LOCUS       40924_28416        6550 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Baculovirus expression vector pLM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Mei L.
  TITLE     Construction of high-efficiency baculovirus expression vector and 
            its expression in chicken cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Mei L.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2009) Microbiology, Life Science, Xuefu Road, 
            Harbin, Heilongjiang 150080, China
REFERENCE   3  (bases 1 to 6550)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6550)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-MAY-2009) Microbiology, Life Science, Xuefu Road, Harbin, 
            Heilongjiang 150080, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(8..129)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(236..824)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     misc_feature    complement(828..873)
                     /label=MCS
                     /note="MCS"
     promoter        complement(895..1098)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1099..1402)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1569..1660
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     1757..1816
                     /label=GP64 signal sequence
                     /note="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
     CDS             1991..2023
                     /codon_start=1
                     /label=VSV-G tag
                     /note="epitope tag from vesicular stomatitis virus G
                     protein"
                     /translation="YTDIEMNRLGK"
     polyA_signal    2154..2288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(2317..2482)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2666..3121
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3148..3252
                     /label=AmpR promoter
     CDS             3253..4110
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4284..4872
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(5175..5399)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5469..5999)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(6188..6216)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 cggtccgtaa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
       61 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
      121 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
      181 gaggtttttt aaagcaagta aaacctctac aaatgtggta tgctgattaa aacaggcggg
      241 gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc
      301 cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg
      361 tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccatggaaag
      421 gacgtcagct tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc
      481 cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg
      541 aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca
      601 gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga
      661 gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca
      721 tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga
      781 ataccagtca atctttcaca aattttgtaa tccagaggtt gattaacgtc gacaagcatg
      841 cgtgaattcc ggagctctgc tcgagcgagg cctgatctga cggttcacta aaccagctct
      901 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
      961 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     1021 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     1081 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     1141 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     1201 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     1261 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     1321 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     1381 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
     1441 aattgattac tattaataac tatctagatt cgcatcctcg gttttctgga aggcgagcat
     1501 cgtttgttcg cccaggactc tagctatagt tctagtggtt ggctacgtat actccggaat
     1561 attaatagat catggagata attaaaatga taaccatctc gcaaataaat aagtatttta
     1621 ctgttttcgt aacagttttg taataaaaaa acctataaat attccggatt attcataccg
     1681 tcccaccatc gggcgcggat ccatgctact agtaaatcag tcacaccaag gcttcaataa
     1741 ggaacacaca agcaagatgg taagcgctat tgttttatat gtgcttttgg cggcggcggc
     1801 gcattctgcc tttgcggata ctgggctatc caaaaatcca atcgagcttg tagaaggttg
     1861 gttcagtagt tggaaaagct ctattgcctc ttttttcttt atcatagggt taatcattgg
     1921 actattcttg gttctccgag ttggtatcca tctttgcatt aaattaaagc acaccaagaa
     1981 aagacagatt tatacagaca tagagatgaa ccgacttgga aagtaagctt gtcgagaagt
     2041 actagaggat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     2101 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     2161 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     2221 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     2281 tctggatctg atcactgctt gagcctagga gatccgaacc agataagtga aatctagttc
     2341 caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat
     2401 ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc
     2461 ccaactattt tgtccgccca cagcggggca tttttcttcc tgttatgttt ttaatcaaac
     2521 atcctgccaa ctccatgtga caaaccgtca tcttcggcta ctttttctct gtcacagaat
     2581 gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat atcaacgctt
     2641 atttgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2701 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2761 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2821 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2881 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     2941 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3001 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3061 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     3121 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3181 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3241 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3301 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3361 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     3421 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     3481 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3541 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3601 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3661 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3721 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3781 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3841 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     3901 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3961 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     4021 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     4081 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4141 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4201 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4261 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4321 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4381 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     4441 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4501 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4561 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4621 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     4681 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4741 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4801 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4861 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4921 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4981 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     5041 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     5101 caccgcagac cagccgcgta acctggcaaa atcggttacg gttgagtaat aaatggatgc
     5161 cctgcgtaag cgggtgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac
     5221 tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg
     5281 ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt
     5341 gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg catggtaaag actatattcg
     5401 cggcgttgtg acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg
     5461 aattgttagg tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc
     5521 aactttgtat agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca
     5581 taagcaccaa gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc
     5641 tgcctccggt gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc
     5701 tcaaacctgg gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca
     5761 gcaagcgcga tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga
     5821 tgttgggagt aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc
     5881 atggatttga cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca
     5941 cctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct gcgtaacatc
     6001 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     6061 ccgaggcata gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
     6121 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt
     6181 gcattacagt ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct tcatccgttt
     6241 ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg
     6301 gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt caggcattgg cggccttgct
     6361 gttcttctac ggcaaggtgc tgtgcacgga tctgccctgg cttcaggaga tcggaagacc
     6421 tcggccgtcg cggcgcttgc cggtggtgct gaccccggat gaagtggttc gcatcctcgg
     6481 ttttctggaa ggcgagcatc gtttgttcgc ccaggactct agctatagtt ctagtggttg
     6541 gctacgatcc
//
