LOCUS       40924_28421        8164 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLM005-EPYC1-Venus, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8164)
  AUTHORS   Mackinder LC, Meyer MT, Mettler-Altmann T, Chen VK, Mitchell MC, 
            Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, 
            Roth R, Sommer F, Geimer S, Muhlhaus T, Schroda M, Goodenough U, 
            Stitt M, Griffiths H, Jonikas MC.
  TITLE     A repeat protein links Rubisco to form the eukaryotic 
            carbon-concentrating organelle
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. (2016) In press
  PUBMED    27166422
REFERENCE   2  (bases 1 to 8164)
  AUTHORS   Mackinder LCM., Meyer MT, Mettler-Altmann T, Chen V, Mitchell M, 
            Caspari O, Freeman Rosenzweig E, Pallesen L, Reeves G, Itakura A, 
            Roth R, Sommer F, Geimer S, Muhlhaus T, Schroda M, Goodenough U, 
            Stitt M, Griffiths H, Jonikas MC.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2016) Department of Plant Biology, Carnegie 
            Institution of Washington, 260 Panama Street, Stanford, CA 94305, 
            USA
REFERENCE   3  (bases 1 to 8164)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8164)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A. (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2016) Department of Plant Biology, Carnegie Institution of 
            Washington, 260 Panama Street, Stanford, CA 94305, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8164
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1595..2311
                     /codon_start=1
                     /label=Venus
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELIK"
     CDS             2363..2386
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     rep_origin      complement(3747..4335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4509..5366)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5367..5471)
                     /label=AmpR promoter
     CDS             6350..7153
                     /codon_start=1
                     /product="APHVIII"
                     /label=APHVIII
                     /note="paromomycin resistance marker"
                     /protein_id="ANF29832.1"
                     /translation="MDDALRALRGRYPGCEWVVVEDGASGAGVYRLRGGGRELFVKVAA
                     LGAGVGLLGEAERLVWLAEVGIPVPRVVEGGGDERVAWLVTEAVPGRPASARWPREQRL
                     DVAVALAGLARSLHALDWERCPFDRSLAVTVPQAARAVAEGSVDLEDLDEERKGWSGER
                     LLAELERTRPADEDLAVCHGDLCPDNVLLDPRTCEVTGLIDVGRVGRADRHSDLALVLR
                     ELAHEEDPWFGPECSAAFLREYGRGWDGAVSEEKLAFYRLLDEFF"
     promoter        complement(7478..7496)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cggggggagg tttcctcgcc gagcaagcca gggttaggtg ttgcgctctt gacacgttgt
       61 gcattctagg accccactgc tactcacaac aagcccagtt atggccacta tctcgtcgat
      121 gcgcgttggc gctgcttccc gcgtggtcgt ctctggccgc gtgaaggtga ggcacttgat
      181 cgcacttctt cttcagtagt gtcaaggcgg caggttcatg gcgcggcttg ataggcgacc
      241 agagcgactt gcaagcttgg cctcggcgca aatgccgtta cacgaccgcg cttgcttggc
      301 ttcgcgcgcc gagcagcgca gctgcatggc aatggttgtt atgatcatag tctccaccac
      361 gccttgctct tcagcctttc tgatgcgact gttactgtcc ttcgcgcgcc cttgcagacc
      421 gtgaaggtcg ccgcccgcgg cagctggcgc gagtcttcca ctgccaccgt gcaggccagg
      481 tgagcacact tctgcagcta tgagatgcat ctgggtccag cttaaagcgg ctcgcgttgt
      541 gtggcgcgcc gcgatccctt atccgctcgc ctgccagccg ggccttttcg cacttgtttc
      601 ctaagtcaag ttcgaacctg cagctggctg tgcatatctt gctaagtgat agcgcggttg
      661 tacgcggttt gagtacgctg ctcaactggt gtactgacac gtttgcttgc cgtttcccct
      721 ggtgcccctt cgcccctgca gccgcgcctc gtcggccacc aaccgcgtga gccccacccg
      781 ctccgtcctg cccgccaact ggcgccagga gctggagagc ctgcgcaacg gcaacggctc
      841 ctcctcggct gcctcgtcgg cccccgcccc ggcccgctcc tcgtcggcca gctggcgcga
      901 cgccgccccg gcctcgtcgg cccctgcccg ctccagctct gcctccaaga aggccgtgac
      961 cccgtcgcgc agcgccctgc cctccaactg gaagcaggag ctggagagcc tgcgcagcag
     1021 ctcccccgcc cccgcctcgt cggcccccgc cccggcccgc tcctcgtcgg ccagctggcg
     1081 tgatgccgcc ccggcctcgt cggcccccgc ccgctccagc tcctccaaga aggctgtgac
     1141 cccgtcgcgc agcgccctgc cctccaactg gaagcaggag ctggagagcc tgcgcagcag
     1201 ctcccccgcc cccgcctcgt cggcccctgc cccggcccgc tcctcgtcgg ccagctggcg
     1261 tgacgccgcc ccggcctcgt cggcccctgc ccgctccagc tctgcctcca agaaggccgt
     1321 gaccccgtcg cgcagcgccc tgccctccaa ctggaagcag gagctggaga gcctgcgcag
     1381 caactcccct gcccccgcct cgtcggcccc tgccccggcc cgctcctcgt cggccagctg
     1441 gcgtgacgcc cccgcctcga gctccagctc gagcgccgac aaggccggca ccaacccctg
     1501 gactggcaag tccaagcccg agatcaagcg caccgccctg cccgctgact ggcgcaaggg
     1561 cctgaacgga gatctgggtg gctccggcgg ccggatggtg agcaagggcg aggagctgtt
     1621 caccggcgtg gtgcccatcc tggtggagct ggacggcgac gtgaacggcc acaagttcag
     1681 cgtgagcggc gagggcgagg gcgacgccac ctacggcaag ctgaccctga agctgatctg
     1741 caccaccggc aagctgcccg tgccctggcc caccctggtg accaccctgg gctacggcct
     1801 gcagtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agagcgccat
     1861 gcccgagggc tacgtgcagg agcgcaccat cttcttcaag gacgacggca actacaagac
     1921 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1981 cgacttcaag gaggacggca acatcctggg ccacaagctg gagtacaact acaacagcca
     2041 caacgtgtac atcaccgccg acaagcagaa gaacggcatc aaggccaact tcaagatccg
     2101 ccacaacatc gaggacggcg gcgtgcagct ggccgaccac taccagcaga acacccccat
     2161 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccaga gcgccctgag
     2221 caaggacccc aacgagaagc gcgaccacat ggtgctgctg gagttcgtga ccgccgccgg
     2281 catcaccctg ggcatggacg agctgatcaa gggcggtggc gactacaagg accatgacgg
     2341 tgactataag gatcacgaca tcgactacaa ggacgatgac gacaagtaag atctctaact
     2401 gcacgaattc tcaggtcggg acatccccga ccaggggcgg cggggatgtt gctgggccga
     2461 tggaaagtag caacccagcc agcggcttcc agcgcactcc agctgctcac ggttgcgaca
     2521 ttgcgcgtgc acgcttgcgc gtccctcact cggccagctt gtcgccgcag acatccctag
     2581 cattgtgcgg actgcggtcg tcagttagcg tagtggcggg gctcaaagcg tgatgcagct
     2641 ggtggctgat tgcatgtgct acatatgctg ttatgttttg catgaacttc gatgcattgg
     2701 atgctgggtg cacgcgtttg catgtgtttg tgccggcatg ctgccgtcgt cggccgtacg
     2761 tttacgtttc tgtgtgccgg ggtctttatt tccgcctgca gaccgacgtc ggttccacta
     2821 gttctagatg gcagcagctg gaccgcctgt accatggaga agagctttac ttgccgggat
     2881 ggccgatttc gctgattgat acgggatcgg agctcggagg ctttcgcgct aggggctagg
     2941 cgaagggcag tggtgaccag ggtcggtgtg gggtcggccc acggtcaatt agccacagga
     3001 ggatcagggg gaggtaggca cgtcgacttg gtttgcgacc ccgcagtttt ggcggacgtg
     3061 ctgttgtaga tgttagcgtg tgcgtgagcc agtggccaac gtgccacacc cattgagaag
     3121 accaaccaac ttactggcaa tatctgccaa tgccatactg catgtaatgg ccaggccatg
     3181 tgagagtttg ccgtgcctgc gcgcgccccg ggggcgaagc ttggtaccgc tagcgtcagc
     3241 tgaactcctg ctgccacgct gacagagacg cgcccatgac gtagcgccga ctgctgctcc
     3301 tctgctcgtg gagctctgaa tctgctgccg gctgcccccg cccccgctgc tgaggctgaa
     3361 tctactccgc tctccacccc agtcccagga agagaggcgc atttacttcg cacagacgtt
     3421 acagcacacc cttgatcatc atcagctgct cttccctgcc gctgcaacac gccccgcgcg
     3481 atatcccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3541 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3601 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3661 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3721 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3781 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3841 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3901 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3961 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4021 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4081 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4141 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4201 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4261 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4321 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4381 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4441 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4501 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4561 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4621 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4681 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4741 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4801 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4861 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4921 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4981 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5041 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5101 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5161 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5221 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5281 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5341 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5401 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5461 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     5521 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     5581 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     5641 gcggatgccg ggagcagaca agatatcctt gacatgattg gtgcgtatgt ttgtatgaag
     5701 ctacaggact gatttggcgg gctatgaggg cgggggaagc tctggaaggg ccgcgatggg
     5761 gcgcgcggcg tccagaaggc gccatacggc ccgctggcgg cacccatccg gtataaaagc
     5821 ccgcgacccc gaacggtgac ctccactttc agcgacaaac gagcacttat acatacgcga
     5881 ctattctgcc gctatacata accactcagc tagcttaaga tcccatcaag cttgcatgcc
     5941 gggcgcgcca gaaggagcgc agccaaacca ggatgatgtt tgatggggta tttgagcact
     6001 tgcaaccctt atccggaagc cccctggccc acaaaggcta ggcgccaatg caagcagttc
     6061 gcatgcagcc cctggagcgg tgccctcctg ataaaccggc cagggggcct atgttcttta
     6121 cttttttaca agagaagtca ctcaacatct taaaatggcc aggtgagtcg acgagcaagc
     6181 ccggcggatc aggcagcgtg cttgcagatt tgacttgcaa cgcccgcatt gtgtcgacga
     6241 aggcttttgg ctcctctgtc gctgtctcaa gcagcatcta accctgcgtc gccgtttcca
     6301 tttgcaggat ggccactccg ccctccccgg tgctgaagaa tttcgaagca tggacgatgc
     6361 gttgcgtgca ctgcggggtc ggtatcccgg ttgtgagtgg gttgttgtgg aggatggggc
     6421 ctcgggggct ggtgtttatc ggcttcgggg tggtgggcgg gagttgtttg tcaaggtggc
     6481 agctctgggg gccggggtgg gcttgttggg tgaggctgag cggctggtgt ggttggcgga
     6541 ggtggggatt cccgtacctc gtgttgtgga gggtggtggg gacgagaggg tcgcctggtt
     6601 ggtcaccgaa gcggttccgg ggcgtccggc cagtgcgcgg tggccgcggg agcagcggct
     6661 ggacgtggcg gtggcgctcg cggggctcgc tcgttcgctg cacgcgctgg actgggagcg
     6721 gtgtccgttc gatcgcagtc tcgcggtgac ggtgccgcag gcggcccgtg ctgtcgctga
     6781 agggagcgtc gacttggagg atctggacga ggagcggaag gggtggtcgg gggagcggct
     6841 tctcgccgag ctggagcgga ctcggcctgc ggacgaggat ctggcggttt gccacggtga
     6901 cctgtgcccg gacaacgtgc tgctcgaccc tcgtacctgc gaggtgaccg ggctgatcga
     6961 cgtggggcgg gtcggccgtg cggaccggca ctccgatctc gcgctggtgc tgcgcgagct
     7021 ggcccacgag gaggacccgt ggttcgggcc ggagtgttcc gcggcgttcc tgcgggagta
     7081 cgggcgcggg tgggatgggg cggtatcgga ggaaaagctg gcgttttacc ggctgttgga
     7141 cgagttcttc tgagggacct gatggtgttg gtggctgggt agggttgcgt cgcgtgggtg
     7201 acagcacagt gtggacgttg ggatccccgc tccgtgtaaa tggaggcgct cgttgatctg
     7261 agccttgccc cctgacgaac ggcggtggat ggaagatact gctctcaagt gctgaagcgg
     7321 tagcttagct ccccgtttcg tgctgatcag tctttttcaa cacgtaaaaa gcggaggagt
     7381 tttgcaattt tgttggttgt aacgatcctc cgttgatttt ggcctctttc tccatgggcg
     7441 ggctgggcgt atttgaagcg ggtaccagct tttgttccct ttagtgaggg ttaattgcga
     7501 ccaatcgtca cacgagccct cgtcagaaac acgtctccgc cacgctctcc ctctcacggc
     7561 cgaccccgca gcccttttgc cctttcctag gccaccgaca ggacccaggc gctctcagca
     7621 tgcctcaaca acccgtactc gtgccagcgg tgcccttgtg ctggtgatcg cttggaagcg
     7681 catgcgaaga cgaaggggcg gagcaggcgg cctggctgtt cgaagggctc gccgccagtt
     7741 cgggtgcctt tctccacgcg cgcctccaca cctaccgatg cgtgaaggca ggcaaatgct
     7801 catgtttgcc cgaactcggt gtccttaaaa agccgcttct tgtcgtcgtt ccgagacatg
     7861 ttagcagatc gcagtgccac ctttcctgac gcgctcggcc ccatattcgg acgcaattgt
     7921 catttgtagc acaattggag caaatctggc gaggcagtag gcttttaagt tgcaaggcga
     7981 gagagcaaag tgggacgcgg cgtgattatt ggtatttacg cgacggcccg gcgcgttagc
     8041 ggcccttccc ccaggccagg gacgattatg tatcaatatt gttgcgttcg ggcactcgtg
     8101 cgagggctcc tgcgggctgg ggagggggat ctgggaattg gaggtacgac cgagatggct
     8161 tgct
//
