LOCUS       40924_28431        9997 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pLM005-RCA1-Venus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Freeman Rosenzweig ES, Xu B, Kuhn Cuellar L, Marinez-Sanchez A, 
            Schaffer M, Strauss M, Cartwright HN, Forster F, Engel BD, Wingreen 
            NS, Mackinder LCM., Jonikas MC.
  TITLE     The pyrenoid is liquid-like and exhibits dynamic reorganization
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9997)
  AUTHORS   Mackinder LCM., Jonikas MC.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2017) Department of Biology, University of York, 
            Wentworth Way, York, North Yorkshire YO10 5DD, United Kingdom
REFERENCE   3  (bases 1 to 9997)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9997)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JAN-2017) Department of Biology, University of York, Wentworth 
            Way, York, North Yorkshire YO10 5DD, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3428..4144
                     /codon_start=1
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELIK"
     CDS             4196..4219
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     rep_origin      complement(5580..6168)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6342..7199)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7200..7304)
                     /label=AmpR promoter
     CDS             8183..8986
                     /codon_start=1
                     /product="APHVIII"
                     /label=APHVIII
                     /note="Paromomycin resistance marker"
                     /protein_id="AUR45267.1"
                     /translation="MDDALRALRGRYPGCEWVVVEDGASGAGVYRLRGGGRELFVKVAA
                     LGAGVGLLGEAERLVWLAEVGIPVPRVVEGGGDERVAWLVTEAVPGRPASARWPREQRL
                     DVAVALAGLARSLHALDWERCPFDRSLAVTVPQAARAVAEGSVDLEDLDEERKGWSGER
                     LLAELERTRPADEDLAVCHGDLCPDNVLLDPRTCEVTGLIDVGRVGRADRHSDLALVLR
                     ELAHEEDPWFGPECSAAFLREYGRGWDGAVSEEKLAFYRLLDEFF"
     promoter        complement(9311..9329)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cggggggagg tttcctcgcc gagcaagcca gggttaggtg ttgcgctctt gacacgttgt
       61 gcattctagg accccactgc tactcacaac aagcccagtt atgcaggtga gcgcagcggc
      121 tctttagtcg gcttgcaagc catctagata acccttgcta actttctttc ttgctgcctg
      181 ttcttcttcg ttcaaggtca ccatgaagag caggtgaggc ttgcagctga aatgcacgcg
      241 gctccgctcc gggttatcgt taacaacctt gcgcatgcgt agttcgcgaa gctcctggtc
      301 tgagccctga cctcccttct tgtttgccta tgcagcgccg tcagcggcca gcgcgtgggc
      361 ggtgcccgcg tcgccacccg tagcgtgcgc cgggtaagtt cgggctgctc cgtcggcatg
      421 ggaacgttac tgtgaacact gcgctgactg tttcccctct cgcttttgtc ccggcaggcg
      481 cagctgcagg ttgtggcctc tagccgcaag cagatgggcc gctggcggtc gatcgacgcg
      541 ggcgtcgacg cgtccgatga ccaggtgagc gcgttcgttg ctcgagcgct gactgctggg
      601 cccccatgtt cgtccccagt cagcgaccca gtgatgtcag agctgtgtag cagctaatgc
      661 ggcttgtttc cagcagcgcc tttgcctcgg ggtagtgctg acatcagcga ccggctcgcg
      721 cgtttttcgc tgggcagtcc gcgggagctc tcctcgaatg ctgatctgca gcttgtgggc
      781 gatggttatc aacaagcatt tgccaggacc attcgtgctc ttgccttatc cataagcgtg
      841 ccgatcgacc ttcccactgc atcatatcct cgcaacaacg cgcccagcta cagcaaggcg
      901 agactcacgt cagcacgcca atacgcagcc cgctcgcgtt tgcctagtgc ggtcgcctgc
      961 aagccctctc gcccagcttc accgcacggc accgttaggc caggccccga gcccctcggc
     1021 ctccctcctc gcccaccatt tctctctctc cttacgcccc ttttttaccc ttttccgccc
     1081 ctcctcgccc cttcttcgct cccccgctcg cagcaagaca tcactcgcgg ccgcgagatg
     1141 gtggacgacc tgttccaggg cggcttcggt gccggcggca cccacaacgc agtgctgtcc
     1201 agccaggagt acctgagcca gagccgcgcc tcgttcaaca acattgagga cggcttctac
     1261 atctcgcccg ctttcctgga caagatgacc atccacattg ccaagaactt catggtgaga
     1321 ggactttgag gcagtgccgc atgctctacc ctgggatcgc agcctccggc aactcggcac
     1381 ccgcctgacg tcaccgccag cggctgccta cctgcagcgc gcaacatgca ctttgctgcc
     1441 cgccctcgtc tccacccctg ctcctcacct ctccccaacc tgcacccctg catcgctccc
     1501 ctctatgcag gacctgccca agatcaaggt gcccctcatt ctgggtatct ggggtggcaa
     1561 gggccagggc aagaccttcc agtgcgcgct cgcctacaag aagctgggca ttgcccccat
     1621 cgtcatgtcc gctggtgagc tggagtccgg caacgccggt gagcccgcca agctgatccg
     1681 cacccgctac cgggaggcct ccgacatcat caagaagggc cgcatgtgct cgctgttcat
     1741 caacgatctg gacgccggtg ccggccgcat gggcgacacc acccagtaca ccgtgaacaa
     1801 ccagatggtg aacgccaccc tgatgaacat cgccgacaac ccgaccaacg tccaggtgcg
     1861 gctgcgtttt ttgcgtttgc gtttctgggc ttggctctct gcggttcctg tgtgacacgc
     1921 gcctgtgtgc gtgaggcggg ttgctgtggt agcggtgagc tagcaagccg atccgttccg
     1981 gaaaggtgta tgcgctgaag cgtttaggcg ggttgagcac taaagcattc ttctgacacc
     2041 attctgcttc ctccccctgc ccaccgccgc ccccgcaccg cagctgcccg gtgtgtacaa
     2101 gaacgaggag atccctcgcg tgcccattgt gtgcacgggc aacgacttct ccaccctgta
     2161 cgcgcccctg atccgcgatg gccgcatgga gaagtactac tggaacccca cccgcgagga
     2221 ccgcatcggc gtgtgcatgg gcatcttcca ggtgggcggt ttggggcgcc gcgtgcttgg
     2281 gatggggggg agggaacgag ggaatgaaag agagaggggg tgaataagcg ggagtagaac
     2341 gcggttgatt gccaggatgt tgctagctag cacaactggc tgctctgaag cacccgaata
     2401 cattacccta atcaggccaa gtcgtatcgc tacacgttct ctacctcgtc gcccactcgc
     2461 cagtgtgccg aaccctccgt atcttcaacc ccacccaaca ggaggacaac gttcagcgcc
     2521 gcgaggtgga gaacctggtg gacaccttcc ccggccagtc cattgacttc ttcggcgccc
     2581 tgcgtgcccg cgtgtacgac gacatggtgc gccagtggat caccgacacc ggcgtggaca
     2641 agatcggcca gcagctggtc aacgcccgcc agaaggtggc catgcccaag gtgagccatg
     2701 gaggacaagt gtgtgcgggg caggagcggc ggcgtttgaa cagggagaga catgtgggca
     2761 gcacttagga atgcaaggcc gtggcgggca gggcgggtgc ttggcggacc cggtttaggg
     2821 aggccgattg acgacggtat caaatcaagg gccggcagac atgcggcgag tgtaattcca
     2881 cccagctact gctttattga ggacaagtct tgcgttgcgg tgcccagggc ctgccctctc
     2941 accgcgtcgc tcccccagcg tgagcatgcc catctgtcaa ctgcgtgata ccgtgttact
     3001 catcgcgttc tcatcactcc atgatcacac aggtgtccat ggacctgaac gtgctgatca
     3061 agtacggcaa gtcgctggtg gacgagcagg agaacgtcaa gcgcgtgcag ctggccgatg
     3121 cctacctgtc gggtgagtct tggatttgag tttggttctg tttggtggtc ttgctgtgtt
     3181 cttgacacat ggggaatatt cagagtacct gccaatggtg ccgtgtcggg cccttgcaaa
     3241 gtggtttcgg ggcgcgcttc atagcgactg aatcgccagc cctctacaaa gcacaccgct
     3301 gacgcgtctc ctctactgcc cccgtttttt ttggaactcc tcaggcgccg agctggccgg
     3361 ccacggcggc tcttccctgc ccgaggccta cagccgcaac ggagatctgg gtggctccgg
     3421 cggccggatg gtgagcaagg gcgaggagct gttcaccggc gtggtgccca tcctggtgga
     3481 gctggacggc gacgtgaacg gccacaagtt cagcgtgagc ggcgagggcg agggcgacgc
     3541 cacctacggc aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg
     3601 gcccaccctg gtgaccaccc tgggctacgg cctgcagtgc ttcgcccgct accccgacca
     3661 catgaagcag cacgacttct tcaagagcgc catgcccgag ggctacgtgc aggagcgcac
     3721 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     3781 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3841 gggccacaag ctggagtaca actacaacag ccacaacgtg tacatcaccg ccgacaagca
     3901 gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca
     3961 gctggccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     4021 caaccactac ctgagctacc agagcgccct gagcaaggac cccaacgaga agcgcgacca
     4081 catggtgctg ctggagttcg tgaccgccgc cggcatcacc ctgggcatgg acgagctgat
     4141 caagggcggt ggcgactaca aggaccatga cggtgactat aaggatcacg acatcgacta
     4201 caaggacgat gacgacaagt aagatctcta actgcacgaa ttctcaggtc gggacatccc
     4261 cgaccagggg cggcggggat gttgctgggc cgatggaaag tagcaaccca gccagcggct
     4321 tccagcgcac tccagctgct cacggttgcg acattgcgcg tgcacgcttg cgcgtccctc
     4381 actcggccag cttgtcgccg cagacatccc tagcattgtg cggactgcgg tcgtcagtta
     4441 gcgtagtggc ggggctcaaa gcgtgatgca gctggtggct gattgcatgt gctacatatg
     4501 ctgttatgtt ttgcatgaac ttcgatgcat tggatgctgg gtgcacgcgt ttgcatgtgt
     4561 ttgtgccggc atgctgccgt cgtcggccgt acgtttacgt ttctgtgtgc cggggtcttt
     4621 atttccgcct gcagaccgac gtcggttcca ctagttctag atggcagcag ctggaccgcc
     4681 tgtaccatgg agaagagctt tacttgccgg gatggccgat ttcgctgatt gatacgggat
     4741 cggagctcgg aggctttcgc gctaggggct aggcgaaggg cagtggtgac cagggtcggt
     4801 gtggggtcgg cccacggtca attagccaca ggaggatcag ggggaggtag gcacgtcgac
     4861 ttggtttgcg accccgcagt tttggcggac gtgctgttgt agatgttagc gtgtgcgtga
     4921 gccagtggcc aacgtgccac acccattgag aagaccaacc aacttactgg caatatctgc
     4981 caatgccata ctgcatgtaa tggccaggcc atgtgagagt ttgccgtgcc tgcgcgcgcc
     5041 ccgggggcga agcttggtac cgctagcgtc agctgaactc ctgctgccac gctgacagag
     5101 acgcgcccat gacgtagcgc cgactgctgc tcctctgctc gtggagctct gaatctgctg
     5161 ccggctgccc ccgcccccgc tgctgaggct gaatctactc cgctctccac cccagtccca
     5221 ggaagagagg cgcatttact tcgcacagac gttacagcac acccttgatc atcatcagct
     5281 gctcttccct gccgctgcaa cacgccccgc gcgatatccc agtcgggaaa cctgtcgtgc
     5341 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5401 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5461 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5521 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5581 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5641 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5701 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5761 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5821 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5881 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5941 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6001 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6061 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6121 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6181 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6241 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6301 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6361 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6421 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6481 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6541 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6601 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6661 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6721 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6781 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6841 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6901 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6961 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7021 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7081 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7141 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7201 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7261 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7321 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     7381 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7441 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagatatc
     7501 cttgacatga ttggtgcgta tgtttgtatg aagctacagg actgatttgg cgggctatga
     7561 gggcggggga agctctggaa gggccgcgat ggggcgcgcg gcgtccagaa ggcgccatac
     7621 ggcccgctgg cggcacccat ccggtataaa agcccgcgac cccgaacggt gacctccact
     7681 ttcagcgaca aacgagcact tatacatacg cgactattct gccgctatac ataaccactc
     7741 agctagctta agatcccatc aagcttgcat gccgggcgcg ccagaaggag cgcagccaaa
     7801 ccaggatgat gtttgatggg gtatttgagc acttgcaacc cttatccgga agccccctgg
     7861 cccacaaagg ctaggcgcca atgcaagcag ttcgcatgca gcccctggag cggtgccctc
     7921 ctgataaacc ggccaggggg cctatgttct ttactttttt acaagagaag tcactcaaca
     7981 tcttaaaatg gccaggtgag tcgacgagca agcccggcgg atcaggcagc gtgcttgcag
     8041 atttgacttg caacgcccgc attgtgtcga cgaaggcttt tggctcctct gtcgctgtct
     8101 caagcagcat ctaaccctgc gtcgccgttt ccatttgcag gatggccact ccgccctccc
     8161 cggtgctgaa gaatttcgaa gcatggacga tgcgttgcgt gcactgcggg gtcggtatcc
     8221 cggttgtgag tgggttgttg tggaggatgg ggcctcgggg gctggtgttt atcggcttcg
     8281 gggtggtggg cgggagttgt ttgtcaaggt ggcagctctg ggggccgggg tgggcttgtt
     8341 gggtgaggct gagcggctgg tgtggttggc ggaggtgggg attcccgtac ctcgtgttgt
     8401 ggagggtggt ggggacgaga gggtcgcctg gttggtcacc gaagcggttc cggggcgtcc
     8461 ggccagtgcg cggtggccgc gggagcagcg gctggacgtg gcggtggcgc tcgcggggct
     8521 cgctcgttcg ctgcacgcgc tggactggga gcggtgtccg ttcgatcgca gtctcgcggt
     8581 gacggtgccg caggcggccc gtgctgtcgc tgaagggagc gtcgacttgg aggatctgga
     8641 cgaggagcgg aaggggtggt cgggggagcg gcttctcgcc gagctggagc ggactcggcc
     8701 tgcggacgag gatctggcgg tttgccacgg tgacctgtgc ccggacaacg tgctgctcga
     8761 ccctcgtacc tgcgaggtga ccgggctgat cgacgtgggg cgggtcggcc gtgcggaccg
     8821 gcactccgat ctcgcgctgg tgctgcgcga gctggcccac gaggaggacc cgtggttcgg
     8881 gccggagtgt tccgcggcgt tcctgcggga gtacgggcgc gggtgggatg gggcggtatc
     8941 ggaggaaaag ctggcgtttt accggctgtt ggacgagttc ttctgaggga cctgatggtg
     9001 ttggtggctg ggtagggttg cgtcgcgtgg gtgacagcac agtgtggacg ttgggatccc
     9061 cgctccgtgt aaatggaggc gctcgttgat ctgagccttg ccccctgacg aacggcggtg
     9121 gatggaagat actgctctca agtgctgaag cggtagctta gctccccgtt tcgtgctgat
     9181 cagtcttttt caacacgtaa aaagcggagg agttttgcaa ttttgttggt tgtaacgatc
     9241 ctccgttgat tttggcctct ttctccatgg gcgggctggg cgtatttgaa gcgggtacca
     9301 gcttttgttc cctttagtga gggttaattg cgaccaatcg tcacacgagc cctcgtcaga
     9361 aacacgtctc cgccacgctc tccctctcac ggccgacccc gcagcccttt tgccctttcc
     9421 taggccaccg acaggaccca ggcgctctca gcatgcctca acaacccgta ctcgtgccag
     9481 cggtgccctt gtgctggtga tcgcttggaa gcgcatgcga agacgaaggg gcggagcagg
     9541 cggcctggct gttcgaaggg ctcgccgcca gttcgggtgc ctttctccac gcgcgcctcc
     9601 acacctaccg atgcgtgaag gcaggcaaat gctcatgttt gcccgaactc ggtgtcctta
     9661 aaaagccgct tcttgtcgtc gttccgagac atgttagcag atcgcagtgc cacctttcct
     9721 gacgcgctcg gccccatatt cggacgcaat tgtcatttgt agcacaattg gagcaaatct
     9781 ggcgaggcag taggctttta agttgcaagg cgagagagca aagtgggacg cggcgtgatt
     9841 attggtattt acgcgacggc ccggcgcgtt agcggccctt cccccaggcc agggacgatt
     9901 atgtatcaat attgttgcgt tcgggcactc gtgcgagggc tcctgcgggc tggggagggg
     9961 gatctgggaa ttggaggtac gaccgagatg gcttgct
//
