LOCUS       40924_28411        6941 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Baculovirus expression vector pLM-ITRs, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Mei L.
  TITLE     Construction of high-efficiency baculovirus expression vector and 
            its expression in chicken cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Mei L.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2009) Microbiology, Life Science, Xuefu Road, 
            Harbin, Heilongjiang 150080, China
REFERENCE   3  (bases 1 to 6941)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6941)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-MAY-2009) Microbiology, Life Science, Xuefu Road, Harbin, 
            Heilongjiang 150080, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   32..172
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     polyA_signal    complement(189..310)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(417..1005)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     misc_feature    complement(1009..1054)
                     /label=MCS
                     /note="MCS"
     promoter        complement(1076..1279)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1280..1583)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     repeat_region   1690..1830
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     promoter        1960..2051
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     sig_peptide     2148..2207
                     /label=GP64 signal sequence
                     /note="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
     CDS             2382..2414
                     /codon_start=1
                     /label=VSV-G tag
                     /note="epitope tag from vesicular stomatitis virus G
                     protein"
                     /translation="YTDIEMNRLGK"
     polyA_signal    2545..2679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(2708..2873)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      3057..3512
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3539..3643
                     /label=AmpR promoter
     CDS             3644..4501
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4675..5263
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(5566..5790)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5860..6390)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(6579..6607)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 cggtccggga gaaaataccg catcaggcgc ccctgcaggc agctgcgcgc tcgctcgctc
       61 actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc ggcctcagtg
      121 agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc ctgcggccgc
      181 tcggtccgta agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
      241 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
      301 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
      361 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atgctgatta aaacaggcgg
      421 ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac gcggaagagg
      481 ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg ccgaagggac
      541 gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca gccatggaaa
      601 ggacgtcagc ttccccgaca acaccacgga attgtcagtg cccaacagcc gagcccctgt
      661 ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga gggggaaagc
      721 gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc
      781 agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact cctcataaag
      841 agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg aagcaatagc
      901 atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa catagttaag
      961 aataccagtc aatctttcac aaattttgta atccagaggt tgattaacgt cgacaagcat
     1021 gcgtgaattc cggagctctg ctcgagcgag gcctgatctg acggttcact aaaccagctc
     1081 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
     1141 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
     1201 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
     1261 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
     1321 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     1381 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     1441 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     1501 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     1561 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     1621 taattgatta ctattaataa ctatctagag taattgatta ctattaataa ctagtacgcg
     1681 tgcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     1741 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     1801 tgagcgagcg agcgcgcagc tgcctgcagg acatgtgagc aaaaggccag caatctagat
     1861 tcgcatcctc ggttttctgg aaggcgagca tcgtttgttc gcccaggact ctagctatag
     1921 ttctagtggt tggctacgta tactccggaa tattaataga tcatggagat aattaaaatg
     1981 ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa
     2041 aacctataaa tattccggat tattcatacc gtcccaccat cgggcgcgga tccatgctac
     2101 tagtaaatca gtcacaccaa ggcttcaata aggaacacac aagcaagatg gtaagcgcta
     2161 ttgttttata tgtgcttttg gcggcggcgg cgcattctgc ctttgcggat actgggctat
     2221 ccaaaaatcc aatcgagctt gtagaaggtt ggttcagtag ttggaaaagc tctattgcct
     2281 cttttttctt tatcataggg ttaatcattg gactattctt ggttctccga gttggtatcc
     2341 atctttgcat taaattaaag cacaccaaga aaagacagat ttatacagac atagagatga
     2401 accgacttgg aaagtaagct tgtcgagaag tactagagga tcataatcag ccataccaca
     2461 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     2521 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     2581 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     2641 ttgtccaaac tcatcaatgt atcttatcat gtctggatct gatcactgct tgagcctagg
     2701 agatccgaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt
     2761 attagcttac gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc
     2821 ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc acagcggggc
     2881 atttttcttc ctgttatgtt tttaatcaaa catcctgcca actccatgtg acaaaccgtc
     2941 atcttcggct actttttctc tgtcacagaa tgaaaatttt tctgtcatct cttcgttatt
     3001 aatgtttgta attgactgaa tatcaacgct tatttgcagc ctgaatggcg aatgggacgc
     3061 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     3121 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     3181 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     3241 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     3301 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     3361 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     3421 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     3481 gaattttaac aaaatattaa cgtttacaat ttcaggtggc acttttcggg gaaatgtgcg
     3541 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     3601 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     3661 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     3721 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     3781 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     3841 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     3901 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     3961 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4021 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4081 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4141 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4201 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4261 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4321 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4381 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4441 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     4501 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     4561 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     4621 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     4681 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     4741 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4801 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     4861 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4921 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4981 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5041 cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg aagggagaaa
     5101 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5161 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5221 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5281 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5341 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5401 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     5461 ttttctcctt acgcatctgt gcggtatttc acaccgcaga ccagccgcgt aacctggcaa
     5521 aatcggttac ggttgagtaa taaatggatg ccctgcgtaa gcgggtgtgg gcggacaata
     5581 aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa actagacaga
     5641 atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg acttttgtta
     5701 tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc
     5761 gtggccaagg gcatggtaaa gactatattc gcggcgttgt gacaatttac cgaacaactc
     5821 cgcggccggg aagccgatct cggcttgaac gaattgttag gtggcggtac ttgggtcgat
     5881 atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca ctgcgggatc
     5941 gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg cctcatgctt
     6001 gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg tgctcgccgg agactgcgag
     6061 atcatagata tagatctcac tacgcggctg ctcaaacctg ggcagaacgt aagccgcgag
     6121 agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct tactacggag
     6181 caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta cgtctccgaa
     6241 ctcacgaccg aaaagatcaa gagcagcccg catggatttg acttggtcag ggccgagcct
     6301 acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc gactgccctg
     6361 ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     6421 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt
     6481 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     6541 tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt
     6601 atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg
     6661 gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct
     6721 ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg
     6781 atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc
     6841 tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg
     6901 cccaggactc tagctatagt tctagtggtt ggctacgatc c
//
