LOCUS       V000690                 9431 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V000690
VERSION     V000690
KEYWORDS    pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Bajar BT, Lam AJ, Badiee RK, Oh YH, Chu J, Zhou XX, Kim N, Kim BB,
            Chung M, Yablonovitch AL, Cruz BF, Kulalert K, Tao JJ, Meyer T, Su
            XD, Lin MZ
  TITLE     Fluorescent indicators for simultaneous reporting of all four cell
            cycle phases.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4045.
   PUBMED   27798610
REFERENCE   2  (bases 1 to 9431)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9431)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Methods. 2016 Oct 31. doi: 10.1038/nmeth.4045."
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    13..563
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             577..1158
                     /gene="H1-0"
                     /label="Histone H1.0"
                     /note="Histone H1.0 from Mus musculus. Accession#: P10922"
     CDS             1189..1899
                     /label="mMaroon1"
                     /note="bright, rapidly maturing, monomeric far-red
                     fluorescent protein (Bajar et al., 2016)"
     protein_bind    1930..1963
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     misc_feature    2019..2607
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(2610..2626)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     LTR             2849..3029
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(3091..3679)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3853..4710)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4711..4815)
                     /label="AmpR promoter"
     enhancer        4906..5209
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5211..5409
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             5427..5607
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5654..5779
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    6276..6509
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             6694..6738
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             6887..6928
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    7036..7153
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        7212..7524
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das
                     et al., 1988)"
     primer_bind     7541..7557
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(7599..7632)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        7748..8051
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8052..8255
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             8291..9007
                     /label="mTurquoise2"
                     /note="enhanced monomeric variant of CFP (Goedhart et al.,
                     2012)"
     misc_feature    9421..9431
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
       61 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
      121 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
      181 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
      241 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
      301 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
      361 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
      421 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
      481 catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt
      541 cctttgaaaa acacgatgat aatggccaca gaattcatga ccgagaactc cacctccgcc
      601 ccggcggcga agcccaaacg ggccaaggct tccaagaagt ccacggacca ccccaagtat
      661 tcagacatga tcgtggctgc tatccaggca gagaagaacc gtgccggctc ctcgcgccag
      721 tccatccaaa agtatatcaa gagccactac aaggtgggtg agaacgccga ctcccagatc
      781 aagttgtcca tcaagcgcct agtgaccacc ggtgttctca agcaaaccaa aggggtgggc
      841 gcctcggggt ccttcaggct ggccaagggc gatgagccca aaaggtcggt ggctttcaag
      901 aagaccaaga aggaagtcaa gaaagtggcc actccaaaga aggcagccaa gcccaagaag
      961 gctgcctcca aagccccaag caagaaaccc aaagccaccc ctgtcaagaa ggccaagaag
     1021 aagccggctg ccacgcccaa gaaagccaaa aagcccaagg ttgtcaaagt caaaccagtc
     1081 aaggcctcca aacccaagaa ggccaaaacc gtgaagccca aagccaagtc gagtgccaag
     1141 agggggagca agaagaagtc cggactcaga tctggaggct ccggaggcat ggtgagcaag
     1201 ggcgaggagc tgatcaagga gaacatgcac accaagctgt acctgacagg caccgtgaac
     1261 aaccactact tcgaatgcac cgccgaaggg gagggcaagc cctacgaggg cacccagacc
     1321 aacaggatca aggtggtgcg cggaggcccc ctgccgttcg cattcgacat cctggccccc
     1381 tgctttatgt acgggagcaa gaccttcatt aaccatccac ctgacattcc cgattatttt
     1441 aagcagtcct tccctgaggg cttcacatgg gagagaacca ccgtttacga agacgggggc
     1501 acactcactg ctacacagga caccagcctc caggacggct gcctgatcta caacgtccaa
     1561 gtcagagggg agaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg
     1621 gaggccagta ccgagaccct gtaccccgct gacggcagcc tggaaggcag actggactgg
     1681 gccctgaagc tcgtgggcgg gggccacctg cactgcagac tggaaaccac atacagatcc
     1741 aagaaacccg caaagaacct caagatgccc ggcgtctatt ttattgacag gagactggaa
     1801 agaatcaagg aggccgacaa tgagacctac gtcgagcagc acgaggtggc tgtggccaga
     1861 tactgcgacc tccctagcaa actggggcac aaacttaatt gagtttaaac gaattcgtcg
     1921 agggacctaa taacttcgta tagcatacat tatacgaagt tatacatgtt taagggttcc
     1981 ggttccacta ggtacaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     2041 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     2101 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     2161 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     2221 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     2281 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     2341 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     2401 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     2461 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     2521 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     2581 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgatcg agacctagaa
     2641 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     2701 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     2761 atgacttaca aggcagctga cgcgtgattt taaaagaaaa ggggggactg gaagggctaa
     2821 ttcactccca ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     2881 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     2941 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     3001 cttttagtca gtgtggaaaa tctctagcag catgtgagca aaaggccagc aaaaggccag
     3061 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3121 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3181 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3241 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3301 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3361 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3421 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3481 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3541 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3601 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3661 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3721 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     3781 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     3841 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     3901 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     3961 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4021 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4081 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4141 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4201 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4261 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4321 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4381 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4441 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4501 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4561 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4621 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4681 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4741 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4801 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacactagtt attaatagta
     4861 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     4921 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     4981 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     5041 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     5101 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     5161 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     5221 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     5281 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     5341 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     5401 tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     5461 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     5521 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     5581 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     5641 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     5701 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     5761 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     5821 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     5881 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     5941 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     6001 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     6061 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     6121 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     6181 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     6241 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     6301 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     6361 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     6421 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     6481 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     6541 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     6601 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     6661 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     6721 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     6781 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     6841 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     6901 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     6961 agatccattc gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc
     7021 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     7081 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     7141 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttggt tagtaccggg
     7201 cccgctctag agatccgacg cgccatctct aggcccgcgc cggccccctc gcacagactt
     7261 gtgggagaag ctcggctact cccctgcccc ggttaatttg catataatat ttcctagtaa
     7321 ctatagaggc ttaatgtgcg ataaaagaca gataatctgt tctttttaat actagctaca
     7381 ttttacatga taggcttgga tttctataag agatacaaat actaaattat tattttaaaa
     7441 aacagcacaa aaggaaactc accctaactg taaagtaatt gtgtgttttg agactataaa
     7501 tatcccttgg agaaaagcct tgttaacgcg cggtgaccct cgaggtcgac ggtatcgata
     7561 agctcgcttc acgagattcc agcaggtcga gggacctaat aacttcgtat agcatacatt
     7621 atacgaagtt atattaaggg ttccaagctt aagcggccgc gtggataacc gtattaccgc
     7681 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     7741 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     7801 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     7861 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     7921 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     7981 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     8041 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     8101 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     8161 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     8221 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
     8281 agcaccaggt atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     8341 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     8401 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     8461 ctggcccacc ctcgtgacca ccctgagctg gggcgtgcag tgcttcgccc gctaccccga
     8521 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     8581 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     8641 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     8701 cctggggcac aagctggagt acaactactt cagcgacaac gtctatatca ccgccgacaa
     8761 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt
     8821 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     8881 cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga
     8941 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     9001 gtacaagtac tcaatgttgc cttccctggg aggataccca tcacactggc gaccactcga
     9061 ggccagcccg ccgtcccccg cgcgatggag cctgggacgg aagcgcagag ccgacggcag
     9121 gcgctggagg cccgaagacg ccgaggaggc agagcaccgc ggcgccgagc gcagacccga
     9181 gagctttacc actcctgaag gccctaaacc ccgttccaga tgctctgact gggcaagtgc
     9241 agttgaagaa gatgaaatga ggaccagagt taacaaagaa atggcaagat ataaaaggaa
     9301 actcctcatc aatgactttg gaagagagag aaaatcatca tcaggaagtt ctgattcaaa
     9361 ggagtctatg tctactgtgc cggctgacga tcgaggatga agaaggatct tagcttaagg
     9421 cccctctccc t
//
