LOCUS       V014534                 8522 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014534
VERSION     V014534
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    174..195
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        210..240
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    248..264
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     272..288
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        309..327
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        355..581
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    809..934
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1427..1660
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1845..1889
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2038..2079
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2156..2273
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2350..2653
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2654..2857
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2895..3602
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             3669..4697
                     /label="Cre"
                     /note="site-specific recombinase"
     misc_feature    4707..5295
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5448..5628
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5700..5834
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5861..5996
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6017..6035)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6045..6061)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6203..6658
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6684..6788
                     /label="AmpR promoter"
     CDS             6789..7646
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7820..8408
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
       61 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      121 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
      181 agttagctca ctcattaggc accccaggtt ttacacttta tgcttccggc tcgtatgttg
      241 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
      301 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
      361 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
      421 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
      481 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
      541 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
      601 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
      661 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
      721 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
      781 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
      841 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
      901 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
      961 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     1021 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     1081 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     1141 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     1201 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     1261 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     1321 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1381 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1441 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1501 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1561 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1621 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1681 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1741 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1801 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1861 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1921 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1981 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     2041 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     2101 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta
     2161 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     2221 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgatc
     2281 acgagactag cctcgataat agtaatcaat tacggggtca ttagttcata gcccatatat
     2341 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2401 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2461 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2521 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2581 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2641 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2701 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2761 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     2821 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg
     2881 ctagcgctac cggtatggtg agcaagggcg aggaggataa catggccatc atcaaggagt
     2941 tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg
     3001 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     3061 gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg
     3121 cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct
     3181 tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact
     3241 cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct
     3301 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt
     3361 accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg
     3421 gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg
     3481 gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg
     3541 tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca
     3601 agggatctgg agcaacaaac ttctcactac tcaaacaagc aggtgacgtg gaggagaatc
     3661 ccggccctat gtccaattta ctgaccgtac accaaaattt gcctgcatta ccggtcgatg
     3721 caacgagtga tgaggttcgc aagaacctga tggacatgtt cagggatcgc caggcgtttt
     3781 ctgagcatac ctggaaaatg cttctgtccg tttgccggtc gtgggcggca tggtgcaagt
     3841 tgaataaccg gaaatggttt cccgcagaac ctgaagatgt tcgcgattat cttctatatc
     3901 ttcaggcgcg cggtctggca gtaaaaacta tccagcaaca tttgggccag ctaaacatgc
     3961 ttcatcgtcg gtccgggctg ccacgaccaa gtgacagcaa tgctgtttca ctggttatgc
     4021 ggcggatccg aaaagaaaac gttgatgccg gtgaacgtgc aaaacaggct ctagcgttcg
     4081 aacgcactga tttcgaccag gttcgttcac tcatggaaaa tagcgatcgc tgccaggata
     4141 tacgtaatct ggcatttctg gggattgctt ataacaccct gttacgtata gccgaaattg
     4201 ccaggatcag ggttaaagat atctcacgta ctgacggtgg gagaatgtta atccatattg
     4261 gcagaacgaa aacgctggtt agcaccgcag gtgtagagaa ggcacttagc ctgggggtaa
     4321 ctaaactggt cgagcgatgg atttccgtct ctggtgtagc tgatgatccg aataactacc
     4381 tgttttgccg ggtcagaaaa aatggtgttg ccgcgccatc tgccaccagc cagctatcaa
     4441 ctcgcgccct ggaagggatt tttgaagcaa ctcatcgatt gatttacggc gctaaggatg
     4501 actctggtca gagatacctg gcctggtctg gacacagtgc ccgtgtcgga gccgcgcgag
     4561 atatggcccg cgctggagtt tcaataccgg agatcatgca agctggtggc tggaccaatg
     4621 taaatattgt catgaactat atccgtaacc tggatagtga aacaggggca atggtgcgcc
     4681 tgctggaaga tggcgattag gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     4741 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4801 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4861 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4921 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4981 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     5041 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     5101 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5161 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5221 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5281 gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc
     5341 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5401 ccaacgaaga caagatatcc tgctagctcc ggatccggac ctgtactggg tctctctggt
     5461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     5521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     5581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     5641 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     5701 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5761 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5821 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     5881 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5941 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6001 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     6061 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     6121 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     6181 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6241 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6301 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6361 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6421 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6481 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6541 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6601 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     6661 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     6721 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6781 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6841 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6901 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6961 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     7021 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     7081 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     7141 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     7201 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     7261 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     7321 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     7381 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     7441 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7501 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7561 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7621 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7681 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7741 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7801 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7861 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7921 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7981 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     8041 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     8101 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     8161 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     8221 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     8281 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8341 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8401 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8461 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8521 ag
//
