcgg agcaggcggc ctggctgttc gaagggctcg ccgccagttc gggtgccttt
     2101 ctccacgcgc gcctccacac ctaccgatgc gtgaaggcag gcaaatgctc atgtttgccc
     2161 gaactcggtg tccttaaaaa gccgcttctt gtcgtcgttc cgagacatgt tagcagatcg
     2221 cagtgccacc tttcctgacg cgctcggccc catattcgga cgcaattgtc atttgtagca
     2281 caattggagc aaatctggcg aggcagtagg cttttaagtt gcaaggcgag agagcaaagt
     2341 gggacgcggc gtgattattg gtatttacgc gacggcccgg cgcgttagcg gcccttcccc
     2401 caggccaggg acgattatgt atcaatattg ttgcgttcgg gcactcgtgc gagggctcct
     2461 gcgggctggg gagggggatc tgggaattgg aggtacgacc gagatggctt gctcgggggg
     2521 aggtttcctc gccgagcaag ccagggttag gtgttgcgct cttgacacgt tgtgcattct
     2581 aggaccccac tgctactcac aacaagccca gttatggcca ctatctcgtc gatgcgcgtt
     2641 ggcgctgctt cccgcgtggt cgtctctggc cgcgtgaagg tgaggcactt gatcgcactt
     2701 cttcttcagt agtgtcaagg cggcaggttc atggcgcggc ttgataggcg accagagcga
     2761 cttgcaagct tggcctcggc gcaaatgccg ttacacgacc gcgcttgctt ggcttcgcgc
     2821 gccgagcagc gcagctgcat ggcaatggtt gttatgatca tagtctccac cacgccttgc
     2881 tcttcagcct ttctgatgcg actgttactg tccttcgcgc gcccttgcag accgtgaagg
     2941 tcgccgcccg cggcagctgg cgcgagtctt ccactgccac cgtgcaggcc aggtgagcac
     3001 acttctgcag ctatgagatg catctgggtc cagcttaaag cggctcgcgt tgtgtggcgc
     3061 gccgcgatcc cttatccgct cgcctgccag ccgggccttt tcgcacttgt ttcctaagtc
     3121 aagttcgaac ctgcagctgg ctgtgcatat cttgctaagt gatagcgcgg ttgtacgcgg
     3181 tttgagtacg ctgctcaact ggtgtactga cacgtttgct tgccgtttcc cctggtgccc
     3241 cttcgcccct gcagccgcgc ctcgtcggcc accaaccgcg tgagccccac ccgctccgtc
     3301 ctgcccgcca actggcgcca ggagctggag agcctgcgca acggcaacgg ctcctcctcg
     3361 gctgcctcgt cggcccccgc cccggcccgc tcctcgtcgg ccagctggcg cgacgccgcc
     3421 ccggcctcgt cggcccctgc ccgctccagc tctgcctcca agaaggccgt gaccccgtcg
     3481 cgcagcgccc tgccctccaa ctggaagcag gagctggaga gcctgcgcag cagctccccc
     3541 gcccccgcct cgtcggcccc cgccccggcc cgctcctcgt cggccagctg gcgtgatgcc
     3601 gccccggcct cgtcggcccc cgcccgctcc agctcctcca agaaggctgt gaccccgtcg
     3661 cgcagcgccc tgccctccaa ctggaagcag gagctggaga gcctgcgcag cagctccccc
     3721 gcccccgcct cgtcggcccc tgccccggcc cgctcctcgt cggccagctg gcgtgacgcc
     3781 gccccggcct cgtcggcccc tgcccgctcc agctctgcct ccaagaaggc cgtgaccccg
     3841 tcgcgcagcg ccctgccctc caactggaag caggagctgg agagcctgcg cagcaactcc
     3901 cctgcccccg cctcgtcggc ccctgccccg gcccgctcct cgtcggccag ctggcgtgac
     3961 gcccccgcct cgagctccag ctcgagcgcc gacaaggccg gcaccaaccc ctggactggc
     4021 aagtccaagc ccgagatcaa gcgcaccgcc ctgcccgctg actggcgcaa gggcctgaac
     4081 ggagatctgg gtggctccgg cggccggatg gtgagcaagg gcgaggagga taacatggcc
     4141 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     4201 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     4261 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     4321 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     4381 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     4441 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     4501 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     4561 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     4621 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     4681 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     4741 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     4801 gacgagctgt acagatctgg cggtggccac caccatcacc accacggaga tctctaactg
     4861 cacgaattct caggtcggga catccccgac caggggcggc ggggatgttg ctgggccgat
     4921 ggaaagtagc aacccagcca gcggcttcca gcgcactcca gctgctcacg gttgcgacat
     4981 tgcgcgtgca cgcttgcgcg tccctcactc ggccagcttg tcgccgcaga catccctagc
     5041 attgtgcgga ctgcggtcgt cagttagcgt agtggcgggg ctcaaagcgt gatgcagctg
     5101 gtggctgatt gcatgtgcta catatgctgt tatgttttgc atgaacttcg atgcattgga
     5161 tgctgggtgc acgcgtttgc atgtgtttgt gccggcatgc tgccgtcgtc ggccgtacgt
     5221 ttacgtttct gtgtgccggg gtctttattt ccgcctgcag accgacgtcg gttccactag
     5281 ttctagatgg cagcagctgg accgcctgta ccatggagaa gagctttact tgccgggatg
     5341 gccgatttcg ctgattgata cgggatcgga gctcggaggc tttcgcgcta ggggctaggc
     5401 gaagggcagt ggtgaccagg gtcggtgtgg ggtcggccca cggtcaatta gccacaggag
     5461 gatcaggggg aggtaggcac gtcgacttgg tttgcgaccc cgcagttttg gcggacgtgc
     5521 tgttgtagat gttagcgtgt gcgtgagcca gtggccaacg tgccacaccc attgagaaga
     5581 ccaaccaact tactggcaat atctgccaat gccatactgc atgtaatggc caggccatgt
     5641 gagagtttgc cgtgcctgcg cgcgccccgg gggcgaagct tggtaccgct agcgtcagct
     5701 gaactcctgc tgccacgctg acagagacgc gcccatgacg tagcgccgac tgctgctcct
     5761 ctgctcgtgg agctctgaat ctgctgccgg ctgcccccgc ccccgctgct gaggctgaat
     5821 ctactccgct ctccacccca gtcccaggaa gagaggcgca tttacttcgc acagacgtta
     5881 cagcacaccc ttgatcatca tcagctgctc ttccctgccg ctgcaacacg ccccgcgcga
     5941 tatcccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6001 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6061 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7921 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     7981 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
